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Tobacco-derived protein compositions

A protein and composition technology, applied in the field of tobacco-derived protein composition, can solve the problems of incomplete combustion and pyrolysis products, no delivery, etc.

Active Publication Date: 2016-02-10
R J REYNOLDS TOBACCO COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, various ways have been proposed to provide many of the sensations of smoking, but none to deliver the considerable amount of incomplete combustion and pyrolysis products produced by burning tobacco

Method used

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  • Tobacco-derived protein compositions
  • Tobacco-derived protein compositions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119] Example 1: Exemplary provision of a RuBisCO-enriched extract by acidic clarification and precipitation

[0120] Tobacco plants are harvested, the leaves are stripped from the stems, and the leaves are homogenized in a pulverizer by adding water to them, and the material is then passed into a horizontal screw press for liquid extraction. The liquid protein-containing extract thus obtained was a green juice with a pH value of 8.6. This protein-containing extract was first clarified by passing the extract through a decanter. The pH of the protein-containing extract was adjusted to 5.9 using hydrochloric acid. Celite was optionally added to the extract and the mixture was stirred for 15 minutes then passed through a filter press.

[0121] The resulting clarified protein-containing extract was treated with citric and hydrochloric acid to adjust the pH to 4.92. The pH-adjusted extract was allowed to stand for 47 hours. The liquid was decanted from the top of the mixture...

Embodiment 2

[0122] Example 2: Exemplary provision of RuBisCO-enriched extracts by acid clarification

[0123] Tobacco plants are harvested, the leaves are stripped from the stems, and the leaves are homogenized in a pulverizer by adding water to them, and the material is then passed into a horizontal screw press for liquid extraction. The liquid protein-containing extract thus obtained was a green juice with a pH value of 8.4. This protein-containing extract was first clarified by passing the extract through a decanter. The pH of the protein-containing extract was adjusted to 5.9 using hydrochloric acid. Celite was optionally added to the extract and the mixture was stirred for 15 minutes then passed through a filter press. The filtrate was washed with water and the pH was adjusted to 5.9 to improve protein recovery.

[0124] The resulting clarified protein-containing extract was processed using tangential flow filtration on a 500 kDa reverse osmosis filter. The retentate was washed...

Embodiment 3

[0125] Example 3: Exemplary provision of a RuBisCO-enriched extract by acidic clarification and precipitation

[0126] Tobacco plants are harvested, the leaves are stripped from the stems, and the leaves are homogenized in a pulverizer by adding water to them, and the material is then passed into a horizontal screw press for liquid extraction. The liquid protein-containing extract thus obtained was a green juice with a pH value of 8.7. This protein-containing extract was first clarified by passing the extract through a decanter. The pH of the protein-containing extract was adjusted to 5.9 using hydrochloric acid. Celite was added to the extract and the mixture was stirred for 15 minutes then passed through a filter press.

[0127] The resulting clarified protein-containing extract was treated with hydrochloric acid to adjust the pH to 4.98. The pH-adjusted extract was allowed to stand for 60 hours. The liquid was decanted from the top of the mixture and a settled solid w...

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PUM

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Abstract

The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of one or more of ash, metal salts, alkaloids, particulates, heavy metals, and other impurities and / or contaminants from extracts, as well as modifying the sensory characteristics (e.g., odor, color, and / or taste characteristics) of extracts. The methods generally include diafiltration, treatment with functionalized resins, and supercritical extraction. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant of the Nicotiana species, wherein the protein composition is characterized by one or more of: an ash content of less than about 15% by weight; a nicotine content of less than about 10 [mu]g / g; and a heavy metal content of less than about 60 [mu]g / g.

Description

technical field [0001] The present invention relates generally to products made or derived from or otherwise containing tobacco or components of tobacco and intended for human consumption. Of particular interest are ingredients or components obtained or derived from plants or plant parts of the Nicotiana species. Background technique [0002] Many uses of tobacco have been proposed. For example, tobacco has been smoked in pipes, and tobacco has been used for smoking in cigarettes and cigars. See, eg, Tobacco Production, Chemistry and Technology, Davis et al. (eds.) p. 346 (1999). In recent years, various ways have been proposed to provide many of the sensations of smoking, but not to deliver the considerable amount of incomplete combustion and pyrolysis products produced by burning tobacco. See, for example, the background art described in U.S. Patent Nos. 7,503,330 to Borschke et al. and 7,726,320 to Robinson et al., and U.S. Patent Application Serial Nos. 13 / 840,264 to ...

Claims

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Application Information

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IPC IPC(8): A24B15/24A23J1/00A23J3/14A24B15/18A23L5/20A23L33/17A23L2/66C07K14/415A23K20/147
CPCA24B15/18A24B15/24C12Y401/01039A23K20/147A23L5/23A23L5/273A23L33/185C07K14/415A23J3/14A23L2/66A23J1/007A24B15/20A24B15/241C12N9/88A23V2002/00
Inventor A·R·杰拉尔迪C·D·H·伯德T·J·杰克逊C·A·贝茨J-P·穆阿K·弗德
Owner R J REYNOLDS TOBACCO COMPANY
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