A kind of three-dimensional staining method of korla fragrant pear filament cells

Abstract

The invention discloses a three-dimensional staining method for Korla fragrant pear filament cells. During the flowering stage of Korla fragrant pear, the Korla fragrant pear filaments are picked and fixed in FAA liquid, the FAA liquid is replaced three times, and the fixative solution containing the filaments is stored at 4 ± In a 0.5°C refrigerator, sample sections were prepared by soaking, embedding, and paraffin block techniques, and the sections were stained with the improved hematoxylin staining solution to make the sections appear black, and the sections were washed and used in distilled water. The aluminum chloride solution separates the color of the slices to make permanent slices, bakes the permanent slices in an oven at a temperature of 38°C-42°C for 12-24h, until the permanent slices are dried, takes pictures with a high-power microscope, and observes the Korla pear flower silk cell structure. The method of the invention can clearly color the epidermis cells, filament middle layer cells and conduction tissue cells of Korla fragrant pear flower silk cells, clearly distinguish them, and has a three-dimensional staining effect.

Description

technical field [0001] The invention relates to the technical field of biological cells, in particular to a technical field related to three-dimensional staining of Korla fragrant pear flower silk cells. Background technique [0002] Korla fragrant pear is a kind of Xinjiang pear belonging to the subfamily of Pyridaceae of Rosaceae. It is the main fruit tree variety in Bazhou and Aksu regions of Xinjiang. Famous all over the world, it has become one of Xinjiang's important foreign exchange-earning fruits. According to the 2011 data from the Xinjiang Forest and Fruit Office, the cultivated area of ​​fragrant pears in Korla, Xinjiang has reached 51,000 hm 2 , the fruit-bearing area is about 36,400 hm 2 , the annual output is about 700,000 tons, the sales are mainly in the domestic market, and the output value is about 2 billion yuan. Korla Fragrant Pear is known as the "Prince of Fruits". It has multiple functions such as protecting the heart, reducing fatigue, enhancing my...

AI Technical Summary

Problems solved by technology

[0006] In view of the fact that there is no easy-to-operate three-dimensional staining method for Korla fragrant pear filament cells, the epidermal cells, middle layer cells and conducting tissue structures of Korla fragrant pear filaments are clearly stained, and the research technology status of each part of the cells is clearly distinguished, and the existing common The preparation of the solution by the whole-body staining method of Ai’s hematoxylin is relatively complicated, and it generally needs to be cooked for more than 2 months after being exposed. Shortcomings and deficiencies, the present invention aims to provide a method for three-dimensional dyeing of Korla fragrant pear floral filament cells, which can clearly color and clearly distinguish the epidermal cells, filament middle layer cells and conducting tissue cells of Korla fragrant pear floral filament cells, and has a three-dimensional staining effect. And the operation method is simple, the staining time is easy to control, it is suitable for large-scale mechanized processing, and saves time and materials at the same time. It can be directly observed without covering the glass slide, and can also be made into permanent sections after treatment with alcohol solution and xylene. , used to study the internal structure of Korla fragrant pear filaments and the role of filaments in the process of pollen development

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