33results about How to "Avoid amplification" patented technology

The invention discloses establishment and application of a novel HIV-1 drug resistance detection method. The novel HIV-1 drug resistance detection method established according to the invention has theadvantages that 1, by utilizing multiple sets of primer combinations, a problem of low amplification efficiency of target genes, which is caused by differences of different subtypes of strain sequences, is considered; and 2, synthesis of PR, RT and IN target gene cDNA and first-round amplification of a target gene nested PCR are completed in one reaction tube so as to avoid respective amplification on the target genes, simplify the experimental process and save reagent cost by 50%. The detection method established according to the invention can simultaneously cover multiple strain subtypes, and simultaneously cover multiple gene regions, which enables HIV-1 drug resistance detection efficiency to be greatly improved, and has the great influence on aids prevention and public health.

The invention provides a 3D hand-eye calibration method and device for a mobile robot. The method comprises the following steps: S1, calibrating the view finding range of a camera to cover all degrees of freedom of the mobile robot; S2, recording point cloud data of a calibration ball held by the mobile robot in a camera coordinate system and a robot pose corresponding to the calibration ball in a current robot coordinate system to form n groups of point cloud data; S3, extracting the point cloud of the spherical surface of the calibration ball, and determining the coordinate value of the center point of the calibration ball in the camera coordinate system in each group of point cloud data by adopting a least square fitting algorithm; S4, solving offset and a hand-eye calibration matrix according to a first algorithm according to the pose of the robot and the coordinates of the center point of the corresponding calibration ball; and S5, converting the hand-eye calibration matrix into an Euler angle form, and taking a hand-eye calibration matrix result as an initial value of nonlinear optimization; and respectively calculating a hand-eye calibration matrix and a difference value between the offset and the truth value according to a second algorithm, and correcting the hand-eye calibration matrix according to the difference value. Therefore, the adaptation requirement for the degree of freedom of the robot is lowered, and the universality is improved.

The invention discloses a construction method of a gene detection library of hereditary hypertrophic cardiomyopathy and a kit and relates to mutation of ACTC1, ACTN2, MYBPC3, MYH7, MYL2, MYL3, TNNI3,TNNT2 and TPM1 genes. Target regions comprise an exon region of coding amino acid of the 9 genes and 20 basic group regions at the upstream and downstream of an exon; in order to ensure that all the target regions are covered, a target region library is obtained through a hybridization probe capturing method after the library is prepared; then an LMPCR (Ligation-Mediated Polymerase Chain Reaction)method is adopted to amplify the library; and after the library is purified, a sample library for sequencing is obtained. The library construction method has simple steps and a rapid speed and the cost of constructing the library is effectively reduced; related genes of the hypertrophic cardiomyopathy are covered; an illumina high-throughput sequencing machine is combined and related gene mutation can be rapidly and accurately obtained; and the construction method has great significance on the hereditary hypertrophic cardiomyopathy.

The invention discloses a gene detection library construction method and a kit for hereditary dilated cardiomyopathy, and relates to mutation of ANKRD1, BAG3, LDB3, LMNA, MYBPC3, MYH6, MYH7, RBM20, SCN5A, TCAP, TNNC1, TNNI3, TNNT2, TPM1 , TTN and VCL genes. Target regions include exon regions of coded amino acids of the 16 genes and 20 basic group regions of each of the upstream and downstream ofeach exon; in order to ensure the full coverage of the target regions, a target region liberty is acquired according to a hybridization probe capture method after library preparation and then amplified by the LMPCR (ligation mediated polymerase chain reaction) method, and a sample library for sequencing is acquired after library purification. The library construction method has the advantages thatthe steps are simple and fast, the library construction cost is reduced effectively, arrhythmia related genes are included, related gene mutations can be acquired quickly and accurately through an Illumina high-throughput sequencer, and accordingly, the library construction method is important for the hereditary dilated cardiomyopathy.

The invention discloses a detection method and a detection kit for PKD1 gene and PKD2 gene mutation. Amplification is performed by creatively designing multiple pairs of specific long fragment PCR primers, and when amplification is performed by adopting the human genome DNA as a template, amplification of pseudogenes with high homology can be avoided, and therefore the appearance of the false positive and false negative result brought by the pseudogenes can be avoided, and the detection accuracy of mutation of the PKD1 gene and PKD2 gene can be greatly improved. A high-throughput sequencing technique is combined, and the method and the kit when compared with a traditional medical exon trapping technique, can simplify a technical process, reduce the detection cost and shorten the detectionperiod.

The invention provides a method for building a buckwheat AFLP reaction system. According to the method for building the buckwheat AFLP reaction system, reaction conditions of AFLP critical steps are improved, and the number of extraction times is increased properly, so that not only are impurities in reaction liquid removed fully, but also mechanical breakage of buckwheat DNA is reduced and quality of extraction of the DNA is improved; digestion is fully performed on buckwheat genome DNA for the set digestion time; the set Mg2+ concentration can improve the output rate of product specific DNA and avoid amplification of non-specific DNA, the reaction system which is good in reliability, strong in repeatability, high in credibility, convenient and quick to achieve and suitable for buckwheat is built, few DNA samples are needed, Southern hybridization is not needed, and serial information of the DNA does not need to be known in advance. The method is simple in process and easy to operate, the output amount is high, and production cost is lowered.

The invention relates to the technical field of digital X-ray imaging methods, and discloses an imaging device through slit scanning. The imaging device comprises a transmitting end and a receiving end, wherein the transmitting end is used for collimating X-rays into slit X-rays, continuously transmitting the slit X-rays to an object to be imaged, and completely shooting the object to be imaged by the slit X-rays in a linear motion surface forming mode; and the receiving end is used for automatically activating a slit region of interest corresponding to the transmitting direction of the slit X-rays on the receiving end after the slit X-rays transmitted each time pass through the object to be imaged, acquiring attenuated slit X-rays, converting the slit X-rays into slit images with the same shooting range as the slit X-rays for storage, and splicing the slit images after complete shooting. The X-rays are collimated in a slit scanning mode, so that the amplification effect of point light source imaging is avoided; the imaging device can be used for acquiring images of a patient in a real proportion; and the radiation dose of the patient is far lower than the dose of spiral scanning, and is at the level of common radiation.

The invention relates to a nested PCR (Polymerase Chain Reaction) detection kit for canna yellow mottle virus and a detection method thereof, which are specially used for detecting the canna yellow mottle virus. The kit comprises an outer-side upstream primer, an outer-side downstream primer, an inner-side upstream primer, an inner-side downstream primer, PCR Buffer, dNTPs, Mgcl2, Taq DNA polymerase, positive control, negative control and ddH2O. According to the invention, two pairs of specific primers are designed according to the gene sequence of the canna yellow mottle virus, extracted DNA is adopted as a template to carry out first-round PCR amplification, first-round PCR products are adopted as a template to carry out second-round PCR amplification, amplification products are detected by gel electrophoresis of agarose, and the detected virus is judged to be the canna yellow mottle virus if specific target fragments with the size being 325bp occur. The kit detects the canna yellow mottle virus by utilizing a nested PCR technology, has the advantages of strong specificity, high sensitivity and short detection time, and can meet the need of entry and exit port quarantine and agricultural production.

The invention discloses an estimation method and estimator for sideslip angle of straight-line navigation of agricultural machinery, which collect and analyze front wheel steering angle information, forward speed information of agricultural machinery, antenna positioning information and current attitude information. The estimation of sideslip angle is realized based on state observation theory. The first estimator, the second estimator and the third estimator are used to estimate the heading deviation of the vehicle body, position deviation and sideslip angle information. In the analysis process, integration is used instead of differentiation, which avoids the error of amplification by differential operation.

The invention relates to the microelectronic technology field. The invention discloses an anti-single-event transient reinforcement SOI member, comprising a substrate, a buried oxide layer, a semiconductor body region, a drain region, a source region, a grid region, gridside walls, LDD regions and a heavily doped source extending region; the buried oxide layer is arranged above the substrate; the semiconductor body region, the source region and the drain region are arranged above the buried oxide layer; the semiconductor body region is positioned between the source region and the drain region; the LDD regions are positioned on two side ends of the semiconductor body region and in contact with the source region and the drain region; the grid region is positioned above the semiconductor body region; the two side gridside walls are arranged on two sides of the grid region and above the LDD regions; the source extending region is arranged among the source region, the semiconductor body region and the buried oxide layer; and the doping types of the source extending region and the source region are opposite. The invention also discloses a manufacture method for the anti-single-event transient reinforcement SOI member. The anti-single-event transient reinforcement SOI member and the manufacture method for the same can effectively inhibit SOI member single-event overturning and the single-event transient effect which are caused by single-event radiation, and the technology is simple and compatible with the current technology.

The invention discloses a high-strength house anti-loosening fireproof plate with a sound insulation structure, and relates to the technical field of fireproof plate equipment. The high-strength house anti-loosening fireproof plate with the sound insulation structure comprises a positioning frame, a groove ring used for installing a reinforcing frame is formed in the middle of the inner side wall of the positioning frame, a supporting framework mechanism is arranged in an inner cavity of the reinforcing frame, and the supporting framework mechanism is used for enhancing the structural strength in the positioning frame; sound insulation assemblies are arranged on the two side walls of the supporting framework mechanism and used for restraining transmission of various kinds of vibration, and sound frequency in a larger range can be shielded. The two ends of the positioning frame cavity are each provided with a protection plate assembly, the protection plate assemblies are used for further enhancing the structural strength of the positioning frame, the structural strength of the fireproof plate can be enhanced, the sound insulation effect of the fireproof plate can be improved, and meanwhile a user can conveniently recycle and reuse structural materials in the fireproof plate.