Method for producing cytoplasmic male sterility of fresh corn silk
A technology of male sterility and production methods, applied in botany equipment and methods, horticulture, applications, etc., can solve the problems of waste of beneficial plant resources, backward production and processing methods, and less luster, so as to reduce production costs and labor input , the effect of increasing yield and hybrid seed yield
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Embodiment 1
[0038] In this example, an excellent corn variety "Yuyu 22" was selected to produce fresh corn silks in large quantities in Yangling, Shaanxi.
[0039] Specifically include the following steps:
[0040] The first step is to select an excellent corn variety "Yuyu 22" (National Approved Jade: 20000012) (variety right number: CNA19990006.X) suitable for the production of corn silk. This variety has the characteristics of thick filaments, long filaments, high yield and good quality.
[0041]"Yuyu 22" is a large-ear corn hybrid made by the Maize Research Institute of Henan Agricultural University using the introduced line "Zong 3" and the self-selected line "Yu 87-1". It has high yield, high quality, disease resistance, Wide fit, large spike and other characteristics [Liu Zonghua, Chen Weicheng, Luo Fuhe et al. Breeding research on large spike-type corn hybrid Yudan 8703 J. Journal of Henan Agricultural University, 1997, 31 (addition): 1-4]. At the same time, they bred the female...
Embodiment 2
[0048] Embodiment 2: The present embodiment produces fresh corn whiskers in Yangling, Shaanxi in large quantities with the corn hybrid "Shendan No. 10", and it specifically includes the following steps:
[0049] The first step is to select an excellent corn variety "Shendan No. 10" (National Examination Jade: 990003) (variety right number: CNA20010072.6) suitable for the production of corn silk. This variety has the characteristics of thick filaments, long filaments, high yield and good quality.
[0050] In the second step, the conventional corn hybrid "Shendan 10" suitable for corn silk production was transformed into a sterile hybrid "CMS Shendan 10".
[0051] (A) Sterile gene transfer: the female parent Q1261 of the conventional corn hybrid "Shendan 10" was used as the male parent, and the C-type cytoplasmic sterile material was used as the female parent to perform hybridization to obtain the first generation of hybrid F1 sterile seeds;
[0052] (B) Backcross: plant the F1...
Embodiment 3
[0061] Example 3: In this example, the sterile corn hybrid "CMS Shendan No. 10" (National Examination: 990003) (variety right number: CNA20010072.6) and common corn hybrid Nongda 108 (National Examination: 2001002) were used A large amount of fresh corn silk is produced in Yangling, Shaanxi, which specifically includes the following steps:
[0062] The first step is to select an excellent corn variety "CMS Shendan 10" suitable for corn silk production. This variety has the characteristics of thick filaments, long filaments, high yield and good quality.
[0063] In the second step, the conventional corn hybrid "Shen Dan No. 10" suitable for corn silk production was transformed into a sterile hybrid "CMS Shen Dan No. 10" (same as Example 2).
[0064] The third step is the selection of the isolation area. The isolation adopts two methods of time and space isolation. Time segregation requires that the difference between the early sowing time of other corn fields and the corn sil...
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