Darkroom for detecting colloidal-gold strip and using method thereof

Abstract

The invention provides a darkroom for detecting a colloidal-gold strip and a using method thereof. The darkroom for detecting the colloidal-gold strip comprises a sealed light-shading box formed by light-shading boards, a light-emitting diode and a photodiode; one side wall of the light-shading box is provided with a side wall opening for the strip to insert; a plurality of light-shading baffles which are parallel with the side wall of the light-shading box with the side wall opening and have the same size are arranged in the light-shading box; the light-shading baffles divide the light-shading box into mutually independent compartments and are provided with light-shading baffle openings, and the positions of the light-shading baffle openings correspond to the position of the side wall opening so as to facilitate horizontally inserting the strip into the light-shading box; and the light-emitting diode and the photodiode are arranged on the bottom of the light-shading box. The absorption spectrum range of the colloidal-gold strip is determined according to the particle size distribution of colloidal gold particles on the colloidal-gold strip to be detected, so that the light-emitting diode and the photodiode working in the spectrum range are selected. The detecting darkroom has the advantages of accurate detection of the concentration of an antigen spotted on the colloidal-gold strip, high detection sensitivity, objective and accurate result and flexible use.

Description

technical field [0001] The invention relates to a detection darkroom for a gold standard test strip and an application method thereof. Background technique [0002] At present, the research on test strips for rapid disease detection is progressing rapidly, and its detection methods are convenient, fast and accurate. Especially with the changes in Chinese dietary structure, lifestyle changes and social pressure, obesity, diabetes and high blood pressure are increasing, and the incidence of cancer is also increasing year by year. At the same time, many tumor markers related to cancer have been discovered one after another. Therefore, this test strip for qualitative detection has also been developed one after another, but it has several obvious shortcomings: [0003] 1. Only qualitative detection, not quantitative detection. The color of the test line on the test strip reflects the level of antigen concentration. However, visual observers generally can only judge the presenc...

AI Technical Summary

Problems solved by technology

However, visual observers can only judge the presence or absence of tumor markers based on the color, but cannot judge its concentration.

[0004] 2. The test results lack objectivity

Since the visual judgment of color is subjective, the judgment is greatly affected by experience and environment, and the judgment of the same test strip lacks repeatability.

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