A method for evaluating the resistance of strawberry stamens to botrytis cinerea

A technology for Botrytis cinerea and stamens, applied in the field of agricultural plant germplasm resources evaluation, can solve problems such as no public reports

Active Publication Date: 2021-04-27
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Invented a kind of strawberry stamen to the evaluation method of Botrytis cinerea resistance accordingly, through literature search, do not see the public report of a kind of strawberry stamen identical with the present invention to the evaluation method of gray mold resistance

Method used

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  • A method for evaluating the resistance of strawberry stamens to botrytis cinerea
  • A method for evaluating the resistance of strawberry stamens to botrytis cinerea
  • A method for evaluating the resistance of strawberry stamens to botrytis cinerea

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Embodiment 1

[0032] A method for evaluating strawberry stamen gray mold resistance, the steps are:

[0033] 1. When strawberry flowers just start to bud, select 10 or 12 or 14 or 16 flowers that have just started to bud for each variety, and pick the flowers and flower stalks from the plant together;

[0034] 2. Insert the flowers into the solidified water agar, and culture them in vitro under constant temperature and light at 25°C for 24 hours, then remove the flowers whose petals have not fully unfolded, and ensure that there are more than 9 flowers with fully unfolded petals for each variety ;

[0035] 3. Use tweezers to remove all the petals from the flower;

[0036] 4, stick the botrytis cinerea conidia suspension with a writing brush, apply the conidia to the anthers of the stamens (the stamens include filaments and anthers, and the anthers here are a part of the stamens) to inoculate; Suspension preparation method: Botrytis cinerea mycelium blocks are inoculated on PDA medium, and...

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Abstract

The invention discloses a method for evaluating the resistance of strawberry stamens to botrytis cinerea. The steps are: A. picking off the flower and the flower stalk from the plant when the strawberry flower just begins to bud; B. inserting the flower in the solidified water In agar, in vitro culture under light conditions; C, use tweezers to pick off the petals from the flower; D, stick the conidia suspension of Botrytis cinerea with a brush, and apply the conidia to the anthers of the stamens for inoculation; Inoculate the botrytis cinerea mycelium block on the PDA medium; E, reinsert the flower in solidified water agar, and place it under constant temperature and light conditions for in vitro culture; F, remove the young strawberry fruit from the sepals; G 1. Measure the lesion length on the filament, grade the disease index of each stamen respectively; H, calculate the disease index of each variety respectively; 1, the disease index of each variety classify its botrytis cinerea resistance. The method is easy to operate and the operation is simple and convenient. This method can quickly identify the resistance to Botrytis cinerea in the sepals of strawberry cultivars.

Description

technical field [0001] The invention belongs to the technical field of evaluation of agricultural plant germplasm resources, and more specifically relates to a method for evaluating resistance to botrytis cinerea of ​​strawberry stamens. Background technique [0002] Botrytis cinerea is a kind of important plant pathogenic bacteria widely distributed in the world, which can harm more than 200 host plants such as fruits, vegetables, field crops, and ornamental plants, and cause botrytis cinerea. The above-ground parts of strawberry can be infected with gray mold, especially the flowers and fruits are most seriously damaged by gray mold. Strawberries are very sensitive to Botrytis cinerea within 2 to 3 days after flowering, and the field resistance to gray mold of different strawberry varieties is significantly different. Mildew incidence is very high. The existing resistance evaluation methods can only evaluate the resistance to Botrytis cinerea by observing the field sever...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18C12R1/645
CPCC12Q1/18G01N2333/37
Inventor 韩永超张庆华曾祥国过聪向发云
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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