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Preferential sperm freezing culture device

A technology for culturing equipment and sperm, applied in the field of medical devices, to avoid mechanical damage, good biocompatibility, and reduce sperm adhesion.

Pending Publication Date: 2018-12-25
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about a sperm culture device that has a simple structure, can separate motile sperm and immobile sperm, and can also be directly used for cryopreservation.
[0007] To sum up, there is an urgent need for a culture device with a simple structure, which can be used for cryopreservation of sperm, and can separate motile and immobile sperm by itself, and this kind of slide has not yet been published.

Method used

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  • Preferential sperm freezing culture device
  • Preferential sperm freezing culture device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Please refer to figure 1 , figure 1 It is a structural diagram of a frozen slide of a preferred sperm cryogenic culture equipment of the present invention, the frozen culture equipment includes a frozen slide 1, a semen pool 11, a sperm swimming channel 12, a collection pool 13, and a blocking sheet 14; the semen pool 11 has a volume of 50 to 500 microliters; the collection pool has a volume of 5 to 20 microliters; the gap formed by the blocking piece 14 and the inner surface of the slide is the sperm swimming channel 12 .

[0038] Please refer to figure 2 , figure 2 is a side perspective view of the slide. The narrow channel formed by the blocking sheet 14 and the frozen slide 1 is the sperm swimming channel 12; the sperm swimming channel 12 connects the semen pool 11 and the collection pool 13; the sperm swimming channel connects the semen pool 11 partly The connecting pool 13 is spacious; the sperm swimming channel 12 has a width of 10-30 microns.

[0039] It ...

Embodiment 2

[0050] This embodiment is basically the same as Embodiment 1, the difference is that the semen pool 11, collection pool 13 and sperm swimming channel 12 in this embodiment are all detachably connected to the frozen slide. The effect of this embodiment mode is: it can be directly frozen in liquid nitrogen, and the separated sperm can also be transferred to other frozen slides for freezing.

[0051] A preferred sperm freezing culture device of the present invention is easy to use, and can automatically separate motile sperm by using the design of the semen pool, sperm swimming channel, and collection pool, reducing the amount of operation, avoiding mechanical damage to sperm manually selected, and improving the usable motile sperm It can completely replace the frozen slides of all existing sperm. It can be applied in the following fields: IVF, ICSI or artificial insemination in the cycle of normal semen with preferred sperm, sperm collection for patients with oligoasthenozoosperm...

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Abstract

The invention relates to a preferential sperm freezing culture device which comprises freezing slide glass, a sperm tank, a sperm swimming channel, a collecting tank and a blocking piece. The volume of the sperm tank ranges from 50 microliters to 500 microliters, the volume of the collecting tank ranges from 5 microliters to 20 microliters, the width of the sperm swimming channel ranges from 10 micrometers to 30 micrometers, and the sperm swimming channel is connected with the sperm tank and the collecting tank. The preferential sperm freezing culture device has the advantages that the preferential sperm freezing culture device is applicable to screening preservation of frozen sperm, motile sperm can be automatically separated by the design of the sperm tank, the sperm swimming channel andthe collecting tank, operation amount is decreased, mechanical damage to the sperm caused by manual picking is avoided, the proportion of usable motile sperm is increased, the freezing slide glass ofall existing sperm can be completely replaced, the preferential sperm freezing culture device can be applied to the fields of normal sperm optimization sperm periodic IVF (in-vitro fertilization), ICSI (intracytoplasmic sperm injection) or artificial insemination, sperm collection of oligospermia and asthenospermia patients, surgical sperm extraction of azoospermatism patients, sperm collection and the like, and has a wide popularization and application prospect.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a preferred sperm freezing culture. Background technique [0002] In vitro fertilization-embryo transfer (IVF-ET), also known as "test-tube baby", refers to the process of taking eggs and sperm from the human body, fertilizing them outside the body, developing them into embryos, and then transplanting them back into the mother's uterus to achieve the purpose of conception. a technique. ICSI (Intracytoplasmic sperm injection) is intracytoplasmic sperm injection technology, which is the second generation of "test-tube baby". This technology uses a microscopic operating system to inject a single sperm into an egg to fertilize it. The implementation period is very long, or some patients do not have the conditions to raise children at this time, and the sperm needs to be frozen first, and reserved for artificial insemination or test-tube baby in the future. [0003] At prese...

Claims

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Application Information

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IPC IPC(8): C12M1/00A01N1/02
CPCA01N1/0268C12M47/04
Inventor 公跃华洪艳李铮姚晨成
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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