Method for detecting adaptive evolution and structural modeling of rbcL genes of tamaricaceae plants

A technology of adaptive evolution and structural modeling, applied in biological systems, bioinformatics, special data processing applications, etc., can solve problems such as unknown functions and affecting enzyme catalytic efficiency

Inactive Publication Date: 2016-10-12
COLD & ARID REGIONS ENVIRONMENTAL & ENG RES INST CHINESE
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Problems solved by technology

Rubisco has a decisive influence on the net photosynthetic rate and is the key enzyme of photosynthetic carbon assimilation; Rubisco of all green plants is composed of 8 large subunits and 8 small subunits, and the large subunit is composed of wxya The composition of the gene code basically includes all the active sites required for enzyme catalysis, and the amino acid sequence of its catalytic center region determines the catalytic efficiency; the function of the small subunit is currently unknown, but it also affects the catalytic efficiency of the enzyme

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  • Method for detecting adaptive evolution and structural modeling of rbcL genes of tamaricaceae plants
  • Method for detecting adaptive evolution and structural modeling of rbcL genes of tamaricaceae plants
  • Method for detecting adaptive evolution and structural modeling of rbcL genes of tamaricaceae plants

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Embodiment Construction

[0026] The embodiments of the present invention are used to further illustrate the implementation of the present invention below in conjunction with the accompanying drawings, but the present invention is not limited thereto.

[0027] A kind of detection tamarixaceae plant wxya An overview of methods for genetically adaptive evolution and structural modeling:

[0028] The method comprises: ① Tamarix plants wxya Acquisition of gene sequences; ②Pedigree analysis and adaptive locus detection of 17 species of Tamarixaceae; ③Rubisco structure simulation and selective locus analysis of Tamarixaceae plants.

[0029] Among them ① Tamarixaceae plants wxya The steps to obtain the gene sequence are: log in to the NCBI platform and search for all Tamarix plants wxya Gene sequence; unreliable sequences and sequences without complete Rubisco catalytic active region were excluded and discarded wxya The sequence of the non-Rubisco catalytically active region of the gene; by entering a...

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Abstract

A method for detecting adaptive evolution and structural modeling of rbcL genes of tamaricaceae plants comprises steps as follows: acquisition of rbcL gene sequences of the tamaricaceae plants; pedigree analysis and adaptive site detection of 17 species of the tamaricaceae plants; Rubisco structural simulation and selective site analysis of the tamaricaceae plants. The rbcl genes of tamaricaceae covering three genera and 17 species are taken as objects, a branch-site model is taken as a model, the genes have three positive selection sites in the amino acid level according to detection, and the selective sits are remarkably related with hydrophobicity and entropy for maintaining protein and are also related with the concentration of global CO2. Besides, the adaptability of tamaricaceae plants to the drought environment is related with adaptive evolution of the rbcL genes.

Description

technical field [0001] The invention relates to a method for detecting Tamarix plants wxya Methods for Gene Adaptive Evolution and Structural Modeling. [0002] The present invention uses 3 genera and 17 species of Tamarixaceae rbcl Taking the gene as the object, the "branch-site" model was used to detect that the gene had three positive selection sites at the amino acid level; through Bioedit, it was deduced that these selective sites were not only significantly related to the hydrophobicity and entropy value of maintaining protein stability , and with the global CO 2 related to concentration changes. Background technique [0003] Ribulose-1,5-bisphosphate carboxylase / oxygenase (Rubisco, EC4.1.1.39) is considered to be the most abundant protein in the world. This enzyme has a dual function and can catalyze two reactions: one is Carboxylation reaction; Second, oxygenation reaction. Rubisco has a decisive influence on the net photosynthetic rate and is the key enzyme of...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/12
CPCG16B5/00
Inventor 燕霞马小飞钱朝菊
Owner COLD & ARID REGIONS ENVIRONMENTAL & ENG RES INST CHINESE
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