Fertility preserving method of completely immovable sperms

A preservation method and fertility technology, which is applied in the field of fertility preservation of completely immobile sperm, and can solve the problems of sperm motility and immobility

Inactive Publication Date: 2016-06-15
冯贵雪 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, some patients often obtain sperm with little vitality or even no movement at all.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This method of fertility preservation with complete immobility of spermatozoa consists of the following steps:

[0025] ① Completely immobile sperm freezing method:

[0026] After collecting completely immobile sperm, take a small part out, use laser identification, if there are surviving sperm, it can be judged as cryopreservation, if there is no surviving sperm, it will be discarded; the remaining semen is sucked to 100 μL (microliter), and put into a special micropipette Centrifuge directly with a low-speed centrifuge in the sperm freezing tube; remove the supernatant after centrifugation, and then add sperm freezing liquid with a volume of 1:1 with the sperm precipitation, mix well, and make the total volume of the final suspension be 10 μL; Place the cryovial with sperm suspension 2 cm away from the liquid nitrogen surface, fumigate for 3 minutes, and then put it directly into liquid nitrogen for cryopreservation; when thawing, directly put the cryovial into a preh...

Embodiment 2

[0033] This method of fertility preservation with complete immobility of spermatozoa consists of the following steps:

[0034] ① Completely immobile sperm freezing method:

[0035] After collecting completely immobile sperm, take a small part out, use laser identification, if there are surviving sperm, it can be judged as cryopreservation, if there is no surviving sperm, discard it; absorb the remaining semen to 200 μL (microliter), put it into a special micropipette Centrifuge directly with a low-speed centrifuge in the sperm freezing tube; remove the supernatant after centrifugation, and then add sperm freezing liquid with a volume of 1:1 with the sperm precipitation, mix well, and make the total volume of the final suspension be 10 μL; Place the cryovial with sperm suspension at a distance of 3 cm from the liquid nitrogen surface, fumigate for 5 minutes, and then put it directly into liquid nitrogen for cryopreservation; when thawing, put the cryovial directly into a prehea...

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PUM

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Abstract

The invention discloses a fertility preserving method of completely immovable sperms. The fertility preserving method includes following steps: 1, freezing the completely immovable sperms: collecting the completely sperms, taking a small part out, using laser to identify whether live sperms exist or not, if yes, freeze preserving, and if not, abandoning; 2, evaluating sperm survival state before and after freezing the completely immovable sperms: if the tails of sperms immediately wind up obviously after laser shooting, judging as live sperms, and judging as dead sperms if the tails of the sperms do not wind up and have no response to laser shooting. A circumstance that the completely immovable sperms can be freeze preserved is testified for the first time, and the sperms after being frozen have normal in-vitro fertilization capability. In addition, a method of efficiently freeze preserving fertility of these special groups is developed, and fertilization rate similar to that of fresh immovable sperms can be acquired through laser screening of live but completely immovable frozen sperms for intracytoplasmic sperm injection. Related reports do not appear at home and abroad.

Description

technical field [0001] The invention relates to a method for preserving the fertility of sperm, in particular to a method for preserving the fertility of completely motile sperm. Background technique [0002] At present, semen cryopreservation technology has a history of more than 200 years. The cryopreservation of human semen dates back to the late 1940s. Semen cryopreservation is mainly used in human sperm banks and preservation of fertility in my country. Researchers at home and abroad have established a relatively mature semen cryopreservation system through decades of screening of semen freezing procedures, freezing diluents, and thawing fluids. Reproductive centers across the country have a relatively large demand for cryoprotectants for human semen, but almost all of the cryopreservatives for human semen on the market are monopolized by foreign biological companies, and only some scientific research institutes in China configure cryoprotectants for livestock and pou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 冯贵雪陈焕华张波周红汪彩珠舒金辉林若芸甘贤优
Owner 冯贵雪
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