A lip gloss and brush assembly includes a container, a fixed member secured in the container, a rotation member rotatably mounted in the fixed member, a brush mounted in the rotation member, and a cover mounted on the container and secured to the rotation member. Thus, the rotation member is rotated by the cover to open or close flow of the lip gloss contained in the inside of the container, so that the lip gloss and brush assembly is operated easily and conveniently. In addition, the flow of the lip gloss is controlled by rotation of the cover, so that the lip gloss is allowed to flow smoothly and constantly without being choked, thereby enhancing the working condition of the lip gloss and brush assembly.