256 results about "Multiple strain" patented technology

The present invention provides probe arrays and methods of using the same for concurrent and discriminable detection of multiple strains of different species. In one aspect, the probe arrays of the present invention are nucleic acid arrays comprising (1) a first group of probes, each of which is specific to a different respective strain of a first species; and (2) a second group of probes, each of which is specific to a different respective strain of a second species. In many embodiments, the nucleic acid arrays of the present invention further include a third group of probes, each of which is specific to a different strain of a third species. In one example, a nucleic acid array of the present invention includes probes for sequences selected from SEQ ID NOs: 1 to 18,598, and can discriminably detect different strains of Streptococcus pyogenes, Streptococcus agalactiae and Staphylococcus epidermidis.

The invention discloses a denitrifying strain with nitrifying function, a strain-containing water body improver of multiple active microorganisms and a preparation method of the water body improver, belonging to the technical fields of aquaculture and water body improvement. The strain disclosed by the invention is conserved in the China center for type culture collection (CCTCC), the CCTCC NO isM2011443; and the water body improver of the active microorganisms which is based on the strain comprises multiple strains such as Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus cereus, Bacillus megaterium, Rhodopeudomonas palustris, Arthrobacter globiformis, Rhodotorula rubra and Lactobacillus plantarum. The strain is used to perform liquid fermentation and obtain fermentation liquor of which number of live bacteria is 0.5-3*10<10>cfu / mL, bacterial powder of which the number of live bacteria is 0.2-2*10<12>cfu / mL is further prepared; and the bacterial powder is used along with a diluent to prepare a liquid or powdery water body improver, wherein the effective live bacteria of the water body improver is 0.1-3*10<10>cfu / mL(or cfu / g). The water body improver has the function of reducing the contents of ammonia nitrogen, nitrites and nitrates in the culture water body; and the water body improver is suitable for different culture water bodies and has the function of improving the water quality.

Nucleic acid arrays and methods of using the same for concurrent or discriminable detection of different strains of a non-viral species. In many embodiments, the nucleic acid arrays of the present invention include probes that are specific to different respective strains of a non-viral species. In many other embodiments, the nucleic acid arrays of the present invention include probes that are common to two or more different strains of the non-viral species. In one embodiment, the non-viral species is Staphylococcus aureus, and the different Staphylococcus aureus strains include COL, N315, Mu50, EMRSA-16, MSSA-476, and 8325 strains. In another embodiment, a nucleic acid array of the present invention includes polynucleotide probes capable of hybridizing under stringent or nucleic acid array hybridization conditions to respective sequences selected from SEQ ID NOs: 1 to 7,852, or the complements thereof.

The invention discloses a method for preparing a cow feed additive containing traditional Chinese medicine active probiotics, comprising the following steps: respectively carrying out amplification culture of the strain number of a single bacterial specie on original strains of active probiotics for cows; mixing primary seed liquors of active probiotics for cows with same weight, inoculating the mixture in a seed tank medium to carry out multiple-strain mixed culture, then inoculating in a traditional Chinese medicine liquid medium to carry out a primary traditional Chinese medicine fermentation; and mixing the multiple-strain mixed traditional Chinese medicine fermentation liquor with isometric cob powder to obtain solid fermentation seeds, and then inoculating in a traditional Chinese medicine solid medium to carry out a secondary traditional Chinese medicine fermentation. According to the invention, the strain activity and colonization performance in the intestinal tracts of activeprobiotics in the cow feed additive are improved greatly, the prepared cow feed additive is rich in traditional Chinese medicine components, the active components with pharmacological effects in the traditional Chinese medicine components fully play the role, thus the performances of strengthening the body, treating diseases and promoting growth of the cow feed additive are improved, and furthermore completely displace antibiotics.

Multiple strains and species of Campylobacter were found to share a common glycan moitie which is present in a plurality of surface-exposed glycoproteins. This glycan has the formula: GalNAc-a1,4-GalNAc-a1,4-[Glc-β1,3]GalNAc-a1,4-GalNAc-a1,4-GalNAc-a1,3-Bac, wherein Bac is 2,4-diacetamido-2,4,6-trideoxy-D-glucopyranose. This glycan and immunologically active fragments of it have use as vaccines against campylobacter infection in humans and animals. As well, antibodies which specifically bind these compounds may be provided. Such antibodies and vaccines may be used to prevent or neutralize campylobacter infections in livestock thereby preventing this pathogen from entering the human food chain. The glycan may be linked to one or more amino acids to form a glycopeptide. As well, a method for determining the glycan structure of an intact glycoprotein consists of subjecting a sample to high resolution magic angle spinning nuclear magnetic resonance (HR-MAS-NMR) spectroscopy.

The invention discloses a method for producing high-salt diluted soybean sauce through cooperative fermentation of multiple strains. The method comprises the following steps: preparing a mold starter, processing a raw material, making the starter, making mash, fermenting, oil-pumping, blending, sterilizing and packaging. Aspergillus oryzae and aspergillus niger are compounded to make the starter during the preparation of the mold starter, and Japanese schizosaccharomyces and pediococcus pentosaceus are properly added in a soy sauce mash fermentation process. The method for producing the high-salt diluted soybean sauce through cooperative fermentation of multiple strains, provided by the invention, has the characteristics of high raw material protein utilization rate, and low cost; the produced soy sauce is thick in flavor, mellow in grease flavor, and sufficient in delicate flavor.

The invention relates to a preparing method for poly strains microbe leaven and its application in cow concentrated feed. It belongs to microbe ferment field of bioengineering. The invention adopts multiple microbe strains, and combines them in ratio to produce the leaven. Evenly mixing the raw material and straws, then adding to activated leaven, packing them to the container to ferment for obtaining the finished product. The feeding stuff manufactured by the leaven raises the milk output 0.5 to 1kg per day, prolongs the stage of lactation, inhibits the intestinal canal bacteria growth, increases the cow appetite, raises the feeding stuff utilization factor by 15%. The analysis to the feeding stuff shows that crude protein is increased by 20-25%, the raw fiber is decreased by 10-15%.

The invention discloses multi-strain combined fermentation red date wine and a brewing method thereof, which belongs to the technical field of brewing. The red date wine is produced by taking red date as raw materials through such processes as pretreatment, digestion, enzymolysis, sterilization, fermentation, aging and clarification. The adopted Candida, Kloeckera, Hanseniaspora and Pichia pastoris move at the early stage of fruit wine fermentation generally, so that a great deal of aromatic substances and special flavor components can be generated, the quality of fruit wine is improved, and positive impact is generated on the total flavour of the red date wine. Non-brewing yeast and brewing yeast are accessed at different fermentation stages, the species and content of the flavor substances in the date wine can be increased through the combined fermentation of multiple strains, thereby improving the mouthfeel of the date wine and the quality of the date wine; the date wine has unique style, rich wine body, sweet and pure bouquet, fresh mouthfeel and typicality.

The invention discloses a phosphorus and calcium type efficient and refined multiple-strain microbial organic fertilizer and a preparation method thereof, relating to the field of agricultural fertilizer. The microbial organic fertilizer is prepared by the following components in parts by weight: 44-54 parts of organic matter wastes, 10-15 parts of potassium chloride, 10-15 parts of humic acid, 0.4-0.6 part of intermediate trace elements, 0.4-0.6 part of microorganism, 5-10 parts of urea, 5-15 parts of superphosphate and 5-15 parts of diammonium phosphate (DAP) through the steps of mixing and pelletizing. The microbial organic fertilizer has the characteristics of good multi-bacteria symbiotic environment, stronger bioactivity, high fertilizer efficiency, full range of organic matters, effective micropopulation and chemical nutrients and controlled release effects. When in use, the phosphorus and calcium type efficient and refined multiple-strain microbial organic fertilizer in the invention can enhance the soil fertility, improve the fertilizer utilization rate, improve agricultural product quality, enhance plant diseases and insect pest resistance and drought resistance, inhibit crops from absorbing harmful substances such as heavy metals, pesticide and the like, purify and restore soil and the like, and is suitable for various crops and soils.

The present application describes methods for detecting influenza A and / or influenza B and / or distinguishing between pathogenic and seasonal influenza A subtypes. Many of these preferred formats employ pan-specific antibodies (i.e., that react with all or at least multiple strains within an influenza type) to detect presence of influenza A and / or influenza B and PDZ domains in combination with panspecific antibodies to influenza A to distinguish pathogenic and seasonal influenza A subtypes.

The invention relates to the microbiological engineering technology. Crop byproducts, such as agricultural straws, hulls and cakes and the like are utilized to produce high protein feed and animal promoters by multiple strains to lead wastes to be effectively utilized; waste materials are changed into valuable things, so that raw materials are inexhaustible, thus improving input-output ratio of feed with significant economic and social benefits. As containing a great number of active enzymes and growth promoting factors, the technology improves the function of animal metabolism, and leads theactive enzymes and growth promoting factors to generate mutual synergism and comprehensive balance to animal organisms, thus ensuring that nutrient substances can be rapidly degraded in animal intestinal canals, promoting animal to absorb and transform nutritious ingredients in the feed, and achieving the purposes of promoting animal growth and saving feed. The technology is mainly applied in animal feed promoter, such as pigs, chickens, cows and sheep and the like and biological high protein feed. The invention has easily mastered production method and process, less investment, low cost and is suitable for investing and building factories for individuals and medium-sized and small enterprises.

The invention provides a multifunctional titanium implant based on polydopamine modification and a preparation method of the multifunctional titanium implant. The multifunctional titanium implant is prepared as follows: a silane coupling agent is added to the surface of pre-activated titanium metal and subjected to an auto-polymerization reaction with polydopamine, functional groups are grafted onthe surface of a polydopamine coating obtained through the auto-polymerization reaction, and finally, the multifunctional titanium implant is obtained, wherein the bonding strength between the polydopamine coating and the surface of the titanium metal is 4.5-6.5 MPa. The multifunctional titanium implant adopts a porous surface structure, has good cell biocompatibility, has an efficient antibacterial effect on peri-implantitis specific bacteria and particularly has a significant synergistic antibacterial effect on multiple strains. The preparation method of the multifunctional titanium implanthas the characteristics of being simple in process and suitable for promotion.

The invention relates to a production method of a sweet osmanthus tree vase, comprising the following steps: S1, branch selection and cottage; S2, branches transplanting: selecting flourishing and strong sweet osmanthus tree seedlings, and then carrying out determination and planting on the seeldings; S3, seedling cultivation; S4, knitting and modeling: selecting seedlings with the same height and size from the cultured sweet osmanthus tree seedlings, ensuring multiple strains of the sweet osmanthus tree seedlings to be distributed and arranged according to circumference with a certain number so as to form a cluster of the sweet osmanthus trees, managing and cultivating the cluster of the sweet osmanthus trees to let the sweet osmanthus tree seedlings grow and survive; and after the sweet osmanthus tree grows and survives, knitting and modeling the setting mould of the sweet osmanthus tree to enable the branches of a plurality of the sweet osmanthus tree seedlings to be curved and enveloped into a vase structure, and carrying out manual treatment on the trunk crossing section of the sweet osmanthus tree to ensure the natural growth and coalescence of the trunks so as to be connected together. The invention has the beneficial effects of enabling the joint of the sweet osmanthus tree to naturally heal and grow into a whole, thus the sweet osmanthus tree vase formed in the way is firmer.

The invention relates to a multi-channel AC / DC excitation signal measuring system for a strain force sensor. A digital signal processor (DSP) is taken as the core, an AC / DC excitation mode is adoptedto select an excitation scheme with adjustable AC excitation frequency, a digital control scheme with automatic optimization of excitation frequency and modulation frequency band is adopted, a digitallow-pass filter, amplitude correction and digital signal amplification processing flow is adopted for a DC excitation mode, and a digital band-pass filter, digital signal demodulation, vector correction and digital signal amplification processing flow is adopted for an AC excitation mode, so that multi-channel and high precision signal measurement of the strain force sensor under different interference environments is realized. The system includes a hardware system and a software signal processing method. Through adoption of the multi-channel AC / DC excitation signal measuring system, the selection of the excitation mode and the signal processing flow can be automatically optimized, and the influences of excitation signal fluctuation and line parasitic parameters can be corrected, therebyrealizing high-precision measurement of single or multiple strain force sensors with up to eight measuring bridges.

The invention discloses a composite leavening agent combining xylanase with multiple strains and a method for fermenting a straw feed. The leavening agent consists of the following components in part by weight: 4 parts of xylanase, 2 parts of lactobacillus acidophilus, 2 parts of propionibacterium, 2 parts of saccharomyces cerevisiae, 3 parts of bacillus subtilis, 1 part of lactobacillus plantarum and 1 part of bacillus coagulans, wherein the xylanase can quickly degrade cell walls of straw plants and release nutrient substances; and under the synergistic effects of a plurality of microorganisms, fermentation speed is increased, fermentation period is shortened, fermentation activity is enhanced, protein conversion rate is increased and fermentation cost is lowered. The prepared fermented straw feed has low cellulose content, high protein content, high viable count, long retention period, soft and wet feel, strong acid incense, high palatability and high nutritional value. The problem of low resource utilization ratio of crops such as straws and the like is solved; simultaneously, environmental pollution is reduced; and the leavening agent and the method have high economic benefit and social benefit.

The invention discloses a small six-axis force and torque sensor, which comprises a cylindrical shell, a base arranged on the inner wall of the cylindrical shell, eight first elastic beams with the same size and the same structure connected with the base, a central boss arranged in the center of the cylindrical shell, and four second elastic beams with the same size and the same structure connected with the central boss and the first elastic beams, wherein the four second elastic beams are arranged in a cross shape; and multiple strain gauges are attached to the eight first elastic beams and the four second elastic beams respectively to form full bridge circuits respectively. The small six-axis force and torque sensor is simple in structure, delicate in size, can realize structure self decoupling in six direction, and is suitable for matched use in the current industrial system; and as the cylindrical shell, the base, the central boss, the first elastic beams and the second elastic beams are all integral components, assembly is not needed, and the small six-axis force and torque sensor has the advantages of high strength, large stiffness, high nature frequency and ideal linearity, repeatability and hysteresis.

The invention belongs to the field of microorganisms and discloses a biological agent for treating ammonia-nitrogen-containing wastewater. The biological agent comprises rhodococcus ruber, micrococcus luteus, enterococcus faecalis, acinetobacter baumannii, arthrobacter crystallopoietes, thiobacillus denitrificans and pseudomonas fluorescens. The biological agent comprises multiple strains, has the advantages of reasonable compatibility and strong synergic effect and can be used for effectively removing ammonia nitrogen, sulfide, aniline substances and industrial COD in wastewater.

Epicatechins, Epicatechin Oligomers, or Thiolated Epicatechins are applied (A) directly to a genital wart in the form of a cream, ointment, paste or solution, (B) directly to the genital wart wherein such cream, ointment, paste or solution contains as an additional active ingredient a skin permeabilizing agent, (C) following electrosurgical resection or removal of the genital wart in such form of a cream, ointment, paste or solution, (D) following chemical resection or extirpation of the genital wart in such form, (E) following surgical resection or removal of the genital wart in such form, wherein said Epicatechins, Epicatechin Oligomers, or Thiolated Epicatechins both provide antiviral activity against multiple strains of human papilloma virus (HPV) and promote healing following resection polymers contained in a vehicle. Disclosed are the compositions, therapeutical kits containing such composition, methods of treatment using such composition, and methods of enhancing the stability of such composition.

Compositions and methods useful for reducing or eliminating the presence of pathogens in meat and meat products are disclosed. Administration of one or more lactic acid producing microorganisms to a live animal, to a carcass, to meat, to meat products, or in animal feed results in significant reductions in the amount of pathogens potentially harmful to humans when ingested. Synergistic effects can be achieved with the administration of multiple strains of microorganisms.

The invention discloses a rotor axial force measurement device and measurement method which are used for measuring axial force of a rotor mounted on an elastic support provided with a bearing outer ring. The elastic support comprises a mounting edge and a free end opposite to the mounting edge, the bearing outer ring is arranged at the free end, and the space between the mounting edge and the free end is partially hollowed-out, so that a plurality of elastic strips distributed in the circumferential direction at intervals are formed. The measurement device comprises multiple strain gauges symmetrically arranged on the elastic strips along the center axis of the elastic support, the stain gauges are connected in series, led out through a signal line and then connected to a strain monitor, and the strain monitor is used for acquiring a measurement result of the axle force of the rotor arranged on the elastic support according to strain output values detected by the multiple strain gauges. The strain gauges are arranged on the elastic strips of the elastic support and connected to form a whole bridge for induction of the axial force of the rotor, no extra appended structure is required, an original axial force measurement system is simplified, and the problem that axial force of the rotor on the elastic support provided with the bearing outer ring cannot be measured is solved.

The invention provides preparation of a total-nutrient efficient biological compound fertilizer and a special microbial strain. The fertilizer is a microbiological multiple-strain compound fertilizer which is formed by carrying out fermenting, granulating and coating on oil shale ground slag, chicken manure, multiple strains and inorganic nutrients and is suitable for different soils and the growth of crops. The biological compound fertilizer can obviously improve the content of corresponding quick-acting nutrients in soil, and can increase the yield of various crops by 10-30%. The invention has simple and convenient process, easy operation, wide raw material sources and low manufacture cost.

The invention provides a method for performing mating type gene identification on a basidiospore monocaryon strain which is separated from different hypsizigus marmoreus strains. In the identifying process, in particular application of a flushing reaction, a palisade reaction and transplanting, the method can primarily identify the mating type gene of the hypsizigus marmoreus intuitively and quickly without other instruments and equipment. When the method is applied in cross breeding, the mating type gene of basidiospore monocaryon parent and progeny strains can be identified conveniently so as to improve the purposiveness and the effectiveness of the cross breeding process. The adopted method can shorten the time for identifying the mating type gene for 5 to 10 days, reduce the number of comparing-screening strains by 75 percent theoretically in cross breeding, improve the fecundity, and is more convenient and efficient particularly in application of multiple cross breeding of multiple strains. Then, the method selects the compatible basidiospore monocaryon strain having clamp connection according to the identification result to carry out biparental crossing, and screens hybrid strains according to growth speed, growth potential and economical character index, thereby obtaining stable, high yield and high quality hybrid strains.

The present invention relates to a production method for soy sauce made from bean dregs through multiple strains. The soy sauce uses bean dregs, wheat bran and the like as raw materials, and is made through the fermentation by a plurality of microorganisms. The fermentation starter essence used in the present invention comprises Aspergillus oryzae strain CICC2339 (Huniang 3.042) as a main strain, Aspergillus niger and a plurality of other strains as auxiliary strains, and is especially added with active aroma-producing yeast, torularhodin and the like. The bean dregs in the raw materials have a high nutritional value and similar composition with tofu, and contain rich proteins, sugar, Ca, P, Fe and other minerals. The finished soy sauce product prepared from the bean dregs meets the requirements for all indexes. Therefore, the bean dregs can be used to replace soybean meal to produce the soy sauce. The soy sauce produced in the present invention has a bronzing color, thick sauce flavor, a delicious taste and moderate salinity. The quality of the soy sauce is much better than that of chemical soy sauce, blended soy sauce or animal hair soy sauce and the like on the market. The production method of the soy sauce comprises steps of raw material processing, starter propagation, fermentation, oil spraying, sterilization by heating, clarification by sedimentation, finished product preparation and the like.

The present invention features an array capable of monitoring gene expression patterns of multiple strains of Streptococcus pneumoniae including a substrate having a plurality of addresses, each of which has a probe disposed thereon.

According to a nine-crop nine-step making technology, glutinous sorghum, wheat, tartary buckwheat, agriophyllum squarrosum, rice, sticky rice, black rice, corn and mung bean are reasonably matched, eight times of feeding, nine times of stacking and nine times of baijiu flowing are conducted in the procedures of crop wetting, groove matching, grain groove steaming, baijiu flowing, stacking, cellar entering, cellar sealing, fermenting and storing in autumn, winter, spring and summer, suitable technological parameters are formulated according to the temperature differences of the different seasons, and therefore the advantages of the multiple raw materials, multiple yeast seeds and multiple strains are utilized to the maximum extent and the best fermentation result is obtained. According to the nine-crop nine-step making technology, unique features are shown in all the technical links, and the technology has the advantages that the fermentation period and the storage period are long. Compared with the prior art, the technology has the advantages that nine-grain aroma baijiu is created, the blank that no autonomous aroma baijiu exists in northwest is filled, the newest requirements for baijiu styles of modern people are met, the rate of baijiu making is up to 44.9% or above, the production cost is reduced, and the baijiu style has specific characteristics. The making method plays an important role in scientific development of baijiu.

The invention discloses a method for preparing a grass carp feed additive containing traditional Chinese medicine active probiotics, comprising the following steps: respectively carrying out amplification culture of the strain number of a single bacterial specie on original strains of active probiotics for grass carps; mixing primary seed liquors of active probiotics for grass carps with same weight, inoculating the mixture in a seed tank medium to carry out multiple-strain mixed culture, then inoculating in a traditional Chinese medicine liquid medium to carry out a primary traditional Chinese medicine fermentation; and mixing the multiple-strain mixed traditional Chinese medicine fermentation liquor with isometric cob powder to obtain solid fermentation seeds, and then inoculating in a traditional Chinese medicine solid medium to carry out a secondary traditional Chinese medicine fermentation. According to the invention, the strain activity and colonization performance in the intestinal tracts of active probiotics in the grass carp feed additive are improved greatly, the prepared grass carp feed additive is rich in traditional Chinese medicine components, the active components with pharmacological effects in the traditional Chinese medicine components fully play the role, thus the performances of strengthening the body, treating diseases and promoting growth of the grass carp feed additive are improved, and the grass carp feed additive can effectively treat and prevent four diseases of grass carps, and furthermore completely displace antibiotics.