Application of wdr1 gene in preparation of non-small cell lung cancer treatment and detection products

A non-small cell lung cancer and gene technology, applied in the field of molecular biology, can solve the problems that have not been reported in the research, achieve the effect of improving the current treatment status and good application prospects

Active Publication Date: 2021-04-27
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As a cancer with high metastatic potential, non-small cell lung cancer has not been reported on the relationship between WDR1 and non-small cell lung cancer

Method used

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  • Application of wdr1 gene in preparation of non-small cell lung cancer treatment and detection products
  • Application of wdr1 gene in preparation of non-small cell lung cancer treatment and detection products
  • Application of wdr1 gene in preparation of non-small cell lung cancer treatment and detection products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 WDR1 expression difference in NSCLC cancer tissue and its normal tissue

[0029] In this example, the total RNA of NSCLC cancer tissue and its normal tissue was extracted, and the WDR1 fragment gene and the internal reference gene b-actin were respectively amplified by real-time fluorescent quantitative PCR, and the expression difference of WDR1 gene in NSCLC cancer tissue and normal tissue was analyzed. The specific method includes the following steps:

[0030] (1) Collection of NSCLC cancer tissue and its normal tissue samples: In this example, NSCLC cancer tissue and its normal tissue were used as the detection objects. The samples of 39 NSCLC patients were collected from Nanjing Chest Hospital of Southeast University; Approved by the Medical Ethics Committee of Nanjing Chest Hospital, all cases obtained the informed consent of the patients;

[0031] (2) Extract total RNA from the NSCLC cancer tissue and its normal tissue to be tested: add Trizol (Invitrog...

Embodiment 2

[0037] Example 2 Evaluating the relationship between the expression level of WDR1 gene and the prognosis of NSCLC patients

[0038] In order to evaluate the relationship between the expression level of WDR1 and the prognosis of patients with non-small cell lung cancer, we performed Kaplan-Meier survival analysis through an online tool (http: / / kmplot.com) on a cohort of about 960 patients with non-small cell lung cancer Analysis shows (such as figure 2 shown), high WDR1 gene expression levels were associated with decreased overall survival (HR=1.43, log-rankP=3.7E-08). In addition, we also used another systematic software to evaluate the relationship between WDR1 gene expression level and patient prognosis (http: / / www.oncolnc.org), and we also obtained similar results (results such as image 3 shown): High levels of WDR1 gene expression were associated with reduced survival in lung adenocarcinoma (P=0.0428) and lung squamous cell carcinoma (P=0.193). The above results indica...

Embodiment 3

[0039] Example 3 Knocking down WDR1 at the cellular level to study the role of WDR1 in non-small cell lung cancer

[0040] We first adopted the method reported by Moffat J., et al., 2006, using the pLKO lentiviral expression system to construct stable knockdown WDR1 cells (shWDR1) and control cells in two non-small cell lung cancer cell lines A549 and H1299 (shCTL); the shRNA sequence we designed and used was as follows: shWDR1-GCTGGGAAGATCAAAGACATT (as shown in SEQ ID NO: 6); shCTL: GCAAGCTGACCCTGAAGTTCAT. The A549 cell lines in the WDR1 stable knockdown group and the control group were screened by lentivirus packaging, infection and antibiotic puromycin, and finally the cell selection was verified by Western blot. The Western blot experiment method is as follows: Cell protein was extracted by RIPA cell lysate, protein concentration was determined by BCA method, the same amount of protein samples were subjected to 10% SDS-PAGE electrophoresis, transferred to the membrane, blo...

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Abstract

The invention belongs to the technical field of molecular biology, and specifically relates to the application of WDR1 gene in the preparation of products for the treatment and detection of non-small cell lung cancer. The present invention discloses for the first time that the human WDR1 gene is a valuable biomarker in the process of tumor occurrence and development, and is expected to become a potential target for the treatment strategy of non-small cell lung cancer. The human WDR1 gene is used in the preparation of non-small cell lung cancer screening and detection products And non-small cell lung cancer treatment prognosis detection products have certain application value, provide certain clues and new perspectives for the early detection, diagnosis and prognosis of NSCLC, help to develop targeted drugs for NSCLC treatment, and improve the treatment status of NSCLC , has potential and good application prospects in the fields of tumor detection, diagnosis and treatment; the invention provides new theoretical basis and direction for clarifying the potential molecular pathway and mechanism of NSCLC occurrence and development.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to the application of WDR1 gene in the preparation of products for the treatment and detection of non-small cell lung cancer. Background technique [0002] Lung cancer is the leading cause of cancer-related death, affects millions of lives, and has become a serious public health problem. Among them, non-small cell lung cancer (Non-Small Cell Lung Cancer, NSCLC) accounts for about 85% of newly diagnosed lung cancer each year, and its histological types mainly include lung adenocarcinoma (50%) and lung squamous cell carcinoma (30%). Despite advances in early diagnosis and clinical treatment, lacking early symptom features, NSCLC patients are usually diagnosed at an advanced stage with a poor prognosis, with a 5-year survival rate of only 16% (regardless of tumor stage and size). Therefore, finding and identifying genes related to NSCLC tumors will be beneficial t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/12A61K31/7088A61P35/00
CPCA61K31/7088A61P35/00C12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/166
Inventor 袁白银张瑞瑞刘钟颖胡继盛王琼张同存
Owner WUHAN UNIV OF SCI & TECH
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