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Making method of Camellia magniflora filament chromosome

A technology of chromosome preparation and camellia, applied in the field of plant cytology research, can solve the problems of large flowering, poor resistance, small flower diameter, etc.

Inactive Publication Date: 2018-01-26
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The red camellia group (Sect. Camellia) has a large number of flowers, but the flower diameter is small and the resistance is poor

Method used

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  • Making method of Camellia magniflora filament chromosome
  • Making method of Camellia magniflora filament chromosome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 successfully observed and counted the number of chromosomes of the single-petal large-flowered camellia.

[0025] 1) Material treatment: From November to December, place the 5-10-year-old potted Camellia japonica in the greenhouse and water it thoroughly;

[0026] 2) Material collection: After watering thoroughly for 3 to 5 days, at 9 to 10 am on a sunny day, take the vigorously growing potted Camellia japonica flower buds, remove the outer bud scales, young petals, and ovaries, and only leave the stamens;

[0027] 3) Pretreatment: After rinsing the obtained stamens with ice water, immediately put them into the pretreatment solution of 10mg / L 8-hydroxyquinoline + 500mg / L essential oil, and treat them in the refrigerator at 4°C for 8-10h;

[0028] 4) Fixation: Rinse the pretreated stamens with distilled water for 3 times, and then put them in fixative solution (absolute ethanol: glacial acetic acid = 5:2) for 24 hours;

[0029] 5) Dissociation: Rinse the fixed...

Embodiment 2

[0035] Example 2 Successfully observed and counted the number of chromosomes in the double-petaled Dahuahong camellia

[0036] Concrete implementation method is the same as embodiment 1, microscopic examination result and photomicrograph see figure 2 .

[0037] The above results of implementation and application on two representative varieties of Camellia daflora show that this method is an effective method for preparing chromosomes of Camellia daflora, and the method for making chromosomes is simple and convenient to operate, and the chromosomes are evenly dispersed and the background is light. The number of chromosomes is easy to count, and the number of chromosomes obtained by this method has high accuracy.

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Abstract

The invention relates to a making method of a Camellia magniflora filament chromosome. The method includes the steps of: selecting potted Camellia magniflora of 5-10 years old and putting it in a greenhouse, at 9-10a.m. in a sunny day, and taking filaments from vigorously growing potted Camellia magniflora flower buds; rinsing the obtained filaments with ice water, then putting the filaments immediately into a pretreatment solution and performing treatment in a 4DEG C refrigerator for 8-10h; rinsing the pretreated filaments, then performing fixation with stationary liquid for 24h; then conducting 55DEG C water bath dissociation in a dissociation liquid for 10-12min; and then dyeing the dissociated filaments with carbol fuchsin dyeing agent for 10min, and carrying out microscopic examination photographing and karyotype analysis. The method provided by the invention can accurately and quickly determine the ploidy of Camellia magniflora and acquire the clear images of a variety of split phase chromosomes, thus overcoming the technical problem that because of a lot of Camellia magniflora chromosomes, a clear dissociation phase can be difficultly acquirable, and laying a good foundationfor systematic research of the configuration change rule of Camellia magniflora chromosomes, variety improvement and crossbreeding.

Description

technical field [0001] The invention belongs to the field of plant cytology research, and in particular relates to a method for preparing chromosomes from silk of Camellia grandiflora. Background technique [0002] Big flower red camellia ( Camellia Magniflora ) is a rare natural polyploid species in the genus Camellia of the family Theaceae. Its flowers are as big as peonies and bright red; oil extracted from tea seeds is not only high-grade edible oil, but also can be made into high-grade cosmetics. It has high ornamental value and Economic Value. It is distributed in low mountain areas and has strong cold resistance and disease resistance, so it is an important genetic germplasm resource. The red camellia group (Sect. Camellia) has a large amount of flowers, but the flower diameter is small and the resistance is poor. Therefore, it is one of the most potential ways to cultivate large-flowered and highly resistant ornamental varieties to carry out the interspecific hybr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/31
Inventor 贾文庆陈韵何松林李纪元郭英姿刘会超穆金艳王艳丽朱小佩杜晓华陈晓叶
Owner HENAN INST OF SCI & TECH
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