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A kind of method utilizing ems mutagenesis to produce corn mutant

A mutant, corn technology, applied in the fields of botanical equipment and methods, application, plant gene improvement, etc., can solve the problems of poor generality of EMS mutagenesis method, and achieve the effect of good versatility and high mutation rate

Active Publication Date: 2020-01-24
CORN RES INST SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above problems, the present invention provides a method for producing corn mutants by EMS mutagenesis, which solves the problem that the single EMS mutagenesis method in the prior art has poor versatility in different corn inbred lines

Method used

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  • A kind of method utilizing ems mutagenesis to produce corn mutant
  • A kind of method utilizing ems mutagenesis to produce corn mutant
  • A kind of method utilizing ems mutagenesis to produce corn mutant

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A method utilizing EMS mutagenesis to produce corn mutants, comprising the following steps:

[0036] S1, pollen collection

[0037] Bagging the tassels of corn that is about to loose powder to collect corn pollen;

[0038] Select corn ears with good growth during the growth period for bagging, avoid ear pollination, and obtain corn plants to be pollinated;

[0039] S2, pollen mutagenesis treatment

[0040] Prepare the EMS-paraffin oil solution, remove the anthers from the pollen collected in S1 with a sieve, then add it to the EMS-paraffin oil solution, add 1g of the pollen from which the anthers have been removed for every 5ml of the EMS-paraffin oil solution, and perform mutagenesis according to the following steps : (1) irradiate at 30cm directly below the 15W UV lamp for 60s, (2) avoid light and stir at 500r / min for 30min, (3) irradiate at 30cm directly below the 15W UV lamp for 15s, (4) avoid light and stir at 500r / min for 15min , to obtain the mutagenic pollen ...

Embodiment 2

[0045] A method utilizing EMS mutagenesis to produce corn mutants, comprising the following steps:

[0046] S1, pollen collection

[0047] Bag the tassels of corn within 2 days of just beginning to loose powder, and collect corn pollen;

[0048] Select corn ears with good growth during the growth period and bag them to avoid ear pollination, and obtain corn plants to be pollinated;

[0049] S2, pollen mutagenesis treatment

[0050] Prepare the EMS-paraffin oil solution, remove the anthers from the pollen collected in S1 with a sieve, and then add it to the EMS-paraffin oil solution, add 1g of the pollen from which the anthers have been removed for every 10ml of the EMS-paraffin oil solution, and perform mutagenesis according to the following steps : (1) irradiate at 30cm directly below the 15W UV lamp for 30s, (2) avoid light and stir at 1000r / min for 20min, (3) irradiate at 30cm directly below the 15W UV lamp for 20s, (4) avoid light and stir at 1000r / min for 20min , to ob...

Embodiment 3

[0055] A method utilizing EMS mutagenesis to produce corn mutants, comprising the following steps:

[0056] S1, pollen collection

[0057] Bagging the tassels of corn that is about to loose powder to collect corn pollen;

[0058] Select corn ears with good growth during the growth period for bagging, avoid ear pollination, and obtain corn plants to be pollinated;

[0059] S2, pollen mutagenesis treatment

[0060] Prepare the EMS-paraffin oil solution, remove the anthers from the pollen collected in S1 with a sieve, then add it to the EMS-paraffin oil solution, add 1g of the pollen from which the anthers have been removed for every 8ml of the EMS-paraffin oil solution, and perform mutagenesis according to the following steps : (1) irradiate at 30cm directly below the 15W UV lamp for 45s, (2) avoid light and stir at 600r / min for 30min, (3) irradiate at 30cm directly below the 15W UV lamp for 20s, (4) avoid light and stir at 600r / min for 16min , to obtain the mutagenic pollen ...

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Abstract

The invention belongs to the technical field of corn breeding, and in particular relates to a method for producing corn mutants through EMS mutagenesis. Including pollen collection, pollen mutagenesis treatment and artificial pollination steps, wherein the collected pollen is added to the EMS-paraffin oil solution after the anthers are removed, and the mutagenesis is carried out according to the following steps: (1) irradiate under the ultraviolet lamp for 30-60s, (2) Stir in the dark for 20-30 minutes, (3) irradiate under the ultraviolet lamp for 15-30 seconds, (4) stir in the dark for 15-20 minutes; before artificial pollination, apply EMS‑on the ear filaments of the corn plants to be pollinated paraffin oil solution to obtain mutagenized filaments, and then artificial pollination. The invention utilizes the different mutagenesis mechanisms of ultraviolet radiation and EMS, combines the two mutagenesis methods, and has good versatility, and also performs mutagenesis treatment on filaments to cause mutations in some genes in the pistil, with a high mutation rate.

Description

technical field [0001] The invention belongs to the technical field of corn breeding, and in particular relates to a method for producing corn mutants through EMS mutagenesis. Background technique [0002] Corn is the second largest crop in the world after wheat and rice. The development space of new varieties bred by hybridization is small, and the variety's stress resistance is not comprehensive. Using mutagenesis technology to breed new varieties of corn has a wide range of variation and can produce new gene mutants that are difficult to appear in natural mutations. Currently commonly used mutagenesis methods include chemical mutagenesis, electric radiation mutagenesis, biological mutagenesis, and space mutagenesis. Ethyl methanesulfonate is a common and most widely used mutagen in maize. Using EMS (ethyl methylsulfonate) to treat maize pollen mutagenesis technology can not only create new mutant germplasm resources with economic value, but also provide maize with high ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/06
CPCA01H1/02A01H1/06
Inventor 赵变平王建军邵林生杨俊伟陈朝辉李彦良贾鑫罗绮王志虹焦建伟
Owner CORN RES INST SHANXI ACAD OF AGRI SCI
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