Method for obtaining pure line through inducing parthenogenesis of Zea mays by chemical agent

Abstract

The invention provides a method for obtaining a pure line through inducing parthenogenesis of Zea mays by a chemical agent. The method comprises the following steps: (1) preparing the chemical agent; (2) carrying out induction treatment: completely withdrawing tassels of plants required to be treated when the tassels appear before stamen stalks are drawn, strictly bagging female ear flowers, after the stamen stalks are drawn for 1 to 3 days, and scissoring each stamen stalk drawn portion into a notch by a pair of disinfected scissors in early morning of the same day of treatment before dew is evaporated; putting an induction agent dipped absorbent cotton ball at each notch, not taking off absorbent cotton balls so as to enable the absorbent cotton balls to be in full contact with the stamen stalks, and finally bagging paper bags again; and in the morning of the next day, injecting the induction agent to the stamen stalks without uncovering pollination bags, so as to carry out secondary induction; (3) carrying out harvesting; and (4) carrying out progeny testing. According to the method provided by the invention, the contamination caused by foreign pollen during the induction of the chemical agent is avoided, the setting percentage of induction and the number of grains of each ear are increased, and the aims of rapidly breeding the pure line and carrying out large-scale application are achieved.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, and in particular relates to a method for inducing corn parthenogenesis to obtain a pure line with chemical agents. Background technique [0002] Maize (Zea mays L.) is one of the earliest crops to apply heterosis, and inbred lines with strong adaptability are the prerequisite for this work. At present, the main methods of inbred breeding are: conventional breeding method and haploid breeding method. Conventional breeding methods generally require 6-8 years of continuous artificial bagging, self-crossing and selection, which have disadvantages such as long cycle, low selection efficiency, labor and financial resources. Haploid breeding is to use natural occurrence or artificial induction to produce haploid plants, obtain homozygous diploid plants through artificial or natural doubling, and then select inbred lines from them. The main ways of haploid production are: through the in vitro cu...

AI Technical Summary

Problems solved by technology

The disadvantages are: 1) The flowering period of the induced line and the basic material needs to be artificially pollinated and hybridized, and there is a problem that the flowering period of the two does not meet; 2) The basic material affects the color development of the induced contemporary grains, so it is difficult to select the induced contemporary grains of some materials; 3) Inducing contemporary grains requires manual identification of each seed to find haploid grains containing markers, which is a heavy workload and difficult to achieve large-scale application; 4) field management of haploid grains requires considerable care , and requires the cumbersome process of chromosome doubling

Disadvantages: 1) Anthers are cultured in vitro to obtain haploids, and the callus induction rate is greatly affected by the genotype of the culture material, and there are few inducible materials; 2) The frequency of pollen callus induction is low , pollen plant regeneration rate is low; 3) time-consuming and low efficiency

However, the current method has disadvantages: 1) foreign pollen pollution during chemical induction; 2) low seed setting rate and low seed setting per panicle

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