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Method for culturing ''double-muscle'' mutton sheep by using gene engineering technique

A technology, gene technology, applied in the field of sheep breeding

Inactive Publication Date: 2008-03-19
TIANJIN INST OF ANIMAL HUSBANDRY & VETERINARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, this method has only been successful in experimental mice, and there are still no reports of success in sheep and other livestock breeds

Method used

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  • Method for culturing ''double-muscle'' mutton sheep by using gene engineering technique

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Experimental program
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Effect test

Embodiment Construction

[0015] The following is a description of the use of myostatin propeptide non-specific and muscle-specific expression structures to produce transgenic animals and breed new breeds of "double-muscle" sheep.

[0016] The specific method is (1) constructing a transgenic structure for non-specific expression of myostatin propeptide; (2) a method for cultivating transgenic sheep using the above-mentioned transgenic structure.

[0017] 1 Construction of transgenic construct for non-specific expression of myostatin propeptide

[0018] (1) Extraction of Sheep Total RNA

[0019] Take freshly slaughtered sheep muscle tissue, quickly put it into liquid nitrogen, take it to the laboratory, take 50-100 mg of tissue in a pre-cooled mortar, grind it into powder, and pay attention to adding liquid nitrogen to prevent RNA degradation. Put 100 mg of the ground powder into a centrifuge tube filled with 1 mL of cold Trizol, shake and mix, and place at room temperature for 5-6 minutes. Take 0.2mL...

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Abstract

The present invention provides a method that the genetic engineering technology is adopted to cultivate double-muscle meat sheep. The method is realized by that the propeptide sequence of a myostatin (myostatin) gene is cloned, and a non-specific express transgenic structure is constructed, after being purified, the myostatin (myostatin) gene and the non-specific express transgenic structure are transferred into a sheep embryo through an intracytoplasmic sperm injection technology, after a transgenic embryo is screened, and the transgenic embryo is transplanted into a surrogate ewe through the embryo transplantation technology, and finally the double-muscle meat sheep with the myostatin propeptide is obtained. The present invention disrupts the biological activity of the endogeneous myostatin with the method of the propeptide of the myostatin, to attain the purposes of improving the growth speed of the muscle of the sheep, increasing the output of the muscle, and cultivating the good meat sheep.

Description

technical field [0001] The invention relates to a new method of sheep breeding, in particular to a method for cultivating "double-muscle" mutton sheep with increased meat production rate, rapid growth, high feed conversion rate and good muscle quality by using transgenic technology. Background technique [0002] With the improvement of consumers' living standards, the demand for livestock and poultry meat products continues to increase. Because sheep mainly use natural grass as the main feed, the forage grass is less polluted and belongs to safe and green food, so it has been favored by consumers. But at present, the global mutton production is still low and it is difficult to meet the market demand. Therefore, as far as the world is concerned, the mutton market has a large space for development, and thus mutton sheep breeds with outstanding meat production performance are needed. [0003] In traditional livestock breeding methods, it is necessary to rely on animal phenoty...

Claims

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Application Information

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IPC IPC(8): C12N15/85C12N15/05C12Q1/68
Inventor 马毅丁伯良张国伟温叶飞李敏王超李向臣
Owner TIANJIN INST OF ANIMAL HUSBANDRY & VETERINARY
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