34 results about "Flower Filaments" patented technology

The invention relates to a processing method of yellow chrysanthemum tea. The processing method comprises the following steps: 1, picking fresh flowers; 2, spreading for placing in a grading manner; 3, cleaning the flowers; 4, primarily drying; 5, secondarily drying; 6, full firing; and 7, stalk-separating and grading. The processing method has the beneficial effects that the yellow chrysanthemum tea in a dry state is gold-yellow in filament and green in receptacle; whole chrysanthemum is suspended in a cup after the yellow chrysanthemum tea is made, and is gold-yellow; the yellow chrysanthemum tea is gold-yellow and bright in soup color, clean and durable in aroma and mellow in taste. According to the processing method, a high-temperature green-removing (flower steaming) process of conventional chrysanthemum tea is changed in a processing technique and a spreading process is adopted, and thus the processing time is prolonged, the transformation of internal ingredients of the chrysanthemum is promoted, a multi-time section drying method is adopted in the drying procedure, the temperature is gradually increased, the dissipation of water and the collation of aroma substances are facilitated; by applying a relatively-high-temperature full firing process, low-melting point aroma substances relatively quickly volatilize, the flower aroma is relatively pure, and the aroma volatilizes relatively remarkably after the yellow chrysanthemum tea is made.

The invention relates to the method for identifying the varieties of lilium brownii by adopting an SSR (simple sequence repeat) molecular marking technology. A result shows that: two pairs of high-polymorphism and high-repeatability primers are screened from 13 pairs of primers for detecting 171 fragments comprising 131 polymorphic fragments, 4 to 22 fragments can be detected by each pair of primers with the sizes of 100 to 250bp, 1.69 bands are amplified by each pair of primers, and the polymorphism rate is 76.70 percent; NTSYS-pc2.11 is used for similarity coefficient and cluster analysis, the genetic similarity coefficient is 0.5882 to 09412, and the average similarity coefficient is 0.7647; materials are divided into six major classes by cluster analysis, the genetic differences between 11 lilium brownii germplasms are irrelevant to varietal characteristics, flower forms, the colors of filaments and styluses, but have certain correlation with the colors of petals, flower genes can better reflect genetic relationships between the lilium brownii germplasms, different varieties have different colors, and the primers can be designed by controlling related genes of flowers to provide an important means for further distinguishing the genetic relationships.

The invention relates to a making method of a Camellia magniflora filament chromosome. The method includes the steps of: selecting potted Camellia magniflora of 5-10 years old and putting it in a greenhouse, at 9-10a.m. in a sunny day, and taking filaments from vigorously growing potted Camellia magniflora flower buds; rinsing the obtained filaments with ice water, then putting the filaments immediately into a pretreatment solution and performing treatment in a 4DEG C refrigerator for 8-10h; rinsing the pretreated filaments, then performing fixation with stationary liquid for 24h; then conducting 55DEG C water bath dissociation in a dissociation liquid for 10-12min; and then dyeing the dissociated filaments with carbol fuchsin dyeing agent for 10min, and carrying out microscopic examination photographing and karyotype analysis. The method provided by the invention can accurately and quickly determine the ploidy of Camellia magniflora and acquire the clear images of a variety of split phase chromosomes, thus overcoming the technical problem that because of a lot of Camellia magniflora chromosomes, a clear dissociation phase can be difficultly acquirable, and laying a good foundationfor systematic research of the configuration change rule of Camellia magniflora chromosomes, variety improvement and crossbreeding.

A method for detasseling Compositae plants is characterized in adopting artificial detasseling through cutting corolla in plant breeding of Lactuca, Sonchus, and Taraxacum of Compositae. the method comprises cutting maternal calyces to remove corolla in early morning before florescence without injuring calyces, after 1 h, pistil grows out new thrumb, chapiter and petal, when the chapiter grows to Y shape, pollinating with male parent through cap bag at once to perform intergeneric crossing. Compared with present technology, the invention creates conditions for hybridization breeding of Lactuca, Sonchus, and taraxacum of Compositae due to the regrowth vigor and recuperability of their corolla, thrumb, and chapiter, and promotes the development of conventional breeding technology.

The invention belongs to the technical field of hybridization methods or devices for mung beans, and discloses a high-efficiency cross breeding method of mung beans. The high-efficiency cross breedingmethod comprises the following steps of, three days before hybridization work, spraying brassinolide with a concentration of 50 milligrams per liter and boric acid with a concentration of 1,000 milligrams per liter; performing the emasculation of a female parent: in the afternoon of the day before pollination, selecting an appropriate flower bud of the female parent, separating a vexillum and anala by using the tips of tweezers, longitudinally splitting a carina, removing 10 stamens from the base of a filament, completely removing other large and medium flower buds and already borne pods, which belong to the same flower cluster with an emasculated flower, and hanging a board on a pedicel; performing the pollination: sucking 200 microliters of mung-bean pollen suspension mixed liquor by using a pipette, and injecting 10 to 20 microliters of the mung-bean pollen suspension mixed liquor on a stigma of the female parent each time; and clearly writing a male parent, the female parent andhybridization time on the board hung on the pedicel. By using the high-efficiency cross breeding method, the work efficiency is increased by 225%, and the hybridization success rate is increased by 17.4%.

The invention provides a method for feeding sesamia inferens by means of tender corn ears. According to the method, tender corn ears with bracts and filaments in the silking stage to grain filling stage is inoculated with nigrescent egg mass of sesamia inferens, and the corn ears are replaced with fresh corn ears 8-10 days later till larva pupation; during pupation, paper rolls are placed in a feeding box to provide space for pupation; after adult emergence, adults are placed in a laying coop with tillering-stage rice seedlings inside for oviposition, and eggs with leaf sheath are taken out, sterilized with formaldehyde of 10%, and then placed in a culture dish with degreasing cotton and wet filter paper inside for moisturizing till the egg mass develops to become black. By feeding sesamia inferens by means of tender corn ears in the silking stage to grain filling stage, the survival rate and development rate of early larvae, pupal weight and the fertility of adults are increased remarkably. The method is suitable for large-scale breeding of sesamia inferens standard test insects, and provides technical support for obtaining sufficient and high-quality insect sources.

The invention discloses a cleistogamic mutant material of brassica napus. The cleistogamic mutant material of the brassica napus provided by the invention is a cleistogamic mutant Zhong 9-Clg, and isobtained by screening of brassica napus cultivar Zhongshuang No. 9 subjected to induced mutation by ethyl methyl sulfone; and the mutant shows that petals are closed without cracking during the entireflowering period, but fruiting is normal, the petals are shrunken, the edges of calyxes are adherent, no missing or obvious structural variation is found in other flower organs such as stigmas, filaments and anthers, electron microscope scanning results show that the epidermal cells of the petals of the mutant have irregular arrangement, cuticles are reduced, the number of stomata in the lower surfaces of the calyxes is reduced, the cell ornamentation is in the shape of stripes, the upper epidermal cells are disordered and wrinkles exist. The pollination traits of the cleistogamic mutant Zhong 9-Clg are utilized to change the pollination behavior of the brassica napus, so that a plurality of excellent traits such as excellent brassica napus quality can be maintained stably, and theoretical and technical support is provided for breeding of the brassica napus.

The invention discloses a production process of maize flower tea, which mainly includes the steps of selecting raw materials, finishing and separating the raw materials, cutting the raw materials, withering the maize flowers, deactivating enzyme, rolling the maize flowers, adding the liquid generated during the rolling step, loosening the maize flowers, fermenting the maize flowers, drying the maize flowers to enhance fragrance, and classifying and packaging finish products. In the method, sweet corns or baby corns are selected; the maize flower tea produced according to the steps not only hasexcellent healthcare functions, but also has the refreshing taste of corn and gentle and delicate taste. The production process also solve problems of flower filament production at low cost and highyield, so that the process has excellent application prospect.

The invention discloses a production process of corn stigma tea. The production process of the corn stigma tea mainly comprises the following steps: raw material selection, stigma collection, cutting, withering, rolling, soup returning and scattering, fermentation, drying fragrance extraction, and grade packaging of finished products. Through breeding a large-flower variety of corn, the corn stigma tea processed through the steps and the process not only has rich health-care effects, but also has pure taste, faint scent and sweet aftertaste, and further achieves low-cost and high-output stigma production, thereby having good application prospects.

The invention discloses a self-adaptive carthamus tinctorius flower ball gathering device. The self-adaptive carthamus tinctorius flower ball gathering device comprises a machine frame part, a combingbaffle part and a combing-clamping-type picking head part. The machine frame part is used for fixing each part. A combing device is installed at the front end of a machine frame. The combing-clamping-type picking head part is installed above a baffle at the side edge of the combing device. The combing baffle part comprises the baffle at the side edge of the combing device and a spring. In harvesting operation, a flower ball is transitorily fixed under the action of a floral axis and the baffle at the side edge of the combing device, after picking by a combing-clamping-type picking head, the device is continuously moved forwards, an acting force of the floral axis to the flower ball is increased, the spring is compressed, and a gap between the neighboring baffles at the side edge of the combing device is increased, so the picked flower ball is fallen from the gap, and a picking process is completed. The device is capable of, through using the spring and the baffles at the side edge ofthe combing device, realizing self-adaptive fixing to the flower ball, effectively solving problems that joggling of the flower ball is serious and feeding of a flower filament is difficult, reducinga damage rate of the flower ball, and improving a complete picking rate of the flower filaments.

The invention provides a method for obtaining a pure line through inducing parthenogenesis of Zea mays by a chemical agent. The method comprises the following steps: (1) preparing the chemical agent; (2) carrying out induction treatment: completely withdrawing tassels of plants required to be treated when the tassels appear before stamen stalks are drawn, strictly bagging female ear flowers, after the stamen stalks are drawn for 1 to 3 days, and scissoring each stamen stalk drawn portion into a notch by a pair of disinfected scissors in early morning of the same day of treatment before dew is evaporated; putting an induction agent dipped absorbent cotton ball at each notch, not taking off absorbent cotton balls so as to enable the absorbent cotton balls to be in full contact with the stamen stalks, and finally bagging paper bags again; and in the morning of the next day, injecting the induction agent to the stamen stalks without uncovering pollination bags, so as to carry out secondary induction; (3) carrying out harvesting; and (4) carrying out progeny testing. According to the method provided by the invention, the contamination caused by foreign pollen during the induction of the chemical agent is avoided, the setting percentage of induction and the number of grains of each ear are increased, and the aims of rapidly breeding the pure line and carrying out large-scale application are achieved.

The invention discloses an environment-friendly method for preventing corn female ear bags from falling off. The method is characterized by including the following steps that when female ears expand and before filaments are exposed out of bracts, 45-degree angles are formed through bending in the horizontal direction of leaf surfaces and at the positions, 10 cm away from leaf sheaths of stalks, of corn cob position leaves, and sheep skin paper bags 18 cm in length and 11 cm in width are wholly put in the corn cob position leaves and the female ears in a sleeving mode and embedded into the bonding positions of the leaf sheaths and the stalks. According to the method, the 45-degree angles are formed through bending in the horizontal direction of the leaf surfaces and at the positions, 10 cm away from the leaf sheaths of the stalks, of the corn cob position leaves, then the sheep skin paper bags are wholly put in the corn cob position leaves and the female ears in the sleeving mode, the sheep skin paper bags are supported through the corn cob position leaves, the bags are fixed by themselves in the mode that the female ears and the bent portions of the corn cob position leaves naturally grow to form expansion resistance with the sheep skin paper bags, the problem that the bags fall off in the bagging period is solved, and the influence of reduction, caused by breaking of the corn cob position leaves, of the selfing line hundred-grain weight is avoided.

The invention belongs to the technical field of corn breeding, and in particular relates to a method for producing corn mutants through EMS mutagenesis. Including pollen collection, pollen mutagenesis treatment and artificial pollination steps, wherein the collected pollen is added to the EMS-paraffin oil solution after the anthers are removed, and the mutagenesis is carried out according to the following steps: (1) irradiate under the ultraviolet lamp for 30-60s, (2) Stir in the dark for 20-30 minutes, (3) irradiate under the ultraviolet lamp for 15-30 seconds, (4) stir in the dark for 15-20 minutes; before artificial pollination, apply EMS‑on the ear filaments of the corn plants to be pollinated paraffin oil solution to obtain mutagenized filaments, and then artificial pollination. The invention utilizes the different mutagenesis mechanisms of ultraviolet radiation and EMS, combines the two mutagenesis methods, and has good versatility, and also performs mutagenesis treatment on filaments to cause mutations in some genes in the pistil, with a high mutation rate.

The invention relates to a water-saving and efficient cultivation method for red flowers utilizing drip irrigation, pertaining to the technical field of cultivation of medicinal plant. The method comprises following steps: variety selection, soil selection, soil preparation and fertilizer application, seed processing, proper sowing, reasonable close planting, thinning and final singling, water saving and irrigation, pest and diseases prevention and control, and harvesting and processing and comprehensive utilization of many technologies. The water-saving and efficient cultivation method for red flowers utilizing drip irrigation has following beneficial effects: the method is utilized for cultivation of red flowers so that water irrigation amount per mu is 70-100 m<2>; compared with the conventional cultivation, water can be saved by 440 <2>; yield per mu of red flower filaments can be up to 30-35 kg and red flower seeds per mu is 135 kg; compared with conventional cultivation per mu, the yield of filaments is increased by 15 kg; and the red flower seeds per mu is increased by 35 kg; the flower filaments is sold by 90yuan / kg; the red flower seeds is sold by 50 and yield per mu is increased by 1525 yuan; regardless of cost of 500 yuan / mu for drip irrigation equipment and water cost of 100 yuan, additional net profit per mu is 975 yuan.

The invention relates to a preparation method of functional compounding scented tea capable of warming the uteruses and promoting pregnancy and the compounding scented tea prepared by the method. The functional compounding scented tea is prepared through the following steps of cleaning rose flowers and pagodatree flowers, and drying the cleaned rose flowers and the cleaned pagodatree flowers; cleaning lily bulbs and seville orange flowers, shredding the cleaned lily bulbs and the cleaned seville orange flowers, and performing drying so as to obtain shredded lily bulbs and shredded seville orange flowers; performing cleaning on red jujubes, ginger, radix codonopsis, glossy privet fruits, rhizoma polygonati odorati and the like, performing drying, performing crushing so as to obtain red jujube powder, ginger powder, radix codonopsis powder, glossy privet fruit powder and rhizoma polygonati odorati powder, putting the red jujube powder, the ginger powder, the radix codonopsis powder, the glossy privet fruit powder and the rhizoma polygonati odorati powder in a closed container, performing high-pressure cooking, and then performing filtration so as to obtain first filtrate and first filter residues; putting the first filter residues and black tea in the closed container, performing high-pressure cooking, and then performing filtration so as to obtain second filtrate and second filter residues; mixing the first filtrate with the second filtrate so as to obtain a mixture, performing vacuum drying and performing crushing; and mixing the crushed mixture with the shredded lily bulbs, the shredded seville orange flowers, the cleaned and dried rose flowers and the cleaned and dried pagodatree flowers so as to prepare the functional compounding scented tea capable of warming the uteruses and promoting pregnancy. The prepared functional compounding scented tea capable of warming the uteruses and promoting pregnancy has the beneficial effects that the functional compounding scented tea has a good function of promoting pregnancy of females, and besides, components with fragrant smell exist, so that the compounding scented tea is fragrant and mellow in taste and easy to drink in mouth.

The invention relates to a gathering type red flower picking mechanism which comprises a stepping motor, a ball screw sliding table, moveable teeth, a moveable chassis, a collecting box, a square fruit ball channel and a frame. The working process is as follows: when the red flowers are gathered into five straight lines in the square fruit ball channel, the moveable teeth respectively fixedly arranged on the ball screw sliding table are controlled by the corresponding stepping motor to move relative to each other so as to tightly clamp the filaments, the moveable chassis fixedly arranged on the ball screw sliding table moves upward under the control of the corresponding stepping motor, the filaments are separated from the fruit ball, the moveable teeth are controlled by the corresponding stepping motor to have the same motion so as to send the filaments to an appointed position under the state of the filaments clamped by the moveable teeth, the moveable teeth are controlled to separate by the stepping motor and the filaments fall into the collecting box so as to complete the collection, and meanwhile, the moveable chassis is controlled to fall onto an original position by the stepping motor, the moveable chassis is controlled to return to an initial position by the corresponding stepping motor and the new red flower harvesting work can be started. According to the invention, the picking for the red flower filaments is converted from point-to-point picking to blind picking, the working efficiency is increased and the production cost is saved.

The invention discloses a method for preventing selfing by performing artificial emasculation on witloof and increasing the cross-pollination hybridizing rate of the witloof. The witloof is in a full-bloom stage in the middle of June, inflorescence which is healthy, does not have plant diseases and insect pests and has anther not bloomed is selected at about 8 o'clock in the morning of cloudless and windless weather, a pointed tweezers move from bottom to top along the gap between filaments of a flower base, the gathered anther walls are torn, pollen is not touched in the operating process, the anther at the top of a stamen is removed, the anther clings to a stylet, the stylet is washed by a clean water-containing small bottle with a spraying device to prevent selfing, a pencil with an erasehead is used for dipping the pollen of different varieties of witloof to coat the stylet head, and the whole process of cross-pollination is completed. The operating method is simple, damage to petal and pistil is avoided, the selfing maturing rate of the witloof can be effectively reduced, the cross-pollination hybridizing rate of the witloof is obviously increased and the success rate of artificial cross breeding is increased.

The invention discloses a method for evaluating the resistance of strawberry stamens to botrytis cinerea. The steps are: A. picking off the flower and the flower stalk from the plant when the strawberry flower just begins to bud; B. inserting the flower in the solidified water In agar, in vitro culture under light conditions; C, use tweezers to pick off the petals from the flower; D, stick the conidia suspension of Botrytis cinerea with a brush, and apply the conidia to the anthers of the stamens for inoculation; Inoculate the botrytis cinerea mycelium block on the PDA medium; E, reinsert the flower in solidified water agar, and place it under constant temperature and light conditions for in vitro culture; F, remove the young strawberry fruit from the sepals; G 1. Measure the lesion length on the filament, grade the disease index of each stamen respectively; H, calculate the disease index of each variety respectively; 1, the disease index of each variety classify its botrytis cinerea resistance. The method is easy to operate and the operation is simple and convenient. This method can quickly identify the resistance to Botrytis cinerea in the sepals of strawberry cultivars.

The invention provides a kiwi pollen mixture as well as a preparation method and application thereof. The mixture comprises the following ingredients in parts by weight: 6 to 8 parts of kiwi male flower filament powder, 3 to 5 parts of pedal powder, 8 to 10 parts of calyx powder and 1 to 2 parts of kiwi male flower pollen. The preparation method of the mixture comprises the following steps: (1) respectively drying filaments, pedals and calyx of kiwi male flowers for more than 12 hours at the temperature of 40 to 70 DEG C; (2) respectively sieving the dried filaments, pedals and calyx by utilizing a 200-mesh sieve, and collecting the sieved powder to respectively obtain the filament powder, the pedal powder and the calyx powder; and (3) uniformly mixing 6 to 8 parts by weight of filament powder, 3 to 5 parts by weight of pedal powder, 8 to 10 parts by weight of calyx powder and 1 to 2 parts by weight of kiwi male flower pollen obtained in the step (2), thus obtaining the kiwi pollen mixture. The mixture can be applied to artificial pollination and has advantages of low cost, high fruit setting ratio and high average weight of a single fruit.

The invention provides a method for feeding sesamia inferens by means of tender corn ears. According to the method, tender corn ears with bracts and filaments in the silking stage to grain filling stage is inoculated with nigrescent egg mass of sesamia inferens, and the corn ears are replaced with fresh corn ears 8-10 days later till larva pupation; during pupation, paper rolls are placed in a feeding box to provide space for pupation; after adult emergence, adults are placed in a laying coop with tillering-stage rice seedlings inside for oviposition, and eggs with leaf sheath are taken out, sterilized with formaldehyde of 10%, and then placed in a culture dish with degreasing cotton and wet filter paper inside for moisturizing till the egg mass develops to become black. By feeding sesamia inferens by means of tender corn ears in the silking stage to grain filling stage, the survival rate and development rate of early larvae, pupal weight and the fertility of adults are increased remarkably. The method is suitable for large-scale breeding of sesamia inferens standard test insects, and provides technical support for obtaining sufficient and high-quality insect sources.

The invention provides a method for cultivating red-pulp hylocereus polyrhizus. The method includes the steps that anthracnose-free hybridized target single plants with the features that the numbers of borne fruits are large in successive years, the weight of single fruit bodies is 400 g or above, the fruit bodies are elliptic, fresh, sweet, elegantly fragrant and good in taste, pulp is bright and rosy, and branches are thick and solid are marked; particularly, branches of the plants in which pistil protrudes out of stamen thrums by 1 cm or less are preferentially and independently collected, sterilized, detoxified and subjected to seedling growing, seedlings are transplanted to land, large-area fruit tree management is carried out, and commercial hylocereus polyrhizus is formed. After three-year intensive-culturing test application, the yield can be continuously high, and the fruits are large and are stabilized to be 0.4-0.5 kg or above; compared with the earlier stage, the fruits are fresher, sweeter and more elegantly fragrant, the shapes of the fruits are not varied and kept elliptic, the content of fructose is about 6.8%, the content of glucose is about 4.2%, and the content of haematochrome is about 4%; other excellent substance characters such as nutrients, the health caring function and the medicine function are not changed, the characters of the original male parent are kept, the hybrid vigor is remarkably expressed, the survival rate is 97% or above, the fruit bearing rate is 95% or above, and the red-pulp rate is 95% or above.

The invention discloses application of GT1 and HB13 genes in regulation and control of non-degeneration and lodging resistance of corn female ear lower flowers. The method comprises the following steps: carrying out gene editing on two targets, namely GT1 and HB13 in corn by utilizing a CRISPR / Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR associated 9) technology; the result shows that compared with a wild type and two single-process materials, the phenotype of the gt1 / hb13 double-mutant plant is more violent, the tiller number is obviously increased, the root system is more developed, the cluster number is increased, new female ears grow on ear stems of main ears, multi-ear property is shown, axillary meristem at the lower part is developed into female ears without inhibition, lower flowers in the female ears are not degraded, and the yield of the gt1 / hb13 double-mutant plant is increased. After the GT1 and HB13 genes are knocked out, the heart skin of the inferior flowers continuously develops to form filaments, some inferior flowers can also form two filaments, the phenomenon of multiple filaments is shown, the inferior flowers continuously develop to form extra small grains in the later period, and it is shown that after the GT1 and HB13 genes are knocked out, a corn mutant plant can show strong female spike inferior flower non-degeneration phenotype and lodging resistance.

The invention discloses a cytoplasmic male sterility production method for fresh corn silk. The method selects corn hybrids with thick filament, large filamentous scale and high yield and converts the corn hybrids into sterile hybrids; an isolation area is selected by adopting a time-space isolation mode; the selected male-sterile hybrids and fertile corn hybrids are sown pro rata by stages; after filaments of the early-sown male-sterile hybrids burst into bract for 5 to 10 days, the fresh corn silk is harvested on plants of the male-sterile hybrids; and after the corn silk is harvested on the male-sterile hybrids, pollen allowing the male-sterile hybrids to normally fruit is supplied by male flowers of the late-sown fertile corn hybrids. The method has the advantages that the method can produce the fresh corn silk with good quality and high yield, and reduce the production cost and labor input for the corn silk.

The invention discloses a swinging-type cutting device for flos carthami. The swinging-type cutting device comprises a rack, a roll-clamping device, a cutting device body and a collecting device. According to the working principle of the swinging-type cutting device, flower filaments enter a sealing box through circular holes, flower balls are stopped outside the sealing box, the flower filamentsenter two rubber rollers which rotate oppositely under the effect of a draught fan, and through a gerotor motor, a cutter is driven to separate the flower filaments from the flower balls. Then throughsuction force generated by the draught fan, the flower filaments are all sucked into a container through a conveying pipeline. The cutting device is simple in structure and low in cost, the flos carthami can be harvested on a large area, the labor intensity is reduced, the harvesting efficiency is improved, and the labor is saved.

The invention provides a method for collecting tobacco anther and pollinating, and belongs to the technical field of crop breeding. The method for collecting the tobacco anther comprises the following steps: stamens of flowers are distributed among comb teeth of an anther auxiliary stripper, the flowers are pulled in the direction opposite to the growth direction of the stamens, and the comb teeth are used for stripping the anther on filaments; the anther auxiliary stripper comprises a bottom support and the comb teeth fixed to the bottom support, and the distance between every two adjacent comb teeth ranges from 0.8 mm to 1.2 mm. According to the method, the male parent anther is collected through the special anther auxiliary stripper, and the pollen is aired and dispersed, so that the pollen collection efficiency can be obviously improved, the output and consistency of the pollen are ensured, and subsequent hybrid seed production and pollination are facilitated.

The invention discloses a method for obtaining regeneration plants of petunia hybrida by anther culture. The method comprises the following steps of: A) performing pretreatment and disinfecting petunia hybrida buds at low temperature; B) peeling calyx and petals of the buds obtained in the step A) by using tweezers, extracting anther, and after removing capillament of the anther completely, inoculating the anther to an inducing callus culture medium; C) transferring callus obtained by subculture in the step B) to a differential culture medium to differentiate adventitious buds, and when the adventitious buds are between 3 and 5 centimeters, stopping differential culture; and D) transferring the adventitious buds which are obtained in the step C) and are used as plantlets to a rooting culture medium to perform rooting culture until at least three adventitious roots of which the lengths are more than or equal to 2 centimeters grow on the plantlets to obtain seedlings which can be planted outside a bottle, wherein the steps A), B), C) and D) are performed in sterile environment.

The invention relates to a method for the artificial crossing of jerusalem artichoke. The method is to carry out a stamen removal operation on the inflorescence of a feverfew in the following florescence stage: the tubular flower at the peripheral edge of the flower disc has the following structure: the stamen is a syngenesious one which comprises filaments and anthers, wherein the filaments are separated, the anthers inosculate with each other to form a tubular shape, and the anthers do not shed pollen; the pistil comprises a stigma, a style and an ovary, wherein the style and the stigma arepositioned in the center of the bottom in the anther, the style is not extended, and the distance between the stigma and the top end of the anther is 2mm-3mm; and the top end of the anther is the endaway from the flower disc. The method is convenient and effective, well avoids the complication of continuous stamen removal and pollution to pollen, can prevent idiogamy, obtains a high-purity hybrid seed, and ensures the accuracy and reliability of the hybrid seed pedigree. The method is also applicable to the artificial crossing of other crops with capitula in the feverfew. The method providesa new technical measure for the germ plasm resource innovation of jerusalem artichoke, lays a solid foundation for the new germ plasm resource development of jerusalem artichoke, and has wide application prospects in the selective breeding of jerusalem artichoke hybrid varieties.

The invention belongs to the technical field of agricultural breeding production. Aiming at the problems of strict storage conditions and relatively high cost in a corn pollen low-temperature preservation technology, the invention provides a corn pollen storage method, which comprises the following steps of: 1, extracting tassels which just begin to loosen pollen from the top end of a corn plant along with a section of stalk; step 2, putting the taken-down tassel stalks into a container filled with water, binding the tassels by using a preservative film, putting the tassels into a refrigeration house, and preserving the tassels in a dark place; step 3, taking out the tassels from the refrigeration house 6-18 hours ahead of time according to the drawing time of the female tassels, placing the tassels at room temperature, and when pollen is taken, firstly placing a pollen collection container, then unwrapping the preservative film, lightly beating the tassels, and collecting pollen with cracked tassel anther into the container; 4, the collected pollen is sent to the field in time, and corn ear filament pollination is completed. The method is simple to operate and high in pollen extraction efficiency; the storage conditions are simple, and the storage cost is saved.