Method for detecting adaptive evolution and structural modeling of rbcL genes of tamaricaceae plants

Abstract

A method for detecting adaptive evolution and structural modeling of rbcL genes of tamaricaceae plants comprises steps as follows: acquisition of rbcL gene sequences of the tamaricaceae plants; pedigree analysis and adaptive site detection of 17 species of the tamaricaceae plants; Rubisco structural simulation and selective site analysis of the tamaricaceae plants. The rbcl genes of tamaricaceae covering three genera and 17 species are taken as objects, a branch-site model is taken as a model, the genes have three positive selection sites in the amino acid level according to detection, and the selective sits are remarkably related with hydrophobicity and entropy for maintaining protein and are also related with the concentration of global CO2. Besides, the adaptability of tamaricaceae plants to the drought environment is related with adaptive evolution of the rbcL genes.

Description

technical field [0001] The invention relates to a method for detecting Tamarix plants wxya Methods for Gene Adaptive Evolution and Structural Modeling. [0002] The present invention uses 3 genera and 17 species of Tamarixaceae rbcl Taking the gene as the object, the "branch-site" model was used to detect that the gene had three positive selection sites at the amino acid level; through Bioedit, it was deduced that these selective sites were not only significantly related to the hydrophobicity and entropy value of maintaining protein stability , and with the global CO 2 related to concentration changes. Background technique [0003] Ribulose-1,5-bisphosphate carboxylase / oxygenase (Rubisco, EC4.1.1.39) is considered to be the most abundant protein in the world. This enzyme has a dual function and can catalyze two reactions: one is Carboxylation reaction; Second, oxygenation reaction. Rubisco has a decisive influence on the net photosynthetic rate and is the key enzyme of...

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Problems solved by technology

Rubisco has a decisive influence on the net photosynthetic rate and is the key enzyme of photosynthetic carbon assimilation; Rubisco of all green plants is composed of 8 large subunits and 8 small subunits, and the large subunit is composed of wxya The composition of the gene code basically includes all the active sites required for enzyme catalysis, and the amino acid sequence of its catalytic center region determines the catalytic efficiency; the function of the small subunit is currently unknown, but it also affects the catalytic efficiency of the enzyme

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