Near field enhanced Raman molecular fingerprint spectrum analysis method

A technology of near-field enhancement and Raman molecules, which is applied in the directions of Raman scattering and material excitation analysis, can solve the problems of difficulty in meeting requirements, inability to analyze in situ, and difficulty, and achieve a large detection dynamic range, cheap equipment, and Detect fast effects

Inactive Publication Date: 2006-09-27
DALIAN UNIV OF TECH
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) It is still difficult to analyze proteins with large and extra large molecular weights;
[0007] (2) Biomass spectrometry methods cannot distinguish isomeric protein molecules;
[0008] (3) It is impossible to maintain the activity of proteome biological samples, and it is not possible to analyze them in situ, and it is often necessary to disassemble the samples for analysis;
[0009] (4) The structural relationship between molecules in the proteome cannot be analyzed;
[0010] (5) The cont

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Near field enhanced Raman molecular fingerprint spectrum analysis method
  • Near field enhanced Raman molecular fingerprint spectrum analysis method
  • Near field enhanced Raman molecular fingerprint spectrum analysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0044] Specific embodiments of the present invention will be described in detail below in conjunction with technical solutions and accompanying drawings.

[0045] The present invention takes human serum samples as an example:

[0046] The near-field-enhanced Raman molecular fingerprints of the serum of people with and without hepatitis B virus should be different. The inventor once used a conventional micro-Raman spectrometer to experiment with the serum of people with and without hepatitis B virus, but no Raman spectrum results were obtained. . There are proteome samples in human serum without and without hepatitis B virus, and the method of near-field enhanced Raman molecular fingerprint analysis is implemented in the present invention, and the following steps are adopted:

[0047] label

HBsAg

(ng / ml)

HBsAb

(NCU / ml)

HBeAg

(mlU / ml)

HBeAb

(NCU / ml)

HBcAb(NCU / ml)

A9

0

0

0

0

0

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Mean scaleaaaaaaaaaa
Login to view more

Abstract

This invention relates to a near field enhanced Raman molecular fingerprint spectrum analysis method<0}, which comprises an optimized near-field reinforced Raman sample pond, an inverted or vertical microscope, and a Raman spectrometer. The analysis can comprise five steps or less according to request, which comprises mainly: preparing the sample into liquid for direct use, or taking full 2D gel electrophoretic separation; comparing the determined data with database. This invention special fit to active protein constituent analysis and medical research.

Description

technical field [0001] The invention relates to the technical field of biomacromolecule analysis, in particular to a proteomics near-field enhanced Raman molecular fingerprint analysis method. Background technique [0002] In spectroscopic analysis technology, molecular Raman scattering is due to the small scattering cross section (about 10 -29 cm 2 ) applications are very limited. In recent years, the surface-enhanced Raman scattering (SERS) technology can increase the Raman scattering cross section by many orders of magnitude, expanding the application range of Raman analysis. In 2002, Wu Shifa and Wu Guanying applied for the invention patent of "Raman scattering near-field enhanced sample cell with evanescent light for both excitation and reception" (application number: CN021544689). The application of this invention patent can greatly enhance the detection of "hot spots" in the sample cell. Electric field strength and increase the number of "hot spots" in the Raman an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/65
Inventor 吴世法刘琨李亚琴吴冠英张毅
Owner DALIAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap