The present invention relates to a method for production of recombinant
casein, wherein the method comprises the steps of: (a) adding a microbial strain to a first
bioreactor, wherein said microbial strain has the capacity to express
casein, and said microbial strain comprises means for deactivation of
cell division; (b) culturing said microbial strain in the first
bioreactor, wherein the
cell division is active, thereby forming a continuously growing
biomass comprising cells of said microbial strain; (c) deactivating
cell division; (d) inducing expression of
casein; (e) after sufficient expression of casein, lysing said cells, thereby forming a
lysis broth, and separating said
lysis broth so that casein can be separated into a casein containing phase; (f) optionally treating the casein containing phase to induce formation of
casein micelles; (g) optionally further purifying and / or treating the
casein micelles, and (h) optionally producing caseinate. In other aspects, the invention also relates to a
bioreactor system, a recombinant casein, and products including a recombinant casein.