118results about "Protein composition from microorganisms" patented technology

A continuous method for preparing proteosome-amphiphilic determinant vaccines for parenteral or mucosal administration using diafiltration or ultrafiltration technology. The amphiphilic determinants include lipopolysaccharides from gram negative bacteria, e.g. S. flexneri, P. shigelloides and S. sonnei. Proteosomes are obtained from group B type 2b meningococci. The active proteosome-amphiphilic determinant complexes (non-covalent complexes) of the vaccine are formed using diafiltration or ultrafiltration to remove the detergent under non-static conditions. The use of diafiltration or ultrafiltration decreases processing time and the opportunity for contamination and further permits the use of ambient temperature and efficient scale-up. In addition, the process permits the reliable and continuous monitoring of the dializate which enhances the efficiency of the entire process. The time of dialysis for the production of a lot of vaccine is reduced from 7-10 days to less than 72 hours and usually less than 48 or 24 hours. The use of the process optimizes the presence of each antigenic component in the preparation of multivalent vaccines.

The preparation of a proteinaceous substance suitable for use in a foodstuff is described which comprises fungal fells of the order Mucorales. The cells are grown in a fermentor vessel in a liquid which is mixed during fermentation, after which the RNA content of the fungal cells is reduced to below 4% the fungal cells processed into an edible substance. This substance is then mechanically texturized into edible textured product for inclusion into foodstuffs, for example in the form of chunks as a meat substitute.

Provided herein are methods and compositions related to plant based meat substitutes which have properties similar to meat.

Microorganisms and bioprocesses are provided that convert gaseous substrates, such as renewable H2 and waste CO2 producer gas, or syngas into high-protein biomass that may be used directly for human nutrition, or as a nutrient for plants, fungi, or other microorganisms, or as a source of soil carbon, nitrogen, and other mineral nutrients. Renewable H2 used in the processes described herein may be generated by electrolysis using solar or wind power. Producer gas used in the processes described herein may be derived from sources that include gasification of waste feedstock and / or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.

A process for preparing non-odour white silkworm pupa protein from the degreased silkworm pupa powder includes dissolving saponizing, separation, regulating isopotential point, separating, washing, deodouring and cross-linking. Its advantages are high extracting rate, and high quality of product.

The invention provides a method for extracting a tea protein product. The method specifically comprises the following steps of: 1) pretreating raw materials; 2) preparing tea protein through ultrasonic-assisted alkali extraction; 3) decolorizing a tea protein solution; 4) concentrating a tea protein extracting solution; 5) precipitating the tea protein; and 6) washing the tea protein, refining and drying. The method has the advantages that: tea residue or low-grade tea such as summer-autumn tea and the like is used as a raw material, and a low-frequency ultrasonic circulation technology is used for assisting in extracting the tea protein; by the method, the protein is high in extraction rate and has high purity; the method is low in cost, simple and practicable, and easy to popularize; and the obtained tea protein product has green tea color, and the quality of the product meets food hygienic standard. A novel way of value-added transformation and recycling of tea residue and summer-autumn low-grade tea is provided; and a novel botanical protein product is developed, and the comprehensive utilization and fine and deep processing of tea resources are realized.

Aquafeed, animal feed, and other food products, as well as nutritional and pharmaceutical compounds, chemicals and biomaterials are important commodities that can be produced at commercial scale by fermentation of microorganisms. The present invention provides a method for producing these valuable multi-carbon compounds from simple gas feedstocks, such as carbon dioxide, hydrogen and oxygen, by cultivating a consortium of microbial cells specially selected for this purpose in an aqueous culture medium. In addition to exploiting inexpensive feedstocks, such as waste industrial gas for this cultivation, the platform described herein also provides the advantage of removing carbon dioxide and other waste gases from industrial emissions, which would otherwise contribute to global climate change. Furthermore, the cultivation of a microbial consortium can provide highly nutritious components to a feed blend that might not be available from a monoculture.

An elongate product comprises a filamentous fungus wherein the product has a dimension (A) measured in a first direction and a dimension (B) measured in a second direction, wherein dimension (A) is in the range 2 mm to 15 mm and dimension (B) is in the range 10 mm to 50 mm. The product is frozen mechanically in a relatively short time to produce ice-crystal sizes within the product of less than 20.0 μm, s. The product has a very long shelf life and when defrosted is found to perform like fresh paste when mixed with other ingredients.

The invention provides a technology for extracting high-purity potato protein through a hot flocculence method. At the significant end of the technology, small-granule starch and a fine-fiber mixture in potato starch processing and separation juice are separated, the purity and quality of the potato protein obtained through flocculence and separation at the final phase are improved, and meanwhile the phenomenon that heat exchange equipment and a pipeline are blocked due to starch dextrinization in the follow-up process is avoided; the potato protein is degenerated and flocculated through the hot flocculence method to be changed from the starch separation juice hydrosol body state into flocculence body solid to facilitate solid and liquid separation, the quality of the extracted protein is ensured, and meanwhile the physical flocculence method and the chemical flocculence precipitation method are used in a combined mode in cooperation with flocculence of chemical auxiliaries, so that the protein extraction rate and the protein separation effect are greatly improved, and the potato protein purity is improved. In addition, resource utilization of the potato protein is performed, more than 50% pollutant loads are reduced for follow-up sewage treatment, and the dual effects of resource utilization and pollutant control are achieved.

The invention provides a process for extracting yam mucoproteins from yams and belongs to the field of extracting functional biological macromolecular substances from natural materials to produce functional healthcare food. The extracting process comprises the main steps of (1) material selection; (2) leaching gelatinization; (3) enzymolysis; (4) standing; (5) adjustment of PH value; (6) flocculation; (7) precipitation; and (8) drying. The yield of the yam mucoprotein products prepared by the process is 4 to 5 percent, and the content of yam mucoprotein is 60 to 67 percent. According to the process provided by the invention, the organic solvent extraction in the prior art is replaced by the flocculation and natural precipitation, so the product cost is greatly saved, the environmental pollution is lowered, and the process has great significance for actual production.

The invention relates to light-yellow odourless degreasing silkworm pupa protein power and a preparation method thereof. According to the technical scheme, silk reeling silkworm pupae of which the skin and the gland are removed are selected as the raw material, silkworm pupa pretreatment is conducted, and coarse silkworm pupa powder is obtained; the coarse silkworm pupa powder is evenly mixed in degreasing solvent, ultrasonic degreasing treatment is conducted, centrifugal separation is conducted, and degreasing fluid and degreasing coarse pupa protein are obtained; drying and smashing are conducted on the degreasing coarse pupa protein, and the degreasing coarse pupa protein power is obtained; the degreasing coarse pupa protein power is evenly mixed in a sodium hydroxide solution, a silkworm pupa protein extracting solution is obtained, ultrasonic extraction treatment is conducted, macroporous resin is added into the silkworm pupa protein extracting solution, adsorption treatment is conducted, solid-liquid separation is conducted, resin and protein fluid are obtained, the protein fluid pH is adjusted, protein precipitation is collected, and wet silkworm pupa protein is obtained; vacuum drying and smashing are conducted on the wet silkworm pupa protein, and the light-yellow odourless degreasing silkworm pupa protein power is obtained. The light-yellow odourless degreasing silkworm pupa protein power and the preparation method thereof have the advantages that the technology is simple, the degreasing efficiency is high, effects of deodorization and decolorization are good, the treatment is low in temperature and short in time, the cost is low, the green and environmental protection is achieved, and the light-yellow odourless degreasing silkworm pupa protein power and the preparation method thereof can be used for industrial production.

The invention provides a method for extracting yellow mealworm active proteins by using an alkali-enzyme method, comprising the following steps: drying fresh yellow mealworm larvae to obtain dried yellow mealworm larvae of which the moisture content is lower than 5%, and smashing the dried yellow mealworm larvae; carrying out secondary digestion by taking petroleum ether of which the relative density is 0.64-0.66 as an extraction solvent; carrying out digestion with a 0.08-0.10mol / L NaOH solution; regulating the pH value to 7.0-7.5, carrying out enzymatically hydrolyzed extraction at a temperature of 55-60 DEG C by complex enzyme which is composed of neutral protease and papain at the mass ratio of 1:1-1:1.5; destroying enzyme; and centrifuging and dialyzing to obtain yellow mealworm protein powder. The yellow mealworm protein powder has the advantages of light yellow color and luster, beauty, fragrant odor as well as fine and smooth taste; and the protein content of the yellow mealworm protein powder reaches 94.5% and the extraction efficiency of the yellow mealworm protein powder reaches 75%.

The invention relates to a method for extracting macadimia nut proteins. The method mainly comprises the following steps of (1) smashing macadimia nuts, and separating oil of the macadimia nuts through infrared treatment and hydraulic cold squeezing; (2) mixing the obtained macadimia nut meal with pure water, treating the mixture through a colloid mill and an alkaline hardening, quenching and homogenizing machine, and then performing dynamic ultrahigh pressure treatment; and (3) adjusting the pH of the fruit meal to an isoelectric point to realize separation, and performing low-temperature spray drying to obtain the macadimia nut proteins. The technology disclosed by the invention is continuous and compact; the product is high in yield and high in quality.

The invention discloses a method for preparing semen cannabis protein powder from degreased semen cannabis residue, relating to the technical field of development and application of semen cannabis. The method comprises the steps of with degreased semen cannabis residue as a raw material, carrying out hot water protein extraction, filtrating, concentrating filtrate, and drying under the assistance of a drying aid to obtain the semen cannabis protein powder. According to the method, hot water is used as an extractant to improve the extraction efficiency of semen cannabis protein; as impurities such as an organic solvent and a acid-base reagent are not added, the extracted semen cannabis protein is not required to be purified, and the remaining semen cannabis residue is not polluted and can be used for preparing byproducts such as feeds or fertilizers, and therefore, the comprehensive utilization value of the semen cannabis is improved.

The invention discloses a method for preparing edible mushroom egg white powder. The method mainly comprises a step (1) of drying fresh edible mushrooms and smashing the mushrooms into powder; a step (2) of using alkaline water to extract edible mushroom dry powder, and ensuring that the egg white is dissolved in the alkali water to the largest extent; a step (3) of adjusting pH of the solution to an isoelectric point to make the egg white precipitate, taking the precipitated egg white, adding a small amount of water in the egg white, adjusting the pH of the protein liquid to be neutral, and performing low-temperature spraying drying to obtain the egg white powder. The method is simple in process, practical and high in egg white yield, makes the quality good, and is easy to popularize. The edible mushrooms include compound edible mushrooms or single edible mushrooms such as coprinus comatus, agaricus bisporus and pleurotus eryngii.

A process for preparing the vegetative protein from the seed dregs of Jatropha curcas tree, which are generated by squeezing oil, includes such steps as diluting the seed dregs by water, adding modifier (the mixed solution of H2O2 and NH4OH), heating to 100-120 deg.C and detoxicating reaction. Said vegetative protein can be used as the additive of feed.

The invention discloses a method for extracting selenoprotein from selenium-rich rice bran. The method comprises the following steps of performing ultrasonic extraction on fresh selenium-rich rice bran powder and a prepared extraction solution in an ultrasonic washing machine; then performing decoloring and performing centrifugation for precipitation; adjusting isoelectric points with citric acid, and performing centrifugation for collecting precipitate; removing impurities with a dialysis bag; and finally, performing freeze drying so as to obtain the selenoprotein. According to the method disclosed by the invention, a manner of reducing extraction and performing acid precipitation improved, so that selenoprotein is obtained, and the protein extraction rate is 80% or above; and the extracted selenoprotein is few in impurities, the protein content is as high as 80-95%, and the selenium content of the selenoprotein is as high as 0.50-0.58mg / kg. The method is simple and convenient, conditions are mild, the production technology is simple, industrial popularization is easy and the method has high utilization value.

The invention discloses a production method of a non-denatured pure sericin stock solution. By utilizing the production method, the pure sericin stock solution is produced by evading sericin denatured conditions. The production method is simple, low in cost and suitable for production of nutritious foods, health care products and products in other fields.

The invention provides a method for producing cottonseed germ materials used in low-temperature pretreatment production of gossypol-removed cottonseed protein. The method comprises: cleaning and removing impurities in delinted cottonseeds; husking the cottonseeds after impurity removal; obtaining mixture of cottonseed kernels and cottonseed husks; repeatedly separating the mixture by use of a screening and / or winnowing method; obtaining the cottonseed kernels in which the content of the cottonseed husks is less than or equal to 18 percent; softening the cottonseed kernels at a temperature lower than or equal to 80 DEG C; rolling the cottonseed kernels into germ sheets; adjusting quality at a temperature lower than or equal to 80 DEG C; and obtaining germ materials. The germ materials produced by use of the method have the advantages of good shape, high strength, less breakable property, good permeability during extraction, short seepage time, low content of residual oil and gossypol in meal after oil extraction and gossypol removal, capability of continuous production and capability of maintaining the original nutritional value of protein.

The invention discloses a method for extracting active protein from rough old tea. The method comprises the steps of destroying the cell wall of tea cell through ultrasonic-assisted extraction, cellulose treatment and pectase treatment, so as to ensure that active material is dissolved out to a maximum limit, extricating acidic protein in the tea through alkaline process, separating out tea protein through an isoelectric precipitation method, and performing zymohydrolysis to the tea protein, so as to improve performances of the tea protein again. As ultrasonic technology and complex enzyme treatment destroy the cell wall of the tea cell, and the structure of active material in the cell is also affected to a certain degree, the yield of tea protein samples obtained through the method is high; and after zymohydrolysis treatment, the solubility and the antioxidant activity of protein are obviously improved, so that the method has the advantages of high resource utilization rate, energy conservation, enhanced activity and the like.

Microorganisms and bioprocesses are provided that convert gaseous substrates, such as renewable H2 and waste CO2 producer gas, or syngas into high-protein biomass that may be used directly for human nutrition, or as a nutrient for plants, fungi, or other microorganisms, or as a source of soil carbon, nitrogen, and other mineral nutrients. Renewable H2 used in the processes described herein may be generated by electrolysis using solar or wind power. Producer gas used in the processes described herein may be derived from sources that include gasification of waste feedstock and / or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.

The invention discloses a process for preparing fly larva albumen powder by enzymatic hydrolysis of fly larvae. The process comprises the following specific steps: adding water to fresh fly larvae, pulping, and adjusting the pH as 6.0-7.0; adding an enzyme composition, raising the temperature to 45-55 DEG C, performing heat-preservation enzymatic hydrolysis for 4-7h, and performing enzyme deactivation, filtration, filtrate collection, concentration and drying to obtain the fly larva albumen powder, wherein the enzyme composition is formed by compound protease, neutral protease and flavourzyme according to the weight ratio of 1:(1-2):(0.5-1), and the addition amount of the enzyme composition is 2-5 weight percent of the fresh fly larvae. Compared with the prior art, the process has the advantages that the enzyme composition formed by the compound protease, the neutral protease and the flavourzyme according to a specific ratio performs enzymatic hydrolysis on the pulp of the fresh fly larvae under specific conditions, all enzymes cooperate in the enzymatic hydrolysis process, the degree of hydrolysis is effectively improved, and meanwhile, the prepared fly larva albumen powder does not taste bitter, and only has slightly fishy smell.

The invention relates to a production method of a proteoglycan protein compound functional capsule product from domestic fungus / alga and belongs to the field of edible fungi and alga deep processing and related product development and application. Edible fungi polysaccharide powder and algal polysaccharide powder which are prepared by an ultrasonic-assisted extraction technology and edible fungi protein powder and alga protein powder which are prepared by a salting-in technology are compounded according to a certain ratio, and a conventional capsule preparation technology is adopted, so as to prepare the proteoglycan protein compound functional capsule product. By analyzing biological functional activities of polysaccharides and protein powders from two different sources, scientific proportioning is carried out to obtain the capsule product which has advantages of rich nutrient, comprehensive biological activity and high utilization rate. In comparison with like products, the nutritional compound capsule provided by the invention can be used to remarkably enhance immunity of the organism and delay aging process. The proteoglycan protein compound functional capsule product is a health food which has market value.

The invention discloses an extracting method for high-purity sweet potato protein. The method comprises the following steps: (1) washing and crushing a sweet potato, sieving the crushed sweet potato, adding pure water, stirring and soaking, and taking supernatant; (2) centrifuging the supernatant obtained in the step (1), and taking the supernatant; (3) filtering or performing suction filtering on the supernatant obtained by the step (2) through a food-grade activated carbon layer; (4) slowly adding the filtrate obtained in the step (3) into food-grade alcohol under the stirring condition, and standing to obtain flocculent mixed liquid; (5) centrifuging the flocculent mixed liquid which is obtained in the step (4); (6) collecting the solid obtained in the step (5), and performing vacuum drying or freeze drying to obtain finished sweet potato protein. Compared with the conventional process, the extracting method for the sweet potato protein disclosed by the invention has the advantages that the process is simple and stable, conditions are mild, the environment-friendly effect is achieved, and the purity of the obtained sweet potato protein is 92 to 97 percent, and the purity is higher compared with the purity of the sweet potato protein of 72.4 percent obtained by the conventional process method.

The invention relates to a preparation method of edible and medicinal fungi protein peptide-zinc chelate. The preparation method comprises the following steps: extracting proteins from edible and medicinal fungus by an alkali-soluble acid precipitation method or an ammonium sulfate precipitation method; performing restricted enzymatic hydrolysis on the edible and medicinal fungi proteins by alkaline proteases, neutral proteases or compound proteases, so that an enzymatic hydrolysate of the edible and medicinal fungi proteins is prepared; and chelating zinc in inorganic zinc acetate or zinc sulfate with the edible and medicinal fungi protein peptide, so that the edible and medicinal fungi protein peptide-zinc chelate is prepared. The alkali-soluble acid precipitation method or the ammonium sulfate precipitation method utilized in the method is capable of obviously improving extraction rates of the edible fungi proteins; and the time of the enzymatic hydrolysis is controlled so as to effectively control degradation degrees of the proteins. A preparation technology of the polypeptide-zinc chelate is simple. The zinc-peptide chelate prepared by the preparation metahod is unique in chelate system and transport mechanism, and is easy to absorb, safe, non-toxic and low in cost; the zinc-peptide chelate is capable of supplementing amino acids and zinc at the same time, so that the zinc-peptide chelate will become the first choice among zinc supplements. The preparation method of edible and medicinal fungi protein peptide-zinc chelate provides a new idea to edible fungi applications.

The invention discloses a full-soluble edible mushroom protein and a preparation method thereof. The full-soluble edible mushroom protein is prepared by the following steps: unmodified edible mushroom protein is expanded under an alkaline condition, and then acid is added to adjust the edible mushroom protein to be neutral. According to the preparation method, the primary structure of the protein is not changed, the nutritional value of the protein can be reserved to the maximum extent, the obtained edible mushroom protein is free of exogenous additive components, the acceptance degree of consumers is improved to the maximum extent, reasonable utilization of edible mushroom byproducts is achieved, and resource waste and environmental pollution are effectively avoided.

The invention relates to a brassica oleracea protein powder preparation method, which comprises: preparing a juice by adopting at least one organ selected from the stem, the leaf and the flower of brassica oleracea as raw material, carrying out heating flocculation on the juice, filtering to obtain solid protein and a filtrate, concentrating the filtrate through a membrane, mixing the concentrated filtrate and the solid protein, homogenizing, and carrying out spray drying to obtain the brassica oleracea protein powder. According to the present invention, the method has characteristics of simple process and strong operability; and the experiment results prove that the obtained brassica oleracea protein powder has effects of lowering of cholesterol in blood fat, and can be used in the fields of food, health products, medicine, and the like.

The invention discloses an extraction method of an MD (musca domestica) insect active protein intermediate, comprising the following steps: cleaning and drying MD larva; after drying the MD larva by blowing at 40 DEG C, smashing the dried product; fully degreasing; adding 1-1.5% of alkali, and adding water for hydrolysis at constant temperature; secondarily desalting; and freezing and drying leached and desalted precipitate. Because the above technical scheme is adopted, technological operation difficulty is greatly lowered and production cost is greatly lowered by changing complex dialysis desalting with high cost into the secondary desalting; in the process of secondary desalting, hydrolyzate is required to be precipitated and AgNO3 is not needed to be used for detection, which protects the health of workers; and in the working procedure of drying the original substance by blowing at 40 DEG C after cleaning and drying, considerable energy can be saved, equipment investment and manual work are reduced if compared with the alkali dilution method of the Institute of Zoology of Chinese Academy of Sciences that the original substance is boiled for 15 minutes and is frozen and dried, and thus production cost is greatly lowered.