High-efficiency cross breeding method of mung bean

Abstract

The invention belongs to the technical field of hybridization methods or devices for mung beans, and discloses a high-efficiency cross breeding method of mung beans. The high-efficiency cross breedingmethod comprises the following steps of, three days before hybridization work, spraying brassinolide with a concentration of 50 milligrams per liter and boric acid with a concentration of 1,000 milligrams per liter; performing the emasculation of a female parent: in the afternoon of the day before pollination, selecting an appropriate flower bud of the female parent, separating a vexillum and anala by using the tips of tweezers, longitudinally splitting a carina, removing 10 stamens from the base of a filament, completely removing other large and medium flower buds and already borne pods, which belong to the same flower cluster with an emasculated flower, and hanging a board on a pedicel; performing the pollination: sucking 200 microliters of mung-bean pollen suspension mixed liquor by using a pipette, and injecting 10 to 20 microliters of the mung-bean pollen suspension mixed liquor on a stigma of the female parent each time; and clearly writing a male parent, the female parent andhybridization time on the board hung on the pedicel. By using the high-efficiency cross breeding method, the work efficiency is increased by 225%, and the hybridization success rate is increased by 17.4%.

Description

technical field [0001] The invention belongs to the technical field of hybridization methods or equipment, and in particular relates to a high-efficiency mung bean hybrid breeding method. Background technique [0002] At present, the commonly used prior art in the industry is as follows: mung bean is a cleistogamous pollinated crop, and its flower stalk is short and thin, and it is easy to fall off when subjected to external force; its style is curved and slender, easy to break when artificial pollination, and hybridization is difficult. About mung bean hybridization method, many scholars at home and abroad have been constantly exploring. At present, the mung bean hybridization method announced by Liu Yuping and Tian Jing in 1997 is commonly used in China. The hybridization success rate of this method is about 5 times higher than that of the method announced before 1991. However, this method requires a male flower to pollinate a female flower. The number of male parent flow...

AI Technical Summary

Problems solved by technology

[0004] (1) The traditional method of "one male flower pollinating one female flower" has a large number of male flowers and takes a long time to take flowers; the method of pollination with keel petals is difficult to operate, resulting in the best pollination of mung beans time, the hybridization rate is slower

[0005] (2) the mung bean hybridization success rate of prior art is lower, and the operation of cover keel flap easily causes harm to female parent style and stigma easily, causes 2 damages to female parent style, reduces hybridization success rate

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