45 results about "ICSI - Intracytoplasmic sperm injection" patented technology

The present invention relates generally to microelectromechanical systems (MEMS) devices for the manipulation of cells or groups of cells, such as oocytes, embryos, and sperm. In particular, the present invention relates to Cell Labeling MEMS devices (2F), Microinjection MEMS devices, IntraCytoplasmic Sperm Injection (“ICSI”) MEMS devices, Zona Coring MEMS devices, Enucleation MEMS devices, Enucleation / Nuclear Transfer MEMS devices, and Cytoplasmic Transfer MEMS devices. The present invention also relates to kits containing the MEMS devices of the present invention.

A container or dish used for the micro manipulation, micro injection, biopsy and fertilization of oocyte and embryo culture. The dish allows the user to more readily perform procedures used to fertilize oocyte, such as intracytoplasmic sperm injection (ICSI), biopsy embryos and perform additional procedures in used in assisted reproductive techniques (ART), human reproduction and in vitro fertilization (IVF) techniques. The invention will allow ease in use, the reduction in the number of micro tools used in the procedure, as opposed to conventonal dishes and procedures, and will allow the user to more readily locate the oocytes and embryos to be handled and worked on. The inventon will add repetitiveness, consistency and may result in better results and outcome of the procedures. The invention will also give the user a more ergonomically correct dish for these types of procedures and related protocols.

A device for transferring cells present in fluid media is provided which is especially useful for in vitro fertilization techniques and intracytoplasmic sperm injection procedures.

The present invention relates to systems and methods for automated, computer-controlled, tracking and manipulation of motile specimen. The systems and methods of the present invention are suited for tracking and manipulating sperm, particularly for Intracytoplasmic Sperm Injection (ICSI) procedures. The present invention also relates also to devices for holding motile cells, such as sperm, and non-motile cells, such as oocytes. The device of the present invention comprises a sealed chamber having an air outlet, one well for the motile cells, and another well for non-motile cells.

The invention provides a purpose for preparing supplementary reproduction medicine from the following raw material medicine in percentage by weight: 18 to 30 parts of radix rehmanniae preparata, 9 to 12 parts of dogwood, 8 to 16 parts of Chinese yam, 9 to 12 parts of fructus lycii, 8 to 16 parts of semen cuscutae and 8 to 16 parts of antler gum. The invention also provides a method for promoting ovarian ovum in vitro maturation. The method comprises the following steps that a, an immature ovarian ovum is taken; and b, the immature ovarian ovum is placed in M199 culture liquid, a microdrop method is adopted for culture for 24 hours, and a mature ovarian ovum is obtained, wherein 2 percent of medicine is contained in the culture liquid. The medicine can promote the growth and development of the immature ovarian ovum in vitro and mainly promotes the ovarian ovum core maturation, and in addition, the cytoskeleton abnormality probability of the mature ovarian ovum is not increased. The medicine can also generate the influence on the ovum passing capability of sperms, and the reliable basis is provided for clinically preventing and treating the male sperm dysfunction, improving the IVF (in vitro fertilization) success ratio and reducing the use rate of ICSI (intracytoplasmic sperm injection).

An integrated automated system comprising a microfluidic cassette, and methods of use thereof, for intracytoplasmic sperm injection assisted fertilization. The microfluidic cassette and the integrated automated system provides a complete set-up of human gametes for assisted in vitro fertilization, including proper cell stage recognition, gamete propulsion via microfluidic currents, microinjection of a spermatozoon into an oocyte, and subsequent embryo culture and monitoring, thus allowing widespread distribution of in vitro insemination by favoring affordability.

The invention provides a purpose for preparing supplementary reproduction medicine from the following raw material medicine in percentage by weight: 18 to 30 parts of radix rehmanniae preparata, 9 to 12 parts of dogwood, 8 to 16 parts of Chinese yam, 9 to 12 parts of fructus lycii, 8 to 16 parts of semen cuscutae and 8 to 16 parts of antler gum. The invention also provides a method for promoting ovarian ovum in vitro maturation. The method comprises the following steps that a, an immature ovarian ovum is taken; and b, the immature ovarian ovum is placed in M199 culture liquid, a microdrop method is adopted for culture for 24 hours, and a mature ovarian ovum is obtained, wherein 2 percent of medicine is contained in the culture liquid. The medicine can promote the growth and development of the immature ovarian ovum in vitro and mainly promotes the ovarian ovum core maturation, and in addition, the cytoskeleton abnormality probability of the mature ovarian ovum is not increased. The medicine can also generate the influence on the ovum passing capability of sperms, and the reliable basis is provided for clinically preventing and treating the male sperm dysfunction, improving the IVF (in vitro fertilization) success ratio and reducing the use rate of ICSI (intracytoplasmic sperm injection).

The invention relates to a method for producing transgenic buffalo embryos by applying intracytoplasmic sperm injection (ICSI) mediation. The method comprises the following steps of: 1, incubating buffalo sperms and transgenic deoxyribonucleic acid (DNA) together; 2, performing intracytoplasmic microinjection on the buffalo sperms; and 3, cultivating and identifying the transgenic buffalo embryos. By the method of the invention, the transgenic buffalo embryos are obtained successfully. In 102 injection spawns of the transgenic buffalo embryos, 61.8 percent of the injection spawns are subjected to cleavage, 17 blastospheres are obtained in embryos expressed by blastomere serving as enhanced green fluorescent protein (EGFP), 11 blastospheres express the EGFP, and the transgenic positive rate of the blastospheres is up to 64.7 percent; and after one of the transgenic embryos is cultured in vitro for a long time, the expression of the EGFP can be still seen during 2 to 7 days, so the method makes the important progress for producing transgenic buffalos further.

The invention provides a sperm treating method. The method comprises the following step of: (1) hatching sperm suspension at the temperature of between 0 and 4DEG C for 0.5 to 3 hours to obtain intracytoplasmic sperm injection (ICSI) sperms, wherein the sperm suspension contains sperms and sodium hydroxide (NaOH); and according to the total volume of the sperm suspension, the concentration of the sodium hydroxide is 5 to 30nM.

Intracytoplasmic sperm injection utilizing sex-selected equine spermatozoa to obtain viable sex selected embryos transferable to a recipient female equine mammal to obtain sex selected foals.

The invention provides a method for detecting chromosome abnormality of embryos by the aid of blastocyst cultivation solution without zona pellucida. The method which is particularly an in-vitro non-therapeutic method for detecting the chromosome abnormality of the embryos by the aid of the blastocyst cultivation solution includes steps of (a), providing the cultivation solution from blastocyst cultivation systems; (b), carrying out gene detection on the cultivation solution so as to authenticate whether chromosomes of the embryos are abnormal or not. The zona pellucida of the embryos in the blastocyst cultivation systems is removed before the embryos are cultivated, the embryos are cultivated in the blastocyst systems for 3-6 days, and preferably, the cultivation solution is separated from the cultivation systems after 4 days. The method has the advantages that contamination and interference risks due to redundant sperms and parent-source granular cells in IVF (in-vitro fertilization) and ICSI (intracytoplasmic sperm injection) technical procedures can be eliminated, and whether the chromosomes of the embryos are abnormal or not can be accurately authenticated.

The invention relates to a method for improving the developmental capacity of sheep mature oocytes after vitrification refrigeration, belonging to the technical field of biology. The method comprises the steps of micro-injection fertilization of sheep mature oocytes and activation of fertilized eggs, wherein the activation of the fertilized eggs adopts a direct current pulse-chemical activation-direct current pulse mode, and experiments prove that the embryonic developmental capacity of the vitrified sheep mature oocytes subject to is greatly improved after the vitrified sheep mature oocytes is subject to intracytoplasmic sperm injection and is activated by the activation method of the invention. Compared with the conventional external fertilization method, the cleavage rate and the blastocyst rate are respectively increased to 13.7% and 6.8%. At the same time, by comparing the chromosome ploidy of embryos subject to different treatments, the result indicates that the proportion of normal-ploidy embryos (71.5%) obtained by the ECE method is apparently higher than that (45.4%) obtained by the common chemical activation method and no obvious difference exists compared with the external fertilization groups.

The invention relates to a preferential sperm freezing culture device which comprises freezing slide glass, a sperm tank, a sperm swimming channel, a collecting tank and a blocking piece. The volume of the sperm tank ranges from 50 microliters to 500 microliters, the volume of the collecting tank ranges from 5 microliters to 20 microliters, the width of the sperm swimming channel ranges from 10 micrometers to 30 micrometers, and the sperm swimming channel is connected with the sperm tank and the collecting tank. The preferential sperm freezing culture device has the advantages that the preferential sperm freezing culture device is applicable to screening preservation of frozen sperm, motile sperm can be automatically separated by the design of the sperm tank, the sperm swimming channel andthe collecting tank, operation amount is decreased, mechanical damage to the sperm caused by manual picking is avoided, the proportion of usable motile sperm is increased, the freezing slide glass ofall existing sperm can be completely replaced, the preferential sperm freezing culture device can be applied to the fields of normal sperm optimization sperm periodic IVF (in-vitro fertilization), ICSI (intracytoplasmic sperm injection) or artificial insemination, sperm collection of oligospermia and asthenospermia patients, surgical sperm extraction of azoospermatism patients, sperm collection and the like, and has a wide popularization and application prospect.

A method for producing a monkey-derived embryonic stem cell comprising the steps of carrying out fertilization by insemination by in vitro fertilization or intracytoplasmic sperm injection using a monkey ovum and monkey sperms, thereby giving a fertilized ovum, allowing the fertilized ovum to differentiate into a blastocyst by in vitro culture, and establishing an ES cell line using the blastocyst; the monkey ES cell obtained by the method, a method for screening a reagent for specific differentiation into cell or tissue by using the ES cell; and a differentiated cell or differentiated tissue each differentiated from the ES cell. According to the present invention, applications of the embryonic stem cells to embryological studies clinical applications, experimental models, and the like on primates, studies of diseases, are expected.

The invention discloses a method for determining a position and a gesture of an ooecium in automatic intracytoplasmic sperm injection, which comprises the following steps of: converting a colored image into a grayscale image and performing median filtering processing; determining positions of a micro-adsorption needle and a micro injection needle, and determining the position and the gesture of the ooecium; performing binarization processing on the grayscale image by using an Otsu adaptive threshold method; averaging n pixels along each pixel of the outline of an ooecium region in sequence towards a central direction of the region to form a sequence S; solving a mean value Savg of the sequence S; judging whether the value of each point in S is within the range [Savg-Ts, Savg+Ts] or not; if so, detecting a circle with a radius of rp in the interior of the ooecium region by using a Hough circle detection method; mapping a coordinate from an image coordinate system to an ooecium local coordinate system to obtain the coordinate of (xp, yp); and calculating an ooecium pitch angle E1 and an ooecium azimuth angle Az through a spatial triangle relationship, and outputting a position coordinate (xc, yc) and a gesture coordinate (Az, E1) of the ooecium.

A container or dish is useful for the micro manipulation, micro injection, biopsy and fertilization in oocyte and embryo manipulation and culturing. The dish allows the user to more readily perform procedures used to fertilize oocytes by Intracytoplasmic sperm injection (ICSI) or other procedures, biopsy embryos and perform additional procedures for use in assisted reproductive techniques (ART), human reproduction and in-vitro fertilization (IVF). The invention allows ease in use, the reduction in the number of micro tools used in the procedure, as opposed to conventional dishes and procedures, and will allow the user to more readily locate the oocytes and embryos to be handled and worked on. The invention will add repetitiveness, consistency, improve efficacy and ease of procedure and may improve outcomes. The invention will also give the user a more ergonomically practical dish for these types of procedures and related protocols. Specimens other than oocytes and embryos can be treated in the container by means of micro manipulation.

A method for producing a monkey-derived embryonic stem cell comprising the steps of carrying out fertilization by insemination by in vitro fertilization or intracytoplasmic sperm injection using a monkey ovum and monkey sperms, thereby giving a fertilized ovum, allowing the fertilized ovum to differentiate into a blastocyst by in vitro culture, and establishing an ES cell line using the blastocyst; the monkey ES cell obtained by the method, a method for screening a reagent for specific differentiation into cell or tissue by using the ES cell; and a differentiated cell or differentiated tissue each differentiated from the ES cell. According to the present invention, applications of the embryonic stem cells to embryological studies clinical applications, experimental models, and the like on primates, studies of diseases, are expected.

The present invention provides a method that the genetic engineering technology is adopted to cultivate double-muscle meat sheep. The method is realized by that the propeptide sequence of a myostatin (myostatin) gene is cloned, and a non-specific express transgenic structure is constructed, after being purified, the myostatin (myostatin) gene and the non-specific express transgenic structure are transferred into a sheep embryo through an intracytoplasmic sperm injection technology, after a transgenic embryo is screened, and the transgenic embryo is transplanted into a surrogate ewe through the embryo transplantation technology, and finally the double-muscle meat sheep with the myostatin propeptide is obtained. The present invention disrupts the biological activity of the endogeneous myostatin with the method of the propeptide of the myostatin, to attain the purposes of improving the growth speed of the muscle of the sheep, increasing the output of the muscle, and cultivating the good meat sheep.

A container or dish used for the micro manipulation, micro injection, biopsy and fertilization of oocyte and embryo culture. The dish allows the user to more readily perform procedures used to fertilize oocyte, such as intracytoplasmic sperm injection (ICSI), biopsy embryos and perform additional procedures in used in assisted reproductive techniques (ART), human reproduction and in vitro fertilization (IVF) techniques. The invention will allow ease in use, the reduction in the number of micro tools used in the procedure, as opposed to conventional dishes and procedures, and will allow the user to more readily locate the oocytes and embryos to be handled and worked on. The invention will add repetitiveness, consistency and may result in better results and outcome of the procedures. The invention will also give the user a more ergonomically correct dish for these types of procedures and related protocols.

The invention relates to an intracytoplasmic sperm injection activation method, which belongs to the embryo engineering field. The invention uses ionomycin and DMAP to activate a fertilized ovum together, thereby the activation rate of oocytes and the cleavage rate are improved; after the ionomycin with a final concentration of 5 mu m and the DMAP with a final concentration of 2 mu m are used for activating the fertilized ovum, the cleavage rate is 60 to 65 percent.

A blade tip-provided micropipette holding apparatus has a tubular micropipette holder so constructed that a blade tip-provided micropipette to be inserted into a living cell can be mounted on a front-end portion thereof and a holder-holding device for holding the micropipette holder. The holder-holding device has a base member and a tubular rotating member which is rotatably held by the base member and into which the tubular micropipette holder can be penetrated. The tubular rotating member has holder position adjusting mechanisms each composed of a plurality of side holes formed on a side surface of the tubular rotating member at different positions of the side surface thereof in a circumferential direction thereof and holder position adjusting members each capable of entering into the tubular rotating member from the side holes and contacting an outer surface of the micropipette holder penetrating through the tubular rotating member.

Compositions and methods useful for processing animal semen for use in assisted reproduction. A composition, or colloid formulation, for separation of spermatozoa from a semen sample, the composition including at least one salt of an alkali metal and / or an alkaline earth metal, EDTA, a zwitterion buffer, silane-coated silica particles and water, the composition having a pH of 7.0-7.35 and an osmolarity of 300-345 mOsm. A method for preparing spermatozoa from a semen sample from a non-human animal by separating the spermatozoa from other semen constituents by centrifugation through a single layer of the composition, or colloid formulation. Also, a method for separating a sperm sub-population of interest from a semen sample from a non-human animal by providing a density gradient comprising at least two layers of the composition of the invention, each layer having a different density; separating the sperm sub-populations in the semen sample by centrifugation through the density gradient; and selecting the sperm sub-population of interest. Finally, spermatozoa prepared by these methods and the use of such spermatozoa in artificial insemination, in vitro fertilisation or intracytoplasmic sperm injection.

Methods, systems and apparatus for preparing sperm for ICSI by combining selective enrichment benefits of sperm gradient preparation, sperm swim-up preparation and hyaluronan-binding for selection of mature sperm into a single processing unit. The single processing unit of the invention reduces operator labor steps and time, is efficient, easy to use, offers quick processing time and provides highly motile, mature and specific individual sperm that retain desired sperm characteristics selected by each individual selection principal that are ready for capture and use in ICSI.

The invention provides a purpose for preparing supplementary reproduction medicine from the following raw material medicine in percentage by weight: 18 to 30 parts of radix rehmanniae preparata, 9 to 12 parts of dogwood, 8 to 16 parts of Chinese yam, 9 to 12 parts of fructus lycii, 8 to 16 parts of semen cuscutae and 8 to 16 parts of antler gum. The invention also provides a method for promoting ovarian ovum in vitro maturation. The method comprises the following steps that a, an immature ovarian ovum is taken; and b, the immature ovarian ovum is placed in M199 culture liquid, a microdrop method is adopted for culture for 24 hours, and a mature ovarian ovum is obtained, wherein 2 percent of medicine is contained in the culture liquid. The medicine can promote the growth and development of the immature ovarian ovum in vitro and mainly promotes the ovarian ovum core maturation, and in addition, the cytoskeleton abnormality probability of the mature ovarian ovum is not increased. The medicine can also generate the influence on the ovum passing capability of sperms, and the reliable basis is provided for clinically preventing and treating the male sperm dysfunction, improving the IVF (in vitro fertilization) success ratio and reducing the use rate of ICSI (intracytoplasmic sperm injection).

The present invention is providing a system based on artificial vision, and artificial intelligence that is capable of assisting the embryologist to select the best spermatozoa to be injected during an ICSI procedure, requiring the selection of a single sperm. It can identify the best spermatozoa from a sample, in real-time, based on their morphological and motility characteristics which are observed under the microscope at magnifications equal to or above 20×.Is an automatic analysis of sequences of images produced by a digital camera attached to a microscope in real-time. It uses computer vision algorithms to automatically detect and track each of the sperms present on every image of a video and compute a number of features related to the morphological characteristics and motility parameters. The features of each sperm are processed and then evaluated using a mathematical model which determines the quality of each sperm and ranks them accordingly.

The invention discloses a fertility preserving method of completely immovable sperms. The fertility preserving method includes following steps: 1, freezing the completely immovable sperms: collecting the completely sperms, taking a small part out, using laser to identify whether live sperms exist or not, if yes, freeze preserving, and if not, abandoning; 2, evaluating sperm survival state before and after freezing the completely immovable sperms: if the tails of sperms immediately wind up obviously after laser shooting, judging as live sperms, and judging as dead sperms if the tails of the sperms do not wind up and have no response to laser shooting. A circumstance that the completely immovable sperms can be freeze preserved is testified for the first time, and the sperms after being frozen have normal in-vitro fertilization capability. In addition, a method of efficiently freeze preserving fertility of these special groups is developed, and fertilization rate similar to that of fresh immovable sperms can be acquired through laser screening of live but completely immovable frozen sperms for intracytoplasmic sperm injection. Related reports do not appear at home and abroad.