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Kit for detecting ubiquitin-binding enzyme antibody and use method thereof

A technology of ubiquitin-conjugated enzymes and kits, applied in the field of biomedicine, to achieve good clinical application value, high sensitivity and specificity, and easy operation

Pending Publication Date: 2021-07-20
SIR RUN RUN HOSPITAL NANJING MEDICAL UNIV
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Problems solved by technology

However, up to 15% of patients with clinically diagnosed T1DM are negative for all currently known islet autoantibodies

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  • Kit for detecting ubiquitin-binding enzyme antibody and use method thereof

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Embodiment Construction

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0026] In the description of this specification, descriptions with reference to the terms "one embodiment", "example", "specific example" and the like mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment of the present invention. In an embodiment or example. In this specification, schematic representations of the above terms do not necessarily refer ...

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Abstract

The invention discloses a kit for detecting a ubiquitin-binding enzyme antibody and a use method, and belongs to the field of biomedicine. The kit for detecting the ubiquitin-binding enzyme antibody comprises: a solution for detecting the ubiquitin-binding enzyme antibody; and the solution comprises an active ester for labeling a ubiquitin-binding enzyme antibody and a non-radioactive solution. The active ester comprises thioruthenium derivative active ester and biotin polyethylene glycol active ester. The kit further comprises a 96-well plate, a streptavidin graphene plate, a 0.01 mol / L PB, a 5% BSA solution and a PBST solution. The streptavidin graphene flat plate is an MSD96 pore plate marked by streptomycin. Compared with the prior art, the kit provided by the invention has the advantages of higher sensitivity and specificity, no use of radioactive substances, simplicity and convenience in operation, capability of detecting the amount of antibodies in trace serum, and very good clinical application value.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a kit for detecting ubiquitin-conjugating enzyme antibodies and a use method. Background technique [0002] Classical T1DM is an autoimmune disease that specifically damages islet β cells mediated by antigen-specific T cells. Islet autoantibodies play an important role in the prediction and diagnosis of T1DM. Combined detection of GADA, IAA, IA-2A and ZnT8A four islet autoantibodies can predict 85% or more of T1DM patients or T1DM high-risk groups. However, up to 15% of patients with clinically diagnosed T1DM are negative for all currently known islet autoantibodies. Therefore, discovering new islet autoantibodies or establishing a more accurate detection method is the main way to increase the number of autoantibody-positive T1DM patients. Aiming at the deficiencies of the prior art, a kit for detecting ubiquitin-conjugating enzyme antibodies and a use method are provided. Contents...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573G01N33/58
CPCG01N33/573G01N33/58G01N2800/24
Inventor 刘煜钱莉朱昱晓
Owner SIR RUN RUN HOSPITAL NANJING MEDICAL UNIV
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