Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Polyvinylpyrrolidone solution and preparation method thereof

A polyvinylpyrrolidone and solution technology, applied in the field of polyvinylpyrrolidone solution and its preparation, can solve the problems of poor sperm plasma membrane integrity, poor calcium ion protection, inability to effectively maintain sperm function, low fertilization rate, and the like, so as to reduce calcium ions shedding, maintaining integrity, improving fertilization rate

Inactive Publication Date: 2018-11-23
瑞柏生物(中国)股份有限公司
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, in human assisted reproductive technology, the solution used for the deceleration of sperm motility before single sperm injection (ICSI) and the sample anti-sticking process in embryo PGD / S biopsy is only HEPES or MOPS buffered polyvinylpyrrolidone salt solution , this polyvinylpyrrolidone salt solution cannot effectively maintain the function of sperm, especially the integrity of the sperm plasma membrane and the protection of calcium ions, resulting in a very low fertilization rate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] The present invention will be further described below in combination with specific implementation methods.

[0033] The polyvinylpyrrolidone solution of the present invention is that taurine, serine, threonine, glycine, proline, chelating agent, polyvinylpyrrolidone are respectively according to 0.20-0.25mmol / L, 0.05-0.15mmol / L, The concentrations of 0.20-0.40mmol / L, 0.10-0.50mmol / L, 0.10-0.50mM / L, 0.01-0.05mmol / L, and 70-100g / L are dissolved in a saline solution containing antibiotics and indicators.

[0034] The chelating agent is tetracarboxylic acid ethylenediaminetetraacetic acid; the antibiotic is gentamicin with a concentration of 5-12 μg / ml; the indicator is phenol red with a concentration of 5.0-10.0 μg / ml;

[0035]The saline solution is a compound culture solution using HEPES or MOPS as a buffer, based on sodium, potassium, magnesium, and calcium ions, and using glucose, sodium pyruvate, and sodium lactate as energy substances; it contains: sodium chloride 97....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a polyvinylpyrrolidone solution and a preparation method thereof, and belongs to the field of assisted reproductive technology (ART). The polyvinylpyrrolidone solution provided by the invention is prepared by adding taurine, serine, threonine, glycine, proline, chelating agent EDTA and polyvinylpyrrolidone into a salt solution. Not only the integrity of sperm plasma membranes can be effectively maintained, but also EDTA can chelate calcium ions, reduce the loss of calcium ions during self-motion removal of sperms, in order to improve the fertilization rate of sperm injection, and also provide a strong guarantee for the safety of assisted reproductive technology.

Description

technical field [0001] The invention relates to a polyvinylpyrrolidone solution and a preparation method thereof, belonging to the technical field of human assisted reproduction. Background technique [0002] At present, in human assisted reproductive technology, the solution used for the deceleration of sperm motility before single sperm injection (ICSI) and the sample anti-sticking process in embryo PGD / S biopsy is only HEPES or MOPS buffered polyvinylpyrrolidone salt solution , this polyvinylpyrrolidone salt solution cannot effectively maintain the function of the sperm, especially the integrity of the sperm plasma membrane and the protection of calcium ions are poor, resulting in a very low fertilization rate. [0003] Taurine, also known as β-aminoethanesulfonic acid, was first isolated from bezoar. The pure product is colorless or white oblique crystals and is odorless. Taurine has stable chemical properties and is insoluble in organic solvents such as ether. It is a ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 冯怀亮
Owner 瑞柏生物(中国)股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com