Closed type bipolar electrode electrogenerated chemiluminescence fabric chip, and preparation method and application thereof

A bipolar electrode and chip technology, applied in the field of glucose, detection of tripropylamine, closed bipolar electrode electrochemiluminescent cloth chip, hydrogen peroxide, can solve the problems that the detection method and device have not been reported, and achieve the realization of Effects of high-throughput detection, reduced pollution, and ease of high-throughput detection

Inactive Publication Date: 2016-11-09
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the detection method and device combined with BPE-ECL and cloth chip have not been reported

Method used

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  • Closed type bipolar electrode electrogenerated chemiluminescence fabric chip, and preparation method and application thereof
  • Closed type bipolar electrode electrogenerated chemiluminescence fabric chip, and preparation method and application thereof
  • Closed type bipolar electrode electrogenerated chemiluminescence fabric chip, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] A method for preparing a closed amphoteric electrode electrochemiluminescence cloth chip, comprising the following steps:

[0066] (1) Use Adobe Illustrator CS5 drawing software to design the pattern of supporting / reporting microchannel, positive / negative driving electrode, and bisexual electrode, and then process it into a 300-mesh polyester microchannel screen (such as figure 1 As shown, the white area surrounds the microchannel) and the electrode grid (such as figure 2 As shown, the white is the electrode);

[0067] (2) Cotton pieces (white, cotton, ~10.7mg / cm 2 , ~96 wires / inch width) are placed under the electrode grid, so that the cloth and the grid are close to each other, and the driving electrode and bipolar electrode are brushed on the electrode grid by brushing conductive carbon paste (<60Ω / square). printed on cotton sheets;

[0068] (3) After painting, separate the cotton cloth sheet from the electrode grid, and bake it in an oven (model DHG-9035A) at 10...

Embodiment 2

[0078] The cloth chip that embodiment 1 makes is detected in the application of TPA, comprises the following steps:

[0079] (1) Fix the prepared cotton chip on a PET plastic support, and put a layer of gasket under the support (the part corresponding to the microchannel is hollowed out to make the microchannel suspend), on the one hand, it is convenient to fix the chip and keep it flat; on the other hand On the one hand, prevent the dropwise reaction solution in the supporting microchannel and reporting microchannel on the chip from contacting the stage, causing unnecessary pollution to the stage;

[0080] (2) Use the cut double-sided conductive tape and alligator clips to connect the positive drive electrode and negative drive electrode of the cotton chip on the bracket to the positive and negative electrodes of the DC power supply (model PS-305D), and put them together into the ECL On the stage of the measurement and analysis system; adjust the cloth chip position so that t...

Embodiment 3

[0086] Some important factors (driving voltage E tot , bipolar electrode width W BPE , the length of the bipolar electrode L BPE, bending angle θ) for optimization

[0087] a) Optimum driving voltage E tot

[0088] 1. Other conditions are the same as in Embodiment 2, that is, the cloth chip θ=0°, L wc =13mm, W wc = 6mm, L de = 8mm, W de =5mm,L BPE =15mm, W BPE =2mm, L ae =L ce =5mm; the volume of the test solution in the report microchannel is 15μL, Ru(bpy) 3 2+ The concentration is 5mM, and the TPA concentration is 25mM; the volume of PBS in the supporting microchannel is 15μL, and the concentration is 0.1M (pH 7.4).

[0089] 2. Set up several experimental groups: driving voltage E tot Set to several different values ​​(3V, 3.5V, 4V, 4.5V, 5V, 5.5V, 6V, 6.5V, 7V).

[0090] 3. The detection process of the cloth chip C-BPE-ECL is the same as in Example 2, and the test results are as follows Figure 5 shown.

[0091] It can be seen from the experimental results ...

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Abstract

The invention discloses a closed type bipolar electrode electrogenerated chemiluminescence fabric chip, and a preparation method and application thereof. The substrate of the chip is a fabric piece on which electrodes are distributed, a bipolar electrode is at the center, and a pair of drive electrodes are distributed at two sides; the positive drive electrode is communicated with the cathode of the bipolar electrode via a support microchannel, and the negative driving electrode is communicated with the anode of the bipolar electrode via a report microchannel; and a hydrophobic wax dam is arranged round the microchannels, and the microchannels are hydrophilic. The chip possesses the potential for preparing an ultra-flexible sensor because of extremely good flexibility. Additionally, compared with a conventional chip substrate material, the fabric chip possesses the advantages of cheapness, universality, friendliness to users, good biocompatibility, biodegradability and the like.

Description

technical field [0001] The invention belongs to the field of microfluidic chips, and in particular relates to a closed amphoteric electrode electrochemiluminescent cloth chip and its preparation method, and its application in detecting tripropylamine, hydrogen peroxide and glucose. Background technique [0002] Microfluidic chip, also known as micro-total analysis system, refers to an analysis system that uses micro-processing technology to form a micro-channel structure on a certain substrate material, which can be used for detection applications in the fields of biology, medicine, and chemistry. [0003] In 2011, the Dendukuri group introduced cotton cloth as a chip substrate material into the field of microfluidic chip processing for the first time, and used a loom to wind threads together to prepare a cloth-based microfluidic colorimetric immune chip. Compared with chips made of inorganic materials such as glass, silicon wafers, and quartz, and polymer materials such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/30
CPCG01N21/76G01N27/308
Inventor 章春笋刘敏
Owner SOUTH CHINA NORMAL UNIVERSITY
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