19 results about "Multiple strain" patented technology

The invention provides a multi-strain composite fermentation assembly device and a use method, and relates to the technical field of microbial fermentation equipment. The device comprises a bottom fermentation tank, the top end of the bottom fermentation tank is fixedly connected with a fixing opening, the outer wall of the fixing opening is fixedly connected with a material guide opening, the top end of the material guide opening is fixedly connected with a second-stage filter seat, the inner wall of the second-stage filter seat is connected with a second-stage filter screen in a clamped mode, and the inner wall of the second-stage filter screen is fixedly connected with the bottom end of the bottom fermentation tank. A heating fixing structure is arranged at the top end of the bottom end fermentation tank, and a detection material pushing structure is arranged at the top end of the bottom end fermentation tank and located above the heating fixing structure. According to the invention, the dosage of each strain and raw material can meet the formula requirement, the quality of a fermentation product is improved, the influence of impurities on strain metabolism can be reduced, the fermentation efficiency is improved, heat can be uniformly transferred, the temperature in the inner tank and the bottom end fermentation tank can be accurately controlled, and a suitable fermentation temperature environment is provided for multiple strains.

The invention relates to the technical field of resistance strain gauges, in particular to a high response frequency resistance strain gauge compatible with multiple strain directions, which comprises a substrate layer, a strain wire and an indication unit, a through hole is formed in the middle of the substrate layer; the indicating unit is arranged in the through hole; a plurality of upper pulleys are rotationally arranged on one side, in the width direction, of the base layer; the distances between the adjacent upper pulleys and lower pulleys in the length direction are the same; the strain wire is wound on the upper pulley and the lower pulley in sequence; the substrate layer is rotationally provided with an upper tensioning wheel and a lower tensioning wheel; one end of the strain wire is connected with the upper tensioning wheel; and the other end of the strain wire is connected with the lower tensioning wheel. The length of the strain wire between the upper pulley and the adjacent lower pulley can be detected, if the length is larger than the initial length L, it is judged that the to-be-detected object has strain in the width direction, and if the length is smaller than the initial length L, it is judged that the to-be-detected object has strain in the length direction, two sets of strain wires do not need to be arranged, and cost is effectively saved.

The present invention proposes a method for obtaining a staged damage shear constitutive model that accounts for the rate effect of composite materials. The method first conducts dynamic shear mechanical property tests of composite materials at multiple strain rates, recording the shear strain rate and shear stress-strain data for each test. A staged damage shear constitutive model with unknown parameters is proposed based on the characteristics of staged damage to the fiber and matrix within the composite material. A particle swarm optimization algorithm is used to obtain the parameter values ​​of the staged damage shear constitutive model corresponding to each test data point at the corresponding strain rate. A polynomial fitting method is used to obtain a functional relationship between the parameter values ​​of the staged damage shear constitutive model and the strain rate. The functional relationship between the parameter values ​​of the staged damage shear constitutive model and the strain rate is then substituted into the staged damage shear constitutive model with unknown parameters to obtain a staged damage shear constitutive model that accounts for the rate effect of the composite material. The present invention can characterize the shear constitutive relationship of composite materials under dynamic loads.

The invention discloses a high-position multi-strain random grafting crown-changing method for big star anise trees, which quickly utilizes the advantages of the big trees: relying on the original developed root system and nutrient supply capability of the big star anise trees, high-position grafted scions can quickly grow, and compared with re-planting seedlings, the method can greatly shorten the fruiting period and realize'fast fruiting and early high yield 'of old trees; by means of mixed grafting of multiple strains, the risk that a single strain is affected by specific diseases and insect pests and extreme climate can be reduced, the overall stress resistance of plants is improved, the yield stability is guaranteed, and the strain structure is optimized; and by virtue of the characteristics (such as more results and high quality) of other strains, scientific optimization of a single strain structure is realized, and comprehensive economic benefits are improved.

The application provides a wind turbine fault-tolerant operation method when a blade root load measurement fails, which first generates a blade load replacement algorithm based on a blade load theoretical algorithm, and specifically adjusts a constant value in the blade load theoretical algorithm, wherein the constant value at least includes a proportional coefficient corresponding to an acquisition result of each data acquisition point when participating in operation, that is, when a single strain sensor fails, only the proportional coefficient corresponding to the strain sensor needs to be set to 0, so that the strain measurement value of the strain sensor is not used, and meanwhile, the proportional coefficients of the strain measurement values of the remaining three strain sensors are automatically adjusted to complete the blade load calculation, which can effectively avoid the problem of wind turbine shutdown caused by the abnormality of a single strain sensor, fully utilizes the redundant design of the multiple strain sensors, improves the reliability of the unit, and guarantees the normal operation of the wind turbine. Meanwhile, the application also provides a fault-tolerant operation system applying the wind turbine fault-tolerant operation method when the blade root load measurement fails.

The invention provides a method and equipment for generating a strain-energy curve of a material. The method is applied to material science and engineering fields. The method comprises the following steps: acquiring an original structure of a to-be-processed material; performing mechanical matrix solution on the original structure to obtain Poisson ratios in multiple strain directions; determining a balance structure in each strain direction, continuously increasing the strain by taking the original structure as a starting point for each strain direction, determining a unit cell parameter according to a Poisson's ratio and a current strain value, and determining a balance structure under the current strain until the current strain reaches a preset maximum strain corresponding to the strain direction; obtaining a balance structure under the preset maximum strain in the current strain direction; and acquiring energy under each strain corresponding to each balance structure in each strain direction, and generating a strain-energy curve C1 of the to-be-processed material under the condition of increasing the strain. According to the method, the calculation power consumption is reduced, and the calculation result can be ensured to be a global minimum value without falling into a local minimum value.

The application relates to a foodborne microorganism detection method and system based on spectral technology. The method comprises the following steps: performing correlation analysis on the content sequence of each chemical substance in a plurality of blank matrix samples and the absorbance sequence of each wave band in the spectral data of the plurality of blank matrix samples to obtain an aliasing coefficient of each wave band; performing analysis on the content of each strain in a plurality of strain samples and the absorbance of each wave band in the corresponding strain spectral data to obtain an absorbance influence coefficient between any two strains under each wave band; determining a strain group representative coefficient of each wave band according to the number of the plurality of strains, the aliasing coefficient of each wave band and the absorbance influence coefficient of other strains on the first strain under the corresponding wave band; and screening a characteristic wave band from each wave band according to the strain group representative coefficient of each wave band and the absorbance sequence of the corresponding wave band in the spectral data of a plurality of target samples. The method simplifies the analysis difficulty of the coexistence of multiple strain groups in a complex matrix, and improves the generalization ability and practical application value of the model.

The invention discloses a multi-strain-rate and multi-waveform impact load integrated excitation device and a test method, and belongs to the technical field of civil engineering mechanical tests, the multi-strain-rate and multi-waveform impact load integrated excitation device comprises a common hydraulic loading system, a drop hammer driving device loading system, a dynamic hydraulic loading system, a pneumatic driving device loading system and a blasting driving device loading system; the common hydraulic loading system comprises a common oil cylinder, and the common oil cylinder is used for outputting hydraulic static load; the drop hammer driving device loading system is used for generating a drop hammer impact dynamic load with a medium strain rate; the dynamic hydraulic loading system is used for outputting any waveform pulse dynamic load with medium and low strain rates; compared with the prior art, the device has the advantages that various forms of impact loads can be applied, static loads and dynamic loads can be applied to the same test model at the same time, then the mechanical response of deep engineering rock mass under the superposition action of high ground stress static loads and multi-form impact disturbances can be simulated more accurately, and the device has the advantages of being high in integration level, high in applicability and accurate and controllable.

This invention relates to the field of bridge testing and inspection technology, specifically disclosing a test method for damage identification of precast assembled beam bridges based on strain curves. The method includes: arranging multiple strain test points on the precast assembled beam bridge to be tested; applying a moving load and collecting strain data from the strain sections; extracting the strain data from each strain section and performing noise reduction processing on the strain data to obtain the strain influence lines for each strain section; determining the longitudinal damage location: determining the longitudinal damage location based on the changes in the intersection positions of strain influence lines at different spans. This invention uses strain influence lines at different spans of the longitudinal beams of the precast assembled beam bridge to correct errors caused by residual strain influence and reduce noise in the test data, reducing errors caused by equipment and environmental factors. Changes in the intersection points of the strain influence lines enable longitudinal damage identification of the precast assembled beam bridge. The test is short, has minimal impact on bridge traffic, reduces test costs, and improves economic efficiency.

The invention belongs to the technical field of biological denitrification of aquaculture sewage, and particularly relates to a multi-strain-ceramsite carrier compound for static denitrification purification of aquaculture sewage as well as a preparation method and application of the multi-strain-ceramsite carrier compound. The compound is composed of eight strains of cellulomonas flavigena, bacillus subtillis, bacillus licheniformis, leuconostoc mesenteroides, pediococcus pentosaceus and paracoccus denitrificans, and the eight strains are loaded on a ceramsite carrier through a special process. When the compound disclosed by the invention is used for treating aquaculture sewage, a layered bacterium feeding mode is adopted, and odor can be remarkably inhibited when water is efficiently purified. Practical application results show that after being treated by the compound, the removal rates of total nitrogen, ammonia nitrogen and COD (Chemical Oxygen Demand) in the breeding sewage respectively reach 89.2%, 91.2% and 82.2%. According to the invention, through synergistic interaction of multiple strains, process optimization and accurate regulation and control of spatial distribution, efficient static purification of aquaculture sewage is realized, and an economical and feasible technical path is provided for rural environmental pollution abatement.

The invention discloses a construction method of a high / low pyruvate xanthan gum production strain, and relates to the technical field of biology, the construction method comprises the following steps: S1, preparing an original strain Xanthomonas campestris (Xanthomonas campestris); s2, measuring a growth curve of the original strain, and determining a logarithmic growth phase; s3, performing mutagenesis on the original strain bacterial liquid in the logarithmic phase by adopting ARTP, wherein the mutagenesis time is 160-200s; s4, high-throughput primary screening: culturing the mutagenized strains to obtain fermentation liquor, measuring the content of pyruvic acid in the fermentation liquor, and screening out a plurality of strains with higher or lower pyruvic acid content; s5, re-screening: re-screening the strains screened out in the step S4, and screening out the strains which are stable in pyruvic acid content and have the xanthan gum yield equal to that of the original strains. According to the method, orientated mutagenesis is carried out on xanthomonas campestris through an ARTP mutagenesis technology, high-throughput primary screening and multiple rounds of secondary screening are combined, and a mutant strain with the pyruvic acid content remarkably deviating from that of an original strain can be efficiently screened out.

This application provides a method and apparatus for mining strain-specific fragments, relating to the field of bioinformatics technology. The method includes: comparing the gene similarity of a target strain with multiple strains of the same species, and identifying the strain with the highest gene similarity as a reference strain; performing collinear alignment of the genomes of the target strain and the reference strain to identify and extract genomically differential fragments present in the target strain but not in the reference strain; segmenting the genomically differential fragments into multiple candidate fragments; identifying candidate fragments that meet preset conditions as specific standard fragments; and merging multiple specific standard fragments that are adjacent or overlapping to generate a specific fragment for the target strain. The method and apparatus provided in this application enable efficient and accurate identification of nucleotide fragments that uniquely identify a specific target strain from massive amounts of genomic data, improving the accuracy, reliability, and efficiency of detecting specific target microorganisms.

The application discloses a method for strain-level classification of metagenomic data based on a pan-genome graph, which first captures the genomes of multiple strains of each species as a reference through the technology of the pan-genome graph, instead of using only the representative genome of each species as a reference, expands the strain diversity, and enhances the alignment accuracy of metagenomic sequencing reads and the estimation of composition at the strain level; then, by using the corresponding short read alignment tool Giraffe and the long read alignment tool GraphAligner in the alignment of NGS and TGS metagenomic data with the pan-genome graph, two types of data can be efficiently processed at the same time; finally, by further optimizing the path abundance of the species-level classification results based on the pan-genome graph, multi-species strain-level classification can be realized. The application can solve the technical problem that the strain-level classification capability is limited due to the strategy of a single reference genome in the prior art.

The invention particularly relates to a traditional Chinese medicine multi-strain synergistic fermentation device, and belongs to the technical field of traditional Chinese medicine ferment.The traditional Chinese medicine multi-strain synergistic fermentation device comprises a culture barrel body and a fermentation barrel body, the culture barrel body is fixedly installed at the top of the fermentation barrel body, and a plurality of culture cavities are evenly formed in the culture barrel body; a material pushing assembly used for pushing the traditional Chinese medicine matrix in the culture cavity to move is arranged on the culture barrel body, a slidable discharging plate is arranged at the bottom in the culture cavity, a stirring assembly is arranged in the fermentation barrel body, and a liquid outlet assembly is arranged at the bottom of the fermentation barrel body, so that fermentation liquid is guided out. According to the traditional Chinese medicine fermentation device, multiple strains can be independently cultured, the different strains and crushed traditional Chinese medicine matrixes are independently cultured, the multiple strains which are independently cultured can be conveniently guided into the fermentation barrel body for mixed fermentation, and through cooperation of the stirring assembly, the traditional Chinese medicine matrixes mixed with the different strains can be efficiently mixed; therefore, the fermentation efficiency is improved.

The invention provides a rapid wheat breeding method and system based on stripe rust resistance gene editing and digital twinborn control, and relates to the technical field of digital breeding. The method comprises the following steps: obtaining a genome section where Yr61 is located, positioning a resistance-related target nucleotide sequence, and constructing a guide RNA and a gene editing carrier to obtain a multi-type editing group; the method comprises the following steps: acquiring disease process and agronomic character data under the conditions of multiple strains and multiple environments, and constructing and calibrating a digital twinborn model which takes editing types, growth processes, disease development and environment variables as state variables; and implementing rapid generation addition and selection according to model output, and carrying out backflow iteration updating on new-generation data. According to the method, closed-loop optimization of gene editing, phenotype identification and breeding decision is realized, the resistance stability is improved, and the breeding process is remarkably accelerated.

This invention discloses a method for improving the processing quality of tobacco stems through the combined use of multiple strains, comprising the following steps: Bacillus subtilis CX-26, Priestella megaterium CX17 enzyme preparation, and Bacillus belyssus DF-08 enzyme preparation are prepared through steps such as strain activation, seed culture preparation, and large-scale cultivation to obtain three enzyme preparations. The CX-26 and CX17 enzyme preparations are mixed at a 2:1 mass ratio. During the stem soaking stage, the mixed enzyme preparation is added to the soaking aqueous solution, and the high-temperature environment during the stem steaming stage inactivates the mixed enzyme preparation. After the tobacco stems are added, the DF-08 enzyme preparation is applied to the stems, and the high-temperature environment during the stem drying stage inactivates the enzyme preparation. This application offers advantages such as low tobacco stem processing cost, short processing cycle, safety and reliability, and the ability to degrade various macromolecular components such as pectin, cellulose, protein, and starch during tobacco stem processing, effectively improving tobacco stem quality.

The application relates to the technical field of resistance strain gauges, and particularly relates to a high-response-frequency resistance strain gauge compatible with multiple strain directions, which comprises a base layer, a strain wire and an indicating unit; the middle part of the base layer is provided with a perforation; the indicating unit is arranged in the perforation; a plurality of upper pulleys are rotationally arranged on one side of the base layer along the width direction; the distance between adjacent upper pulleys and lower pulleys in the length direction is the same; the strain wire is sequentially arranged around the upper pulleys and the lower pulleys; the base layer is rotationally provided with an upper tension pulley and a lower tension pulley; one end of the strain wire is connected with the upper tension pulley; the other end of the strain wire is connected with the lower tension pulley. According to the length of the strain wire between the upper pulley and the adjacent lower pulley, if the length is greater than the initial length L, it is judged that the strain of the measured object occurs in the width direction; if the length is less than the initial length L, it is judged that the strain of the measured object occurs in the length direction. Two groups of strain wires are not needed, and the cost is effectively saved.

The invention belongs to the technical field of wastewater and sewage treatment, and relates to a preparation method and application of a multi-strain-ceramsite carrier compound for static denitrification purification of aquaculture sewage. The multi-strain-ceramsite carrier compound consists of rhodopseudomonas palustris, pediococcus pentosaceus, bacillus licheniformis, candida albicans, leuconostoc mesenteroides, cellulomonas flavigena and staphylococcus saprophyticus, and is prepared by loading the multi-strain-ceramsite carrier compound on a ceramsite carrier through a special process. When the compound is used for treating aquaculture sewage, strain layered feeding is realized through ceramsite carrier density difference, the aquaculture sewage can be efficiently treated, and odor generation can be remarkably inhibited. Application results show that the total nitrogen degradation rate, the ammonia nitrogen degradation rate and the COD degradation rate of the culture sewage treated by the method are 86.2%, 88.2% and 79.2% respectively. Through the synergistic effect of multiple strains, process innovation and precise spatial distribution control, efficient static purification of the breeding sewage is achieved, and an economical and feasible technical scheme is provided for rural pollution treatment.

The invention relates to preparation of a multi-strain-ceramsite carrier compound and application of the multi-strain-ceramsite carrier compound in denitrification purification of aquaculture wastewater, and belongs to the field of aquaculture wastewater treatment. The compound is prepared from various strains such as aspergillus niger, pseudomonas fluorescens, bacillus subtillis, bacillus licheniformis, rhodopseudomonas palustris, pediococcus pentosaceus, nitrosomonas, paracoccus denitrificans and cellulomonas flavigena, and the strains are fixed in a ceramsite carrier by adopting a specific process. In the aquaculture sewage treatment process, the compound is added in a layered manner, so that efficient denitrification and decontamination of sewage are realized, and generation of odor is effectively inhibited. Practical application data show that the removal rates of total nitrogen, ammonia nitrogen and COD (Chemical Oxygen Demand) in the sewage respectively reach 88.2%, 90.2% and 81.2% after the sewage is treated by the compound. By means of multi-strain collaboration, process optimization and spatial distribution regulation and control, efficient static treatment of aquaculture sewage is achieved, and an economical and feasible solution is provided for rural environment pollution control.