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A cloth chip gravity/capillary flow chemiluminescence method

A gravity and chip technology, applied in the field of microfluidic analysis, can solve the problems of large volume, complex process, increased difficulty in chip design and processing, etc., and achieve the effect of good biocompatibility, simple processing method and easy use.

Active Publication Date: 2017-11-24
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, these reported flow chemiluminescence methods for microfluidic chips still have some disadvantages: (1) Almost all chemiluminescent chips are based on substrate materials such as glass, silicon, and polymers, which are used in microfabrication The process is relatively complicated; (2) The longer microchannels and delicately designed micromixers required for the reaction increase the difficulty of chip design and processing; (3) Similar to large-scale systems, PMTs as optical detectors not only require high voltage, And the volume is large; (4) Most flow chemiluminescence microfluidic chip systems require external pumps for liquid manipulation
For this reason, so far, no report on the detection of heavy metals or heavy metal ions by chemiluminescence has been found on the cloth chip.

Method used

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  • A cloth chip gravity/capillary flow chemiluminescence method
  • A cloth chip gravity/capillary flow chemiluminescence method
  • A cloth chip gravity/capillary flow chemiluminescence method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] A preparation method of a gravity / capillary force coupled fluid-driven cloth chip, comprising the steps of:

[0071] (a) Use Adobe Illustrator CS5 software to design such as figure 2 The pattern of the hydrophilic area of ​​the cloth chip (the white area is the hydrophilic area) is shown, and then based on these patterns, it is processed into a 300-mesh mesh screen by Guangzhou Lianchang Printing Equipment Store.

[0072] (b) Take a piece of white cotton cloth with a size of 50mm × 60mm, stick the screen on the cloth, paint on the screen with a purple crayon, and grind it evenly with a smooth grinding spoon; after grinding, put Put the cloth and the screen together on a heating plate (model YH-946B) at a temperature of 90°C and heat for about 5 seconds (the side with the cotton cloth faces the heating plate), the wax penetrates into the cloth to form a hydrophobic area, and the other areas are hydrophilic. water area. Then, the cloth sheet and the mesh plate are remo...

Embodiment 2

[0077] The application of the cloth chip of embodiment 1 in detecting Cr (III), comprises the following steps:

[0078] (a) Put the PET plastic bracket assembled with the cloth chip into the dark box, align the detection area of ​​the cloth chip with the macro lens (model M1614-MP) of the CCD camera (model MC15), adjust the CCD related parameters (such as exposure time, blue Chromatic light gain, etc.) and focal length to make the image clearest.

[0079] (b) Add 5-30 μL of Cr(III) test solution to the center of the detection area of ​​the cloth chip, and then add 5-40 μL of chemiluminescence base solution to the sample application area to trigger chemiluminescence.

[0080] (c) Using CCD camera video imaging GCF-CL process, the video imaging parameters are 15 frames per second, the bit rate is 3000, and through VGIF ( http: / / video-to-gif.watermark-software.com / ) software processes the imaging video during the period from the closure of the dark box to the end of chemilumin...

Embodiment 2

[0088] Several important factors (pH value, component concentration of the test solution and the bottom solution) that affect the luminous intensity of the cloth chip GCF-CL in Example 2 are optimized

[0089] a) Preferred EDTA concentration

[0090] 1. 30 μL test solution composition: Cr(III) concentration is 50 μg / mL, KBr concentration is 0.75M, EDTA concentration is to be determined, pH value is 3.0; 35 μL bottom solution composition: Luminol concentration is 3mM, H 2 o 2 The concentration is 0.1M, the pH value is 11.5.

[0091] 2. Set up several experimental groups: the concentration of EDTA in the GCF-CL test solution is set to several different values ​​(0mM, 1mM, 5mM, 10mM, 15mM, 20mM, 25mM).

[0092] 3. The detection process of the cloth chip GCF-CL is the same as in Example 2, and the test results are as follows Figure 9 shown.

[0093] It can be seen from the experimental results that when the concentration of EDTA is less than or equal to 10mM, the relative lig...

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Abstract

The invention discloses a cloth chip gravity / capillary flow chemiluminescence method, in particular discloses a cloth chip with gravity / capillary force coupling driving fluid and its application in detecting heavy metal ions. The cloth chip of the present invention is divided into a hydrophobic area and a hydrophilic area, and the hydrophilic area is further divided into three parts: a sampling area, a detection area and a fluid channel area; when in use, the cloth chip should be placed on a support closely, The inclined surface of the bracket forms an included angle with the horizontal plane. After placement, the sample loading area and fluid channel area should be on the inclined surface of the bracket, and the detection area should be on the horizontal part of the bracket. At the same time, a hydrophobic sheet should be placed under the detection area of ​​the cloth chip. Compared with traditional flow chemiluminescence, the cloth chip and detection method of the present invention do not need any expensive pump device (precision syringe pump, peristaltic pump, etc.) to drive liquid flow. The invention only drives the liquid flow through the natural gravity of the liquid and the capillary force of the cloth fiber gap.

Description

technical field [0001] The invention belongs to the field of microfluidic analysis, and relates to a cloth chip gravity / capillary flow chemiluminescence method, in particular to a cloth chip with gravity / capillary force coupling driving fluid and its application in detecting heavy metal ions. Background technique [0002] At present, Cr(VI), Cr(III), U(VI), Te(III), Co(III), Se(VI), Pu(III), Hg(II), Mn(VII) and other heavy metal ions The frequent occurrence of pollution incidents has brought enormous pressure to the natural environment and human life, so the rapid detection of heavy metal ions is very important in dealing with major emergencies and emergency incidents. [0003] In recent years, as an effective detection method, chemiluminescence has been widely used in the analysis of substances such as heavy metal ions, nucleic acids, and proteins. Chemiluminescence detection is mainly divided into static system, flow system and two-phase flow measurement system. Compared...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N21/76
Inventor 章春笋刘瑞
Owner SOUTH CHINA NORMAL UNIVERSITY
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