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Preparation method of composite feed additive and anti-virus agent and application of anti-virus agent

A technology of compound feed and antiviral agent, applied in the direction of antiviral agent, application, animal feed, etc., can solve the problem that there is no safe and effective vaccine prevention and control, achieve rich nutritional components and live bacteria, and enhance disease resistance power, prevent infection

Inactive Publication Date: 2018-03-23
河南邑鸿善成生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Due to the widespread prevalence, high infectivity, variability, and the particularity of antibody protection of VSV, there is currently no safe and effective vaccine for prevention and control.

Method used

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  • Preparation method of composite feed additive and anti-virus agent and application of anti-virus agent
  • Preparation method of composite feed additive and anti-virus agent and application of anti-virus agent
  • Preparation method of composite feed additive and anti-virus agent and application of anti-virus agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Preparation of Saccharomyces cerevisiae fermentation broth

[0040] 1) Preparation of Saccharomyces cerevisiae seed liquid: Inoculate Saccharomyces cerevisiae into S. cerevisiae liquid seed culture medium at 30 °C and cultivate at 180 r / min for 20 h to obtain S. cerevisiae seed liquid.

[0041] 2) Preparation of Saccharomyces cerevisiae fermentation broth: inoculate the Saccharomyces cerevisiae seed liquid into the Saccharomyces cerevisiae liquid fermentation medium in the fermenter according to the inoculum amount of 5%, and enlarge the culture, the air flow rate is 30 m3 / h, and the stirring speed is 150 r / min , the temperature was 30 °C, and the culture time was 18 h to obtain the fermentation liquid of Saccharomyces cerevisiae. At this point, the number of yeast ≥ 3.0 × 10 8 CFU / mL.

[0042] in,

[0043] The composition of Saccharomyces cerevisiae liquid seed medium is: tryptone 20 g / L, anhydrous glucose 20 g / L, yeast extract powder 5 g / L, pH 7.0;

[0044] Th...

Embodiment 2

[0057] One, the preparation of antiviral agent

[0058] Weigh 1 g of the compound feed additive in Example 1, add 2 ml of triple-distilled water, shake in a water bath at 37°C for 30 minutes, then centrifuge at 1000 rpm for 10 minutes, absorb the supernatant, and adjust the pH to 7.0 with 0.22 M NaOH. mu m Filtrate with a sterile filter to prepare the original solution, record the original solution as C1, and dilute it by 4 times, respectively as C2, C3, and C4.

[0059] 2. Culture of African green ape kidney (Vero) cells and VSV virus

[0060] Resuscitate Vero cells, digest Vero cells with 0.25% trypsin, blow and blow to disperse the cells, and use DMEM complete culture medium (containing 2% fetal bovine serum, 10 5 U / L penicillin G potassium salt and 100 g / L streptomycin sulfate, pH 7.2) were cultured in T25 flasks at 37 °C, 5 % CO 2 cultured in an incubator. After culturing for 24 h, most of the cells had adhered to the wall, discarded the culture medium in the bottle...

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Abstract

The invention discloses a preparation method of a composite feed additive and the composite feed additive prepared with the preparation method, further discloses a method for preparing an anti-virus agent from the composite feed additive, and the anti-virus agent, and further discloses application of the anti-virus agent in inhibiting vesicular stomatitis viruses and application in preventing animal cells from infection of the vesicular stomatitis viruses. The invention has the beneficial effects that 1, due to mixing and fermentation of two bacteria, namely brewer's yeast and methyl nutritional type bacillus, the composite feed additive is relatively rich in nutrient component and active bacterium, is capable of greatly improving the disease resistance of animals, and has great significances in livestock feeding and feed industry; 2, the anti-virus agent is a composite feed additive lixivium, and has remarkable activity and proliferation inhibition upon VSVs (Vesicular Stomatitis Viruses); 3, the anti-virus agent is capable of effectively preventing animal cells from VSV infection.

Description

technical field [0001] The invention relates to a preparation method of a compound feed additive and the compound feed additive prepared by the preparation method, and also relates to an antiviral agent prepared by using the compound feed additive and the application of the antiviral agent, belonging to the field of biotechnology. Background technique [0002] The aquaculture industry is developing rapidly in today's society, but at the same time, there are many problems in the extensive use of antibiotics. If antibiotics are used for too long, it is easy for pathogenic bacteria to develop drug resistance. Moreover, if antibiotics are very easy to remain in the body, it is easy to affect the immune effect of humans and animals through the food chain, laying hidden dangers for controlling the occurrence of epidemic diseases. At the same time, antibiotics will destroy the micro-ecological balance in the intestinal tract, and at this time, microorganisms outside the body may in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/30A23K10/37A23K10/38A23K20/163A23K20/24A23K20/26A23K20/20A61K33/42A61P31/14A61K33/30A61K33/24A61K33/14A61K33/06A61K33/04A61K31/7004
CPCA23K10/12A23K10/30A23K10/37A23K10/38A23K20/163A23K20/24A23K20/26A23K20/30A61K31/7004A61K33/04A61K33/06A61K33/14A61K33/24A61K33/30A61K33/42A61K2300/00Y02P60/87
Inventor 潘韵陈泽田王逢久仵桂仓郑文官
Owner 河南邑鸿善成生物技术有限公司
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