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Gene chip and kit used for detecting foot and mouth disease viruses, vesicular stomatitis viruses and swine vesicular disease viruses

A technology for porcine vesicular disease virus and foot-and-mouth disease virus, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and microorganism measurement/inspection. high effect

Inactive Publication Date: 2015-11-04
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the diagnosis of these three diseases mostly adopts isolation and identification and conventional serological methods, which takes a long time. There is also a method for identification by PCR technology, but there is no method that is highly operable and can simultaneously identify the three viruses.

Method used

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  • Gene chip and kit used for detecting foot and mouth disease viruses, vesicular stomatitis viruses and swine vesicular disease viruses
  • Gene chip and kit used for detecting foot and mouth disease viruses, vesicular stomatitis viruses and swine vesicular disease viruses
  • Gene chip and kit used for detecting foot and mouth disease viruses, vesicular stomatitis viruses and swine vesicular disease viruses

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0056] Example 1 Detection method of the present invention

[0057] 1. Materials and instruments

[0058] Same as the aforementioned experimental materials and instruments.

[0059] 2. Experimental method

[0060] 2.1 Design and synthesis of PCR primers and detection probes

[0061] 2.1.1 Primer design:

[0062] According to the FMDV-O type sequence, FMDV-A type sequence, FMDV-Asia type 1 sequence, VSV sequence and SVDV sequence published by NCBI, on the basis of fully comparing the homology, the conserved regions are selected and the primers are designed with the software Primer Primer 5.0 And conduct BLAST sequence alignment to initially verify the primer specificity. The primers used in this study are shown in Table 1. Each pair of primers has a Cy3 fluorophore attached to the 5'end of the upstream primer. All primers were synthesized by Shenggong Bioengineering (Shanghai) Company.

[0063] Table 1 Primer sequence and target amplification product size

[0064]

[0065] 2.1.2 Probe de...

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Abstract

The invention discloses a gene chip and kit used for detecting foot and mouth disease viruses, vesicular stomatitis viruses and swine vesicular disease viruses. The gene chip and kit and a detection method provided by the invention can accurately and effectively detect foot and mouth disease viruses, vesicular stomatitis viruses and swine vesicular disease viruses and have the advantages of high specificity and sensitivity, short detection time, rapid detection and good application prospects.

Description

Technical field [0001] The invention relates to a gene chip and a detection method for detecting foot-and-mouth disease virus, vesicular stomatitis virus and swine vesicular disease virus. Background technique [0002] Foot-and-mouth disease (FMD), swine vesicular disease (SVD), and vesicular stomatitis (VS) are caused by foot-and-mouth disease virus (FMDV), Swine vesicular disease virus (SVDV) and Vesicular stomatitis virus (Vesicular stomatitis virus, VSV) cause acute and highly contact infectious diseases in mammals. These three types of viruses can infect pigs and cause disease. The rate is extremely high, and can form a large-scale epidemic, can cause serious public health problems, and are listed as legally reported animal diseases by the World Organization for Animal Health (OIE). The main symptoms of these three diseases are the occurrence of blisters on the epithelium of pig tongue, lips, oral mucosa, nipples and hoof crowns. Therefore, the clinical symptoms cannot be d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
CPCC12Q1/701
Inventor 文心田黄小波欧阳达曹三杰文翼平伍锐
Owner SICHUAN AGRI UNIV
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