36results about How to "Avoid amplification" patented technology

The invention discloses a near-field acoustical holography method based on a combinatorial optimization regularization method. The sound pressure at each point measured on a holographic surface is used, based on a statistical optimal cylinder near-field acoustical holography method, the truncated singular value and standard Tikhonov combinatorial optimization regularization method are used for selected wave number vectors to suppress measurement errors of the wave number vectors caused by the noise and random errors, and that is the combinatorial optimization regularization method; by using the combinatorial optimization regularization method and a GCV method, a superposition coefficient matrix and the sound pressure at each point measured on the holographic surface are obtained, and a sound field on the surface of shell structure equipment can be obtained by expressing the sound pressure at each point on the surface of the shell structure equipment as linear superposition of the conformal measurement surface sound pressure; and effectiveness of the near-field acoustical holography method in shell radiation noise analysis is fully demonstrated, the near-field acoustical holographymethod is suitable for the cylindrical shell structure, a sound field of a cylindrical underwater vehicle is displayed visually, and thus the magnitude and distribution of a radiated sound field can be seen intuitively, so that important theoretical significance and engineering application values are achieved.

The invention discloses establishment and application of a novel HIV-1 drug resistance detection method. The novel HIV-1 drug resistance detection method established according to the invention has theadvantages that 1, by utilizing multiple sets of primer combinations, a problem of low amplification efficiency of target genes, which is caused by differences of different subtypes of strain sequences, is considered; and 2, synthesis of PR, RT and IN target gene cDNA and first-round amplification of a target gene nested PCR are completed in one reaction tube so as to avoid respective amplification on the target genes, simplify the experimental process and save reagent cost by 50%. The detection method established according to the invention can simultaneously cover multiple strain subtypes, and simultaneously cover multiple gene regions, which enables HIV-1 drug resistance detection efficiency to be greatly improved, and has the great influence on aids prevention and public health.

The invention provides a 3D hand-eye calibration method and device for a mobile robot. The method comprises the following steps: S1, calibrating the view finding range of a camera to cover all degrees of freedom of the mobile robot; S2, recording point cloud data of a calibration ball held by the mobile robot in a camera coordinate system and a robot pose corresponding to the calibration ball in a current robot coordinate system to form n groups of point cloud data; S3, extracting the point cloud of the spherical surface of the calibration ball, and determining the coordinate value of the center point of the calibration ball in the camera coordinate system in each group of point cloud data by adopting a least square fitting algorithm; S4, solving offset and a hand-eye calibration matrix according to a first algorithm according to the pose of the robot and the coordinates of the center point of the corresponding calibration ball; and S5, converting the hand-eye calibration matrix into an Euler angle form, and taking a hand-eye calibration matrix result as an initial value of nonlinear optimization; and respectively calculating a hand-eye calibration matrix and a difference value between the offset and the truth value according to a second algorithm, and correcting the hand-eye calibration matrix according to the difference value. Therefore, the adaptation requirement for the degree of freedom of the robot is lowered, and the universality is improved.

The invention discloses a construction method of a gene detection library of hereditary hypertrophic cardiomyopathy and a kit and relates to mutation of ACTC1, ACTN2, MYBPC3, MYH7, MYL2, MYL3, TNNI3,TNNT2 and TPM1 genes. Target regions comprise an exon region of coding amino acid of the 9 genes and 20 basic group regions at the upstream and downstream of an exon; in order to ensure that all the target regions are covered, a target region library is obtained through a hybridization probe capturing method after the library is prepared; then an LMPCR (Ligation-Mediated Polymerase Chain Reaction)method is adopted to amplify the library; and after the library is purified, a sample library for sequencing is obtained. The library construction method has simple steps and a rapid speed and the cost of constructing the library is effectively reduced; related genes of the hypertrophic cardiomyopathy are covered; an illumina high-throughput sequencing machine is combined and related gene mutation can be rapidly and accurately obtained; and the construction method has great significance on the hereditary hypertrophic cardiomyopathy.

The invention discloses a flash photography object regularization reconstruction method based on a truncated singular value and total variation. The flash photography object regularization reconstruction method includes: (1) obtaining flash photography imaging negative film information through a forward-direction photon transport program; (2) obtaining spatial distribution and geometric parameters of flash photography object linear coefficients through a truncated singular value method; and (3) obtaining the spatial distribution and the geometric parameters of the flash photography object linear coefficients through a total variation regularization method. The flash photography object regularization reconstruction method can effectively reconstruct flash photography object linear absorption coefficients, has high calculation accuracy, has clear resolution at junction of object internal materials simultaneously, has high edge fidelity ability, and meets engineering requirements.

The invention discloses a constructing method of a genetic testing library for inherited arrhythmias and a kit for inherited arrhythmias, and relates to mutation of KCNH2, KCNQ1, SCN5A, RYR2, CASQ2, TRDN and CALM1 genes. Target regions are exon regions of coded amino acid of the seven genes, 20 base regions on the upstream of an exon and 20 base regions on the downstream of the exon; for ensuringall coverage of the target regions, after a preparation library is adopted, a target region library is obtained by a hybridization probe capturing method; then the library is amplified by adopting anLMPCR method; the library is purified to obtain a sample library for sequencing. The constructing method comprises simple and quick steps, so that the constructing cost of the library is effectively reduced; in addition, related genes of arrhythmia are covered; by combination with an illumine high throughput sequencer, the mutation of the related genes can be quickly and accurately obtained; the constructing method and the kit have important significance for the inherited arrhythmias.

The invention discloses a gene detection library construction method and a kit for hereditary dilated cardiomyopathy, and relates to mutation of ANKRD1, BAG3, LDB3, LMNA, MYBPC3, MYH6, MYH7, RBM20, SCN5A, TCAP, TNNC1, TNNI3, TNNT2, TPM1 , TTN and VCL genes. Target regions include exon regions of coded amino acids of the 16 genes and 20 basic group regions of each of the upstream and downstream ofeach exon; in order to ensure the full coverage of the target regions, a target region liberty is acquired according to a hybridization probe capture method after library preparation and then amplified by the LMPCR (ligation mediated polymerase chain reaction) method, and a sample library for sequencing is acquired after library purification. The library construction method has the advantages thatthe steps are simple and fast, the library construction cost is reduced effectively, arrhythmia related genes are included, related gene mutations can be acquired quickly and accurately through an Illumina high-throughput sequencer, and accordingly, the library construction method is important for the hereditary dilated cardiomyopathy.

The invention discloses a detection method and a detection kit for PKD1 gene and PKD2 gene mutation. Amplification is performed by creatively designing multiple pairs of specific long fragment PCR primers, and when amplification is performed by adopting the human genome DNA as a template, amplification of pseudogenes with high homology can be avoided, and therefore the appearance of the false positive and false negative result brought by the pseudogenes can be avoided, and the detection accuracy of mutation of the PKD1 gene and PKD2 gene can be greatly improved. A high-throughput sequencing technique is combined, and the method and the kit when compared with a traditional medical exon trapping technique, can simplify a technical process, reduce the detection cost and shorten the detectionperiod.

The embodiment of the invention provides a gravity abnormal boundary enhancement method and device. The method comprises the steps of: obtaining gravity abnormal data of a target work area, and meshing the gravity abnormal data; adopting a space domain operator to extract the gravity abnormal boundary extremum information of a first grid mesh point in a preset direction one by one; and based on the abnormal boundary extremum information of the first mesh point, calculating a coherent enhancement value of a second mesh point. According to the invention, space domain processing is carried out on the gravity abnormal data, the amplification effect of a conventional frequency domain algorithm on data noise is avoided, and the clear and continuous gravity abnormal boundary information can be obtained.

The invention provides a method for building a buckwheat AFLP reaction system. According to the method for building the buckwheat AFLP reaction system, reaction conditions of AFLP critical steps are improved, and the number of extraction times is increased properly, so that not only are impurities in reaction liquid removed fully, but also mechanical breakage of buckwheat DNA is reduced and quality of extraction of the DNA is improved; digestion is fully performed on buckwheat genome DNA for the set digestion time; the set Mg2+ concentration can improve the output rate of product specific DNA and avoid amplification of non-specific DNA, the reaction system which is good in reliability, strong in repeatability, high in credibility, convenient and quick to achieve and suitable for buckwheat is built, few DNA samples are needed, Southern hybridization is not needed, and serial information of the DNA does not need to be known in advance. The method is simple in process and easy to operate, the output amount is high, and production cost is lowered.

The invention discloses a method for preparing a hot start Taq polymerase. The method comprises the following step of mixing the Taq polymerase with a a treatment solution, so as to obtain the hot start Taq polymerase, wherein the treatment solution is a heparin solution, a heparin sodium solution or a heparin potassium solution. The method for preparing the hot start Taq polymerase has the beneficial effects that the hot start can be realized in the first step of PCR (Polymerase Chain Reaction) reaction during carrying out the PCR reaction, and the hot start also can be realized in the follow-up steps of the PCR reaction, so that the amplification of non-specific sequences can be effectively avoided, and the reaction specificity, reliability, uniformity and sensitivity of the DNA (Deoxyribose Nucleic Acid) polymerase can be improved; compared with the conventional similar product, the hot start Taq polymerase prepared by the method disclosed by the invention has the advantages that the amplification efficiency is increased, the hot start Taq polymerase can be detected from a micro sample and trace sample under relatively low cycle number as well as be cloned to a target gene, thus the personal error can be improved to a great extent; and the hot start Taq polymerase is suitable for being applied to amplification and detection of general PCR reaction, complex templates and trace templates.

The present invention relates to multiplexed methods of detecting an analyte in a sample using two or more padlock probes each specific to a different target sequence, the target sequence being eitherpart of an analyte or indicative of the presence of an analyte in the sample, wherein each padlock probe comprises an analyte-specific reporter sequence, and either a restriction cleavage site located 3' of the analyte-specific reporter sequence, and / or a first amplification primer binding site for an amplification reaction, wherein where the padlock probe comprises a restriction cleavage site, cleavage at said restriction cleavage site occurs 3' of the analyte-specific reporter sequence, and where the padlock probe comprises a first amplification primer binding site for the further amplification reaction, it does not contain a second amplification primer binding site 5' of the analyte-specific reporter sequence, and panels of probes and kits for the same.

The invention belongs to the technical field of automobile suspensions, and particularly discloses an active and passive combined self-adaptive vibration control suspension system and a control method. The system comprises a suspension passive module, a suspension active module and a control module which are sequentially arranged from top to bottom, the suspension passive module inputs vibration needing to be attenuated, and the suspension active module comprises a first elastic element, an electromagnet module and a second elastic element. The control module is used for controlling the suspension active module to output corresponding exciting force by adopting a feedforward and feedback combined mode so as to further eliminate the vibration of the suspension passive end after primary passive vibration isolation. According to the method, a mode of combining feedforward and feedback is triggered through a reference signal with or without a vibration source, so that exciting force used for counteracting an actual vibration signal is obtained. An active and passive composite suspension structure is used for eliminating vibration of the active suspension end, and the system has the advantages of being simple in structure, good in robustness and high in adaptability.

The invention relates to the technical field of digital X-ray imaging methods, and discloses an imaging device through slit scanning. The imaging device comprises a transmitting end and a receiving end, wherein the transmitting end is used for collimating X-rays into slit X-rays, continuously transmitting the slit X-rays to an object to be imaged, and completely shooting the object to be imaged by the slit X-rays in a linear motion surface forming mode; and the receiving end is used for automatically activating a slit region of interest corresponding to the transmitting direction of the slit X-rays on the receiving end after the slit X-rays transmitted each time pass through the object to be imaged, acquiring attenuated slit X-rays, converting the slit X-rays into slit images with the same shooting range as the slit X-rays for storage, and splicing the slit images after complete shooting. The X-rays are collimated in a slit scanning mode, so that the amplification effect of point light source imaging is avoided; the imaging device can be used for acquiring images of a patient in a real proportion; and the radiation dose of the patient is far lower than the dose of spiral scanning, and is at the level of common radiation.

The invention belongs to the technical field of biochemical analysis, and discloses a kit for predicting the drug resistance of gastrointestinal stromal tumours, and application thereof. The kit comprises a PCR reaction component, a PCR product purification component, a PCR sequencing component sealing plate coating film and a product specification; and an application method of the kit for predicting the drug resistance of the gastrointestinal stromal tumours comprises the following steps of: performing venous blood extraction on a gastrointestinal stromal tumour patient taking the drug; extracting genome DNA by using a phenol chloroform method; setting a reaction system and reaction conditions for PCR amplification, purification and sequencing, and performing PCR sequencing after the reaction is finished; and, analyzing the free tumour cell condition of the gastrointestinal stromal tumour of the gastrointestinal stromal tumour patient taking the drug, so that a conclusion whether thedrug influences the gastrointestinal stromal tumour or not is obtained. The kit provided by the invention is reasonable in design, and can realize accurate acquisition of free tumour cells of the gastrointestinal stromal tumour; therefore, a conclusion whether the drug resistance of the gastrointestinal stromal tumour resists or not is obtained; and furthermore, the kit is convenient to use and low in cost.

The invention relates to the technical field of biology, in particular to an alcohol-free lysate suitable for nucleic acid extraction of various samples and a nucleic acid extraction kit. The invention provides an alcohol-free lysate and a kit. The alcohol-free lysate comprises cetearyl alcohol polyether, alkyl glycoside and a reducing agent component. Compared with an existing alcohol-free nucleic acid extraction reagent, the method has the advantages that the concentration of extracted nucleic acid is high, experimental steps are simple and convenient, consumed time is short, alcohol reagents are not contained, the detection performance is good, the method can be combined with an automatic platform, full-automatic randomized simultaneous detection of multiple sample types is achieved, and the method has a wider application prospect.

The invention discloses a wind turbine dynamic virtual inertia control strategy and system based on a tracking differentiator. The wind turbine dynamic virtual inertia control strategy comprises the following steps: acquiring a frequency deviation signal of a power system; according to the frequency deviation signal, tracking filtering is carried out through a preset tracking differentiator to obtain a first state signal and a second state signal, the first state signal and the second state signal are used as feedback input signals of the tracking differentiator, the first state signal is tracking state frequency deviation, and the second state signal is a frequency change rate; determining a control reference power signal through a preset virtual inertia controller according to the first state signal and the second state signal; according to a control reference power signal, output power compensation is carried out on a wind power plant where a fan is located, the amplification effect of a conventional differentiator on a high-frequency signal is avoided, measurement noise and high-frequency interference are effectively suppressed, wind power participates in frequency modulation in combination with a dynamic virtual inertia control method, releasable kinetic energy of the fan is more fully utilized, and the measurement efficiency is improved. And the wind power frequency modulation participation capability is improved.

The invention provides a primer group for nest type RT-PCR detection of seneca valley virus (SVV), a kit and application and belongs to the technical field of molecular biological detection of viruses. Two pairs of specific primers are designed according to an SVV gene sequence, first round of RT-PCR amplification reaction is performed with cDNA of extracted RNA after reverse transcription as a template, second round of RT-PCR amplification reaction is performed with a first round of RT-PCR amplification product as a template, the amplification product is detected by agarose gel electrophoresis, if specific target fragments with size of 523bp exist, the detected virus is determined as the SVV. The primer group detects SVV by the nest type RT-PCR technology, has the advantages of high specificity, high sensitivity and short detection time, can meet requirements of enterprises for production research and development and agricultural production and provides a new thought for monitoring and control of the SVV.

The invention relates to a nested PCR (Polymerase Chain Reaction) detection kit for canna yellow mottle virus and a detection method thereof, which are specially used for detecting the canna yellow mottle virus. The kit comprises an outer-side upstream primer, an outer-side downstream primer, an inner-side upstream primer, an inner-side downstream primer, PCR Buffer, dNTPs, Mgcl2, Taq DNA polymerase, positive control, negative control and ddH2O. According to the invention, two pairs of specific primers are designed according to the gene sequence of the canna yellow mottle virus, extracted DNA is adopted as a template to carry out first-round PCR amplification, first-round PCR products are adopted as a template to carry out second-round PCR amplification, amplification products are detected by gel electrophoresis of agarose, and the detected virus is judged to be the canna yellow mottle virus if specific target fragments with the size being 325bp occur. The kit detects the canna yellow mottle virus by utilizing a nested PCR technology, has the advantages of strong specificity, high sensitivity and short detection time, and can meet the need of entry and exit port quarantine and agricultural production.

The invention discloses a detection method for accurately quantifying HIV DNA in cooperation with multiple PCRs. Particularly, a nested PCR, a real-time fluorescence quantification PCR and a Digital PCR are used in a combined mode to accurately quantify the HIV DNA; through combination of the three PCR methods, specific primers for all the subtypes of HIV are designed, and the aims of accurate quantification and cost saving of the HIV DNA are achieved; in addition, a detection kit for accurately quantifying the HIV DNA according to the detection method is developed, and the detection kit has broad application prospects in clinic.

The invention relates to a nested PCR (Polymerase Chain Reaction) detection kit for canna yellow mottle virus and a detection method thereof, which are specially used for detecting the canna yellow mottle virus. The kit comprises an outer-side upstream primer, an outer-side downstream primer, an inner-side upstream primer, an inner-side downstream primer, PCR Buffer, dNTPs, Mgcl2, Taq DNA polymerase, positive control, negative control and ddH2O. According to the invention, two pairs of specific primers are designed according to the gene sequence of the canna yellow mottle virus, extracted DNA is adopted as a template to carry out first-round PCR amplification, first-round PCR products are adopted as a template to carry out second-round PCR amplification, amplification products are detected by gel electrophoresis of agarose, and the detected virus is judged to be the canna yellow mottle virus if specific target fragments with the size being 325bp occur. The kit detects the canna yellow mottle virus by utilizing a nested PCR technology, has the advantages of strong specificity, high sensitivity and short detection time, and can meet the need of entry and exit port quarantine and agricultural production.

The invention discloses a near-field acoustical holography method based on a combinatorial optimization regularization method. The sound pressure at each point measured on a holographic surface is used, based on a statistical optimal cylinder near-field acoustical holography method, the truncated singular value and standard Tikhonov combinatorial optimization regularization method are used for selected wave number vectors to suppress measurement errors of the wave number vectors caused by the noise and random errors, and that is the combinatorial optimization regularization method; by using the combinatorial optimization regularization method and a GCV method, a superposition coefficient matrix and the sound pressure at each point measured on the holographic surface are obtained, and a sound field on the surface of shell structure equipment can be obtained by expressing the sound pressure at each point on the surface of the shell structure equipment as linear superposition of the conformal measurement surface sound pressure; and effectiveness of the near-field acoustical holography method in shell radiation noise analysis is fully demonstrated, the near-field acoustical holographymethod is suitable for the cylindrical shell structure, a sound field of a cylindrical underwater vehicle is displayed visually, and thus the magnitude and distribution of a radiated sound field can be seen intuitively, so that important theoretical significance and engineering application values are achieved.

The invention relates to a kit for noninvasive prenatal diagnosis of pregnant woman fetal trisomy syndrome. The kit comprises the following ingredients including an upstream and downstream primer pair PP trisomy designed by aiming one or several of No.21, No.18 or No.13 chromosomes, and an upstream and downstream primer pair PP autosome designed by aiming at any one autosome except the No.21, No.18 or No.13 chromosomes, wherein any one pair of PP trisomy and PP autosome needs to meet the following conditions that 1, the melting point difference values between every two primers of the four primers is within 4 DEG C; 2, the amplicon length is smaller than or equal to 150 bp and a non-target product is not generated; 3, the melting point difference value of the two amplicons is 2 DEG C or higher; 4, the melting peaks of the two amplicons are obviously separated, so the shoulder peak is not formed. The kit has the advantages that simplicity, convenience and high speed are realized; the accuracy and the sensitivity are high; the errors are small; the precision is high, so that the effective Down symdrome screening gestational week period is shortened by at least three weeks.

The present invention discloses a fresh air module and an air conditioner, wherein the fresh air module comprises a shell, a fresh air duct is formed in the shell, a fan assembly comprises a motor and a wind wheel, the wind wheel is arranged in the fresh air duct, the motor comprises a motor body and a motor shaft, the motor shaft is connected to the wind wheel drivingly, so as to drive the wind wheel to rotate and guide the airflow to flow through the fresh air duct, the motor cover is arranged in the shell, and is located outside the fresh air duct, a motor cavity is formed in the motor cover, the motor body is arranged in the motor cavity, wherein a partition is provided between the motor cavity and the fresh air duct, so that the motor cavity and the fresh air duct are separated from each other, the motor shaft is passed through the partition, and is connected to the wind wheel drivingly. The fresh air module provided by the present invention has a motor body arranged outside the fresh air duct, and is isolated from the fresh air duct by the partition, so as to avoid the amplification effect of the wind cavity on the noise, thereby reducing the noise generated by the fresh air module.

A method for preparing hot start Taq polymerase comprises mixing the Taq polymerase with treatment solution to obtain the hot start Taq polymerase, wherein the treatment solution is heparin solution, heparin sodium solution or heparin potassium solution.

The invention relates to the microelectronic technology field. The invention discloses an anti-single-event transient reinforcement SOI member, comprising a substrate, a buried oxide layer, a semiconductor body region, a drain region, a source region, a grid region, gridside walls, LDD regions and a heavily doped source extending region; the buried oxide layer is arranged above the substrate; the semiconductor body region, the source region and the drain region are arranged above the buried oxide layer; the semiconductor body region is positioned between the source region and the drain region; the LDD regions are positioned on two side ends of the semiconductor body region and in contact with the source region and the drain region; the grid region is positioned above the semiconductor body region; the two side gridside walls are arranged on two sides of the grid region and above the LDD regions; the source extending region is arranged among the source region, the semiconductor body region and the buried oxide layer; and the doping types of the source extending region and the source region are opposite. The invention also discloses a manufacture method for the anti-single-event transient reinforcement SOI member. The anti-single-event transient reinforcement SOI member and the manufacture method for the same can effectively inhibit SOI member single-event overturning and the single-event transient effect which are caused by single-event radiation, and the technology is simple and compatible with the current technology.