Unlock instant, AI-driven research and patent intelligence for your innovation.
Visual gene chip and kit for porcine pseudorabies virus and/or porcine circovirus type 2
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technique for porcine pseudorabies virus and porcine circovirus, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and microorganism determination/inspection, etc. Long, observable and intuitive effect
Active Publication Date: 2017-11-07
SICHUAN AGRI UNIV
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0003] At present, the diagnosis of these two diseases mostly adopts isolation and identification and conventional serological methods, which takes a long time. There is also a method for identification by PCR technology, but there is no method that is highly operable and can simultaneously identify the two viruses.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0027] Embodiment 1 Detection method of the present invention
[0028] 1. Design of probe genes and construction of plasmidbacteria containing probes
[0029] 1 material
[0030] 1.1 kind of poison
[0031] PRV Fa strain, donated by Chengdu Institute of Biological Products, Ministry of Health;
[0032] PCV2 was isolated from Ziyang disease materials in Sichuan by Zhao Chunrong in our laboratory;
[0033] Inactivated porcine parvovirus vaccine was purchased from Beijing Zhonghai Science and Technology Animal Health Technology Company.
[0039] Ultra-clean bench: SW-CJ-2FD, Suzhou Antai Air Technology Co., Ltd.;
[0040] Constant temperature shaker: vacuum machine: SinBo, Hong Kong;
[0041] Constant t...
Embodiment 2
[0160] Example 2 Using the visualized gene chip and kit of the present invention to detect actual samples
[0161] 1. Clinical samples: Spleen, lung, liver, kidney, lymph nodes and other tissues collected from pigs suspected of porcine reproductive disorders in Chengdu, Guang'an, Leshan, Deyang, Anyue, Panzhihua and other places in Sichuan.
[0162] 2. Detection method
[0163] The lesions of the spleen, lung, liver, kidney, and lymph nodes were collected from 103 clinical samples in Sichuan, Chongqing, Fujian, Guizhou, Gansu, etc., and the virus genome was extracted using the Tiangen Blood / Cell / Tissue Genomic DNA Extraction Kit, according to the examples 1 method for detection.
[0164] 3. Test results
[0165] The results are shown in the table below:
[0166] Table 1: Test results of clinical samples
[0167]
[0168] The positive rates of PRV and PCV2 were 22.3% (2.9% for the vaccine strain and 19.4% for the wild strain) and 78.6% respectively, and 22.3% for PRV+PCV...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses gene chips for detecting porcine pseudorabiesvirus and / or porcine circovirus type 2. The gene chips comprise solid-phase carriers and probes fixed on the solid-phase carriers, wherein the probes comprises gene segments shown as SEQ ID NO:1 and / or 3, and used for detecting the porcine pseudorabiesvirus and / or the porcine circovirus type 2. The invention further discloses a kit for detecting the porcine pseudorabiesvirus and / or the porcine circovirus type 2. The gene chips and the kits can be used for effectively detecting the porcine pseudorabies virus and / or the porcine circovirus type 2, and distinguishing the infection of porcine pseudorabies virus vaccine strains from that of porcine pseudorabies virus wild strains, and has the advantages of being high in specificity and sensitivity, and long in storage life; moreover, the time consumption is low, the detection is fast, the detection results can be visibly observed, no expensive equipment is needed, the infection situation of livestock and poultry plague can be rapidly mastered, and the clinical application prospect is good.
Description
technical field [0001] The invention relates to a visual gene chip and a detection method for detecting porcine pseudorabies virus and / or porcine circovirus type 2. Background technique [0002] With the development of my country's pig industry in the direction of intensification and scale, large-scale pig farms have frequent epidemics and mixed infections of various pathogens, which have caused certain losses to the pig industry. Among them, porcine pseudorabies virus (PRV) and porcine circovirus type 2 (PCV2) are two important disease pathogens that cause reproductive disorders in pigs. The clinical symptoms of these two diseases are similar, and a differential diagnosis is required. [0003] At present, the diagnosis of these two diseases mostly adopts isolation and identification and conventional serological methods, which take a long time. There is also a method for identification by PCR technology, but there is no method that is highly operable and can simultaneously ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More