Primer, kit and method for detecting low grain protein content of wheat, and applications of method

Abstract

The invention discloses a primer, a kit and a method for detecting the low grain protein content of wheat, and applications of the method. An upstream primer sequence of the primer is shown as SEQ ID NO: I, and a downstream primer sequence of the primer is shown as SEQ ID NO: II. The method for detecting the low GPC (grain protein content) of wheat provided by the invention is reliable, simple and convenient, and practical, and has a significant application prospect in the wheat genetic resource assessment and the breeding molecular marker-assisted selection, and meanwhile, a reference basis is provided for identifying the wheat variety with the low grain protein content. According to the primer, the kit and the method, the low grain protein content can be screened at the second breeding and hybridization generation, therefore, the defect that in the conventional methods, the low grain protein content only can be screened at the fourth hybridization generation is overcome, thus the working amount of breeders for selecting the characters is reduced, the selecting accuracy and breeding efficiency are improved, and the technology belongs to the novel breeding technology which is efficient and rapid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a primer, a kit, a detection method and an application thereof for detecting low grain protein content of wheat. Background technique [0002] With the continuous increase of my country's wheat production, people's demand for high-quality wheat is increasing. According to the quality of wheat, my country divides wheat into strong gluten wheat, medium gluten wheat and weak gluten wheat. Weak gluten wheat is mainly used for the production of biscuits, pastries and For flour products such as southern steamed buns, wheat grain protein content (GPC) is an important indicator of quality. Wheat varieties with low grain protein content can be used as raw materials for the production of high-quality weak-gluten wheat products. my country’s national standard for high-quality weak-gluten wheat (GB / T17893- 1999) stipulates that the crude protein content of wheat grain shall not exceed ...

AI Technical Summary

Problems solved by technology

Blanco et al. (2002) discovered seven QTL loci controlling grain protein content, located on chromosomes 4B, 5A, 6A, 6B, 7A, and 7B, respectively; Prasad et al. (1999) located the QTL loci controlling grain protein content in On chromosome 2D; Huang et al. (2006) used doubled haploid populations to find QTLs controlling grain protein content on chromosomes 4B and 4D; Li et al. (2007) used two recombinant inbred lines containing 229 and 485 families 3 and 10 QTLs affecting grain protein content were detected in the population, respectively located in 2B, 3A, 4A, 4D, 5B, 7A, 7B and 1A, 1B, 2A, 2D, 3A, 4B, 5A, 5D, 6B , 7D chromosome, according to the current public literature, there are many genes or QTLs that control the grain protein content, but the single effect value is not high, and the conventional method can only check the grain protein content after the 4th generation of hybridization , thus increasing the workload of breeders to select this trait, and the accuracy and breeding efficiency are low

Images