2results about How to "Regulate immune response" patented technology

This invention discloses a method for preparing purslane exosomes with anti-allergic effects and their applications, specifically relating to the field of natural active substances. The method includes washing fresh purslane and then juicing or homogenizing it, separating solid residue from the liquid juice to obtain a plant homogenate supernatant as a precursor. The precursor is then subjected to low-temperature differential centrifugation to remove cell debris and large particulate impurities, and the clarified supernatant is collected. The clarified supernatant is then filtered through one or more multi-stage filter membranes, with pore sizes selected from 5 μm, 1.2 μm, 0.45 μm, 0.22 μm, and 100 nm. Through optimized extraction processes, such as specific enzymatic hydrolysis steps, centrifugation conditions, and ultrafiltration technology, the extraction efficiency and purity of exosome-like nanovesicles are significantly improved. Compared to traditional extraction methods, this method can more accurately remove impurities, preserve the integrity and bioactivity of exosome-like nanovesicles, and ensure their stability and effectiveness in subsequent applications.

This invention provides a high-throughput and efficient method for screening multiple target genes that can regulate mRNA expression levels in cells by co-transfecting or sequentially transfecting mRNA with a gene silencing library targeting genes in cells using lipid nanoparticles. Several target genes that can regulate mRNA protein expression levels through the action of siRNA have been discovered. By manipulating these genes that regulate mRNA expression levels, the translational capacity of mRNA in mRNA vaccine and drug development can be improved, mRNA protein expression efficiency can be increased, and immunogenicity and toxicity can be reduced.