39results about How to "Large detection dynamic range" patented technology

The invention discloses a method for detecting miRNA (ribonucleic acid). The method comprises the steps: (1) synthesizing a DNA (deoxyribonucleic acid) tetrahedron probe; (2) connecting three peak points of the DNA tetrahedron probe to the surface of a working electrode of an electrochemical device to obtain a working electrode with a capture probe; (3) adding a target miRNAs to be detected, a signal probe and an auxiliary chain into a reaction system to implement hybridization reaction to form a composite body I, and immersing the working electrode into the reaction system to implement hybridization reaction to form a composite body II; (4) generating reaction between the composite body II and enzyme capable of generating catalytic oxidation reduction reaction; (5) adding a primer produced by the enzyme catalytic oxidation to realize electrochemical detection analysis. The target miRNAs can be directly detected by the method disclosed by the invention without being marked and subjected to pre-PCR (polymerase chain reaction) multiplication; the method is easy to operate, so that the experiment cost is greatly reduced, and the experiment efficiency is improved.

The invention relates to a receiving system which can be applied to a laser radar. The system comprises a receiving lens which is configured to receive and converge light beams reflected by an obstacle; an optical modulator having an ON state and an OFF state, disposed at the downstream of an optical path of the receiving lens, and receiving the light beams converged by the receiving lens; and a detector arranged at the downstream of the optical modulator. In the ON state, the optical modulator is configured to allow the converged light beams to input on the detector, and the in the OFF state,the converged light beam is not allowed to input onto the detector.

The invention discloses a double-stranded spire tetrahedral DNA nano-structural probe (STTS) as well as a preparation method and an application of the STTS in electrochemical detection of the activity of a telomerase. The STTS comprises a single-stranded probe STTS-A, a single-stranded probe B, a single-stranded probe C, a single-stranded probe D and a single-stranded probe SC-DNA. The invention also discloses a method for electrochemical detection of the activity of the telomerase by virtue of the STTS and the application of the STTS in electrochemical detection of the activity of the telomerase. By adopting the method for electrochemical detection of the activity of the telomerase, the signal to noise ratio of detected signals is more than twice higher than that of the detected signals detected the ordinary TSP (Test Sphere Probe) detection, the sensitivity is high, the specificity is strong and the application range is wide.

The invention discloses a configurable received signal strength indicating circuit and belongs to the technical field of integrated circuits. The circuit comprises a subtracter, a limiting amplifier chain, a full-wave rectifier, an output buffer and a direct-current detuning extract circuit, wherein the direct-current detuning extract circuit is used for extracting a direct-current detuning voltage; the subtracter is used for subtracting an input intermediate-frequency signal and the direct-current detuning voltage and respectively outputting the obtained signals to the limiting amplifier chain and the full-wave rectifier; the output signal of each limiting amplifier in the limiting amplifier chain can be input into the full-wave rectifier; the output signals of the limiting amplifier chain can be respectively inputted in the direct-current detuning extract circuit and the output buffer; the output buffer outputs the intermediate-frequency signal, and the full-wave rectifier outputs a received signal strength indicating signal. The configurable received signal strength indicating circuit provided by the invention can occupy small layout area and work stably and reliably, can be applied to low-intermediate frequency and zero-intermediate frequency receivers at the same time, and is especially applicable to the zero-intermediate frequency application.

The invention discloses a receiving signal strength indicating circuit with layout area reduced. The circuit comprises a subtractor, a limiting amplifier chain, a full-wave rectifier set, an output buffer and a direct-current offset extraction circuit. By adopting the novel direct-current offset extraction circuit, the layout area of the whole circuit is substantially reduced, integration level is improved, and cost is lowered. The receiving signal strength indicating circuit with the layout area reduced is simple in structure, stable and reliable in working, capable of being applied to low-intermediate-frequency and zero-intermediate-frequency receivers, and especially suitable for zero-intermediate-frequency application.

The invention discloses an acousto-optic adjustable filtering type near-infrared spectrometer comprising a light source module, a light condensation and collimation system I, an acousto-optic adjustable filtering device, a light condensation and collimation system II, a light condensation system, a photoelectric detector and a signal collecting and processing system, wherein the signal collecting and processing system is sequentially connected with a regulating and controlling pulse generator and a radio frequency driving source; the output of the radio frequency driving source is connected with the acousto-optic adjustable filtering device; the signal collecting and processing system generates a numerical control signal to the regulating and controlling pulse generator; the regulating and controlling pulse generator generates a regulating and controlling signal with suitable duty ratio and transmits the regulating and controlling signal with suitable duty ratio to the radio frequency driving source so as to be subjected to radio frequency signal modulation and controlling; and the radio frequency driving source amplifies the modulated and controlled radio frequency signal to a suitable power so as to drive the acousto-optic adjustable filtering device. The acousto-optic adjustable filtering type near-infrared spectrometer has the following beneficial effects: 1, the spectral resolution is high; 2, the detection sensitivity is high; 3, the detection dynamic range is large and the adaptability to various samples is strong; and 4, the signal can be processed and collected in real time and the environmental adaptability is strong.

The invention discloses a cloth chip gravity/capillary flow chemiluminescence method and particularly discloses a gravity/capillary flow coupled fluid driving cloth chip and application thereof in heavy metal ion detection. The cloth chip is divided into a hydrophobic area and a hydrophilic area, the hydrophilic area is further divided into three parts of a sampling area, a detection area and a fluid channel area. When the cloth chip is used, the cloth chip is closely placed on a support, the inclined plane of the support and a horizontal plane form an included angle. After the cloth chip is arranged, the sampling area and the fluid channel area are required to be located on the inclined plane of the support, the detection area is located at the horizontal portion of the support, and meanwhile a hydrophobic sheet is cushioned under in the detection area of the cloth chip. Compared with traditional flow chemiluminescence detection, the cloth chip and a detection method do not need any expensive pump device (such as a precision injection pump and a peristaltic pump) for driving liquid to flow. The cloth chip gravity/capillary flow chemiluminescence method only utilizes the natural gravity of liquid and capillary force of cloth fiber gaps to drive the liquid to flow.

This invention relates to a near field enhanced Raman molecular fingerprint spectrum analysis method<0}, which comprises an optimized near-field reinforced Raman sample pond, an inverted or vertical microscope, and a Raman spectrometer. The analysis can comprise five steps or less according to request, which comprises mainly: preparing the sample into liquid for direct use, or taking full 2D gel electrophoretic separation; comparing the determined data with database. This invention special fit to active protein constituent analysis and medical research.

The invention discloses a closed type bipolar electrode electrogenerated chemiluminescence fabric chip, and a preparation method and application thereof. The substrate of the chip is a fabric piece on which electrodes are distributed, a bipolar electrode is at the center, and a pair of drive electrodes are distributed at two sides; the positive drive electrode is communicated with the cathode of the bipolar electrode via a support microchannel, and the negative driving electrode is communicated with the anode of the bipolar electrode via a report microchannel; and a hydrophobic wax dam is arranged round the microchannels, and the microchannels are hydrophilic. The chip possesses the potential for preparing an ultra-flexible sensor because of extremely good flexibility. Additionally, compared with a conventional chip substrate material, the fabric chip possesses the advantages of cheapness, universality, friendliness to users, good biocompatibility, biodegradability and the like.

The invention provides a phase type SPR detection device and method based on a white light interference spectrum. The device comprises a light source, a filtering polarization module, a wave plate, anSPR sensing module, an area array detector and a control terminal. Phase delay is generated on P polarized light and S polarized light in incident light through the wave plate, and the spectral interference phenomenon occurs; to achieve high throughput sensing, an optical frequency time division multiplexing technology is adopted; the area array detector is used for imaging the sensing surface; white light interference spectrums of all sites are obtained; frequency domain analysis is carried out on the white light interference spectrum; resonance wavelengths corresponding to samples with different refractive indexes are obtained; an effective sine interference spectrum is selected through resonance wavelength, phase changes corresponding to samples with different refractive indexes are obtained in real time through an algorithm, interaction information of molecules of a sample to be detected is obtained, the SPR phase changes are monitored at the same time in different wave bands, thedetection dynamic range is expanded, an external modulator is not needed for modulating light waves, and the device is simple in structure.

The invention discloses a tunable long-range SPR device and a method for detecting the refractive index using the same. The device includes a laser, a lens, a prism, an LRSPR sensor chip, a detectionmedium layer, a unit photodetector, an adjustable voltage output device and a data processing system, wherein the LRSPR sensor chip sequentially includes an oxide conductive layer prepared on a bottomsurface of the prism, an external field modulation medium layer, a buffer medium layer, a metal functional layer, a surface modification layer and a sample cell, the adjustable voltage output deviceis controlled by the data processing system, the sample cell is used for accommodating the detection medium layer, the sample cell is set on at a lower surface of the surface modification layer, a gapis formed between the sample cell and the surface modification layer, and the liquid level of the detection medium layer is in contact with the lower surface of the surface modification layer. The device is advantaged in that device has a simple structure, can calculate the change of the refractive index of the detection medium layer by measuring signals of an external field, has the fast detection response speed and the large detection dynamic range, and improves practicality of the sensor.

The invention discloses a water flow speed measurement radar which is characterized by comprising an antenna, a time delay reconstruction radio frequency TR radio frequency module and a radio frequency sampling signal processing module. The time delay reconstruction radio frequency TR radio frequency module comprises a radio frequency signal receiving channel, a radio frequency signal transmitting channel and a change-over switch. One of the radio frequency signal receiving channel and the radio frequency signal transmitting channel is selected to be connected with the antenna through the change-over switch; the radio frequency signal receiving channel comprises a first low-noise amplifier and a programmable delay line, and amplification of received signals and program control configuration of fixed deviation time delay are achieved through the first low-noise amplifier and the programmable delay line. The radar has the characteristics of high water flow velocity measurement resolution, high sampling point density, large detection dynamic range, full intelligence, wide applicability and the like.

The invention provides a cell strain for detecting the activity of an antibody-immune agonist coupled drug and a detection method. The cell strain expresses an antigen or a fragment thereof capable of being combined with an antibody in the antibody-immune agonist coupled drug, a receptor capable of being combined with an immune agonist in the antibody-immune agonist coupled drug, and an immune signal path reporter gene. The cell strain provided by the invention can be used for activity detection of immune agonist coupled drugs with different target antigens and different activity levels. The detection method disclosed by the invention can overcome the defects of a PBMC and antigen positive cell co-culture mode, and has the advantages of simplicity in operation, low cost, higher sensitivity, larger detection dynamic range and the like; and the activity result of the antibody-immune agonist coupled drug obtained by the detection method is closer to the verification result of an in-vivo pharmacological experiment.

The invention discloses a partial discharge positioning method based on electroacoustic joint detection signal propagation time delay compensation. The method comprises the steps of: carrying out sampling and extraction on partial discharge ultrahigh frequency electromagnetic waves and ultrasonic acoustic signals, and obtaining electromagnetic wave pulses and sound pulse signals generated by a partial discharge source; respectively extracting propagation time delay characteristics of the two kinds of signals; taking the propagation time delay characteristic value of the electromagnetic wave signals as a reference datum, obtaining the relative propagation time delay of the ultrasonic wave signals, and carrying out correction compensation on the relative propagation time delay under a metal boundary condition; calculating the distance between the partial discharge source and an ultrasonic sensor; repeatedly sampling for multiple times to calculate the distance between the partial discharge source and the single ultrasonic sensor, and taking the arithmetic mean value of the distance as the finally judged distance between the partial discharge source and the ultrasonic sensor; and finally, determining the specific position of the partial discharge source on the basis of electrical arrangement in a cabinet. According to the positioning method, the accurate position of the partial discharge source of equipment can be embodied more accurately.