The invention belongs to the technical field of agricultural breeding production. Aiming at the problems of strict storage conditions and relatively high cost in a corn pollen low-temperature preservation technology, the invention provides a corn pollen storage method, which comprises the following steps of: 1, extracting tassels which just begin to loosen pollen from the top end of a corn plant along with a section of stalk; step 2, putting the taken-down tassel stalks into a container filled with water, binding the tassels by using a preservative film, putting the tassels into a refrigeration house, and preserving the tassels in a dark place; step 3, taking out the tassels from the refrigeration house 6-18 hours ahead of time according to the drawing time of the female tassels, placing the tassels at room temperature, and when pollen is taken, firstly placing a pollen collection container, then unwrapping the preservative film, lightly beating the tassels, and collecting pollen with cracked tassel anther into the container; 4, the collected pollen is sent to the field in time, and corn ear filament pollination is completed. The method is simple to operate and high in pollen extraction efficiency; the storage conditions are simple, and the storage cost is saved.