A phase-type spr detection device and method based on interference spectroscopy

Abstract

The invention provides a phase-type SPR detection device and method based on interference spectrum. The device includes: a light source, a polarizer, a wave plate, an SPR sensing module, a spectrometer and a control terminal. The invention utilizes a wave plate with a certain thickness to delay the phase of the P-polarized light and the S-polarized light in the incident light, so that the phenomenon of spectral interference occurs; the SPR resonance wavelength and phase are analyzed in real time by performing a frequency domain-time domain joint algorithm on the interference spectrum. , the SPR phase extraction accuracy is improved; by obtaining the resonance wavelengths of samples with different refractive indices, the effective interference spectrum is obtained through the resonance wavelength, and then the SPR phase change is extracted from the effective interference spectrum, and the sample to be tested is obtained according to the SPR phase change. Compared with the traditional phase modulation SPR technology, SPR phase detection with a large dynamic range is realized without any modulator, which has the advantages of strong anti-noise capability and low R&D cost.

Description

technical field [0001] The invention belongs to the technical field of optical sensing imaging, and in particular relates to a phase-type SPR detection device and method based on interference spectrum. Background technique [0002] Surface plasmon resonance (SPR) sensing technology has the advantages of label-free, real-time monitoring, low sample consumption, high sensitivity and high-throughput detection. It is used in environmental monitoring, food safety, genomics and proteomics research. and other fields have a wide range of applications. SPR is an optical phenomenon. Under certain conditions, light incident on some metal surfaces will be totally reflected. The evanescent wave incident on the metal will resonate with the surface plasmon wave on the metal surface. Optical parameters are related, and different types of SPR sensors are generated by identifying different resonant optical parameters, such as intensity modulation, angular modulation, spectral modulation and ...

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Problems solved by technology

[0005] In view of the deficiencies in the above-mentioned prior art, the object of the present invention is to provide a phase-type SPR detection device and method based on interference spectrum, which overcomes the small dynamic detection range of the existing phase-modulated SPR device and needs to introduce a modulator to detect the incident light. Or the reflected light is modulated or demodulated, which leads to the defects of complicated device structure and high cost

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