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Method for detecting chromosome abnormality of embryos by aid of blastocyst cultivation solution without zona pellucida

A culture medium and chromosome technology, applied in the fields of biomedicine and molecular cell biology, can solve the problems of blastocyst culture pollution, detection interference, etc.

Pending Publication Date: 2016-11-09
XUKANG MEDICAL SCI & TECH (SUZHOU) CO LTD
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Problems solved by technology

However, the adhered maternal granulosa cells on the zona pellucida will still contaminate the blastocyst culture and interfere with subsequent detection

Method used

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  • Method for detecting chromosome abnormality of embryos by aid of blastocyst cultivation solution without zona pellucida
  • Method for detecting chromosome abnormality of embryos by aid of blastocyst cultivation solution without zona pellucida
  • Method for detecting chromosome abnormality of embryos by aid of blastocyst cultivation solution without zona pellucida

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Embodiment 1

[0126] Utilize the detection method of the present invention (promptly stripping off the embryo zona pellucida at the morula stage, and then changing the medium for culture), two IVF embryos (sample C and D) are respectively detected by blastocyst cell biopsy and blastocyst culture medium The method of detection assesses its chromosomal status. The detection method of blastocyst culture fluid obtained the same result as the detection method of blastocyst cells.

[0127] The results of the next-generation sequencing data showed that in the C sample, the blastocyst culture fluid detection method ( Figure 3a ) and blastocyst cell detection method ( Figure 3b ) were found to be chromosomally normal. In sample D, the detection method of blastocyst culture medium ( Figure 4a ) and blastocyst cell detection method ( Figure 4b ) were found to have deletions in some segments of chromosome 2 and amplifications in some segments of chromosome 4.

[0128] Implanting the fertilized ...

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Abstract

The invention provides a method for detecting chromosome abnormality of embryos by the aid of blastocyst cultivation solution without zona pellucida. The method which is particularly an in-vitro non-therapeutic method for detecting the chromosome abnormality of the embryos by the aid of the blastocyst cultivation solution includes steps of (a), providing the cultivation solution from blastocyst cultivation systems; (b), carrying out gene detection on the cultivation solution so as to authenticate whether chromosomes of the embryos are abnormal or not. The zona pellucida of the embryos in the blastocyst cultivation systems is removed before the embryos are cultivated, the embryos are cultivated in the blastocyst systems for 3-6 days, and preferably, the cultivation solution is separated from the cultivation systems after 4 days. The method has the advantages that contamination and interference risks due to redundant sperms and parent-source granular cells in IVF (in-vitro fertilization) and ICSI (intracytoplasmic sperm injection) technical procedures can be eliminated, and whether the chromosomes of the embryos are abnormal or not can be accurately authenticated.

Description

technical field [0001] The invention relates to the fields of biomedicine and molecular cell biology, in particular to a method for detecting embryo chromosomal abnormalities by using blastocyst culture fluid without zona pellucida. Background technique [0002] Assisted reproductive technology has become an important means of treating infertility. With the continuous improvement of the technical level, the clinical pregnancy rate of assisted reproductive technology is also increasing, but the embryo implantation rate has not increased significantly. The quality of embryos is the main factor affecting embryo implantation. At present, the selection of high-quality embryos is mainly based on the morphological scoring of embryo development, which is too subjective to fundamentally distinguish the quality of embryos. Studies have shown that even some high-quality blastocysts with good morphology are aneuploid, or the morphological score of chromosomal euploid embryos is not high...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/68C12Q1/6888
Inventor 陆思嘉蔡立义任军马婷方锐
Owner XUKANG MEDICAL SCI & TECH (SUZHOU) CO LTD
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