A kind of test kit for blister venereal disease
A technology for vesicular stomatitis and swine vesicular disease virus, which is applied in the determination/examination of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc. The number of easy-to-contaminate samples, etc., can reduce the cost of analysis, the time of the experiment, the accuracy of the experimental results, the high sensitivity and the repeatability.
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[0039] The present invention will be further described below by embodiment:
[0040] The method in this example is a method for detecting porcine vesicular venereal disease with a GeXP multiplex PCR system. The method mainly consists of 6 pairs of primers, formamide solution, separation buffer, separation gel, viral RNA, PrimrScript One Step EnzymeMix, 2×One Step Buffer and sterile double distilled water.
[0041] According to the complete nucleotide sequences of foot-and-mouth disease virus (type A, type O and Asia 1), porcine vesicular disease virus and vesicular stomatitis virus published by NCBI (GenBank accession number: AY429470), refer to GeXP multiplex PCR system primers According to the design requirements, 6 pairs of primers were designed and synthesized, including 5 pairs of specific chimeric primers and 1 pair of universal primers containing fluorescent tags. The specific primer sequences are as follows:
[0042] Universal Primer-F: Cy5 AGGTGACACTATAGAATA
[0043...
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