Method for improving resistance of rice plant to cnaphalocrocis medinalis by transgenic technology

Abstract

The invention discloses a method for improving the resistance of rice plants to rice case worms by applying the transgenic technology, and belongs to the technical field of the plant gene engineering breeding. The method comprises the following steps: Rubisco specific promoters RubS and insect-resistant genes Cry1A(b) are constructed to a pPZP201 expression plasmid carrier; the Cry1A(b) genes are integrated into a rice genome by using the method of co-transformation mediated by agrobacterium tumefaciens; the homomycin resistance genes in the self-bred progenies are rejected. The Cry1A(b) insect-resistant genes in the transgenic rice present specific high-expression, present low-expression in seeds, and have no expression in roots, thus obviously improving the resistance of the rice plants to the rice case worms, greatly reducing the pesticide application amount, reducing the production cost and improving the plant profit; simultaneously, the expression of the insect-resistant protein in rice seeds, pollen and roots is decreased, and the worry of people to the rice security is reduced.

Description

technical field [0001] The invention relates to the technical field of plant insect-resistant breeding, in particular to a method for improving the resistance of rice plants to the rice leaf roller by applying biotransgenic technology. technical background [0002] Rice is one of the most important food crops in my country. The annual planting area accounts for about one-third of the crop planting area, and rice production accounts for about 40% of the total grain output. Therefore, high and stable rice production is of great significance to ensure food security. . According to the statistics of the National Agricultural Technology Center, in 2007, the rice leaf roller was violent and occurred heavily. It occurred in the Yangtze River Basin, Jianghuai, Southwest, South China and other rice areas. Serious threat; According to statistics from the Ministry of Agriculture, in 2007, the area of ​​rice leaf rollers controlled by the country reached 580 million mu times, an increas...

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Problems solved by technology

[0006] In order to effectively select the transformed cells, when carrying out genetic transformation, selectable marker genes such as Hpt and / or reporter genes such as Gus are used to select and identify the transformed cells, but after obtaining the transgenic plants, these marker genes and / or the reporter gene becomes redundant again

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