Phase type SPR detection device and method based on white light interference spectrum

Abstract

The invention provides a phase type SPR detection device and method based on a white light interference spectrum. The device comprises a light source, a filtering polarization module, a wave plate, anSPR sensing module, an area array detector and a control terminal. Phase delay is generated on P polarized light and S polarized light in incident light through the wave plate, and the spectral interference phenomenon occurs; to achieve high throughput sensing, an optical frequency time division multiplexing technology is adopted; the area array detector is used for imaging the sensing surface; white light interference spectrums of all sites are obtained; frequency domain analysis is carried out on the white light interference spectrum; resonance wavelengths corresponding to samples with different refractive indexes are obtained; an effective sine interference spectrum is selected through resonance wavelength, phase changes corresponding to samples with different refractive indexes are obtained in real time through an algorithm, interaction information of molecules of a sample to be detected is obtained, the SPR phase changes are monitored at the same time in different wave bands, thedetection dynamic range is expanded, an external modulator is not needed for modulating light waves, and the device is simple in structure.

Description

technical field [0001] The invention belongs to the technical field of optical sensing and imaging, and in particular relates to a phase type SPR detection device and method based on white light interference spectrum. Background technique [0002] Surface plasmon resonance (Surface plasmon resonance, SPR) sensing technology has the advantages of label-free, real-time monitoring, low sample consumption, high sensitivity and high-throughput detection, and is widely used in environmental monitoring, food safety, genomics and proteomics research. and other fields have a wide range of applications. SPR is an optical phenomenon. Under certain conditions, light is incident on some metal surfaces and undergoes total reflection. The evanescent wave of the incident light incident on the metal resonates with the surface plasmon wave on the metal surface. The resonance conditions and the metal film surface Different types of SPR sensors are generated by identifying different resonant o...

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Problems solved by technology

[0005] In view of the deficiencies in the above-mentioned prior art, the object of the present invention is to provide a phase-type SPR detection device and method based on white light interference spectrum, which overcomes the need for an external modulator to modulate the light wave in the existing phase-modulated SPR detection device. The structure is complex, the cost of high-throughput detection is high, and SPR detects defects with a small dynamic range

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