The invention provides a phase type SPR detection device and method based on
white light interference spectrum. The device includes: a
light source, a filter polarization module, a wave plate, an SPR sensing module, an area array
detector and a control terminal. In the present invention, the wave plate is used to generate phase
delay for the P-polarized light and S-polarized light in the incident light, and a spectral
interference phenomenon occurs; in order to realize high-
throughput sensing, an optical-frequency time-division
multiplexing technology is adopted, and an area array
detector is used to sense Surface imaging, obtain the
white light interference spectrum of all sites, and analyze the
white light interference spectrum in the
frequency domain to obtain the
resonance wavelength corresponding to samples with different refractive indices, and then select the effective sinusoidal interference spectrum through the
resonance wavelength, and use the
algorithm to obtain samples with different refractive indices in real time The corresponding
phase change can obtain the molecular interaction information of the sample to be tested. By simultaneously monitoring the SPR
phase change in different wave bands, the detection
dynamic range can be expanded, and no external modulator is required to modulate the
light wave, and the device structure is simple.