256 results about "Multiple strain" patented technology

The present invention provides probe arrays and methods of using the same for concurrent and discriminable detection of multiple strains of different species. In one aspect, the probe arrays of the present invention are nucleic acid arrays comprising (1) a first group of probes, each of which is specific to a different respective strain of a first species; and (2) a second group of probes, each of which is specific to a different respective strain of a second species. In many embodiments, the nucleic acid arrays of the present invention further include a third group of probes, each of which is specific to a different strain of a third species. In one example, a nucleic acid array of the present invention includes probes for sequences selected from SEQ ID NOs: 1 to 18,598, and can discriminably detect different strains of Streptococcus pyogenes, Streptococcus agalactiae and Staphylococcus epidermidis.

The invention discloses a denitrifying strain with nitrifying function, a strain-containing water body improver of multiple active microorganisms and a preparation method of the water body improver, belonging to the technical fields of aquaculture and water body improvement. The strain disclosed by the invention is conserved in the China center for type culture collection (CCTCC), the CCTCC NO isM2011443; and the water body improver of the active microorganisms which is based on the strain comprises multiple strains such as Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus cereus, Bacillus megaterium, Rhodopeudomonas palustris, Arthrobacter globiformis, Rhodotorula rubra and Lactobacillus plantarum. The strain is used to perform liquid fermentation and obtain fermentation liquor of which number of live bacteria is 0.5-3*10<10>cfu/mL, bacterial powder of which the number of live bacteria is 0.2-2*10<12>cfu/mL is further prepared; and the bacterial powder is used along with a diluent to prepare a liquid or powdery water body improver, wherein the effective live bacteria of the water body improver is 0.1-3*10<10>cfu/mL(or cfu/g). The water body improver has the function of reducing the contents of ammonia nitrogen, nitrites and nitrates in the culture water body; and the water body improver is suitable for different culture water bodies and has the function of improving the water quality.

Nucleic acid arrays and methods of using the same for concurrent or discriminable detection of different strains of a non-viral species. In many embodiments, the nucleic acid arrays of the present invention include probes that are specific to different respective strains of a non-viral species. In many other embodiments, the nucleic acid arrays of the present invention include probes that are common to two or more different strains of the non-viral species. In one embodiment, the non-viral species is Staphylococcus aureus, and the different Staphylococcus aureus strains include COL, N315, Mu50, EMRSA-16, MSSA-476, and 8325 strains. In another embodiment, a nucleic acid array of the present invention includes polynucleotide probes capable of hybridizing under stringent or nucleic acid array hybridization conditions to respective sequences selected from SEQ ID NOs: 1 to 7,852, or the complements thereof.

The invention discloses a method for preparing a cow feed additive containing traditional Chinese medicine active probiotics, comprising the following steps: respectively carrying out amplification culture of the strain number of a single bacterial specie on original strains of active probiotics for cows; mixing primary seed liquors of active probiotics for cows with same weight, inoculating the mixture in a seed tank medium to carry out multiple-strain mixed culture, then inoculating in a traditional Chinese medicine liquid medium to carry out a primary traditional Chinese medicine fermentation; and mixing the multiple-strain mixed traditional Chinese medicine fermentation liquor with isometric cob powder to obtain solid fermentation seeds, and then inoculating in a traditional Chinese medicine solid medium to carry out a secondary traditional Chinese medicine fermentation. According to the invention, the strain activity and colonization performance in the intestinal tracts of activeprobiotics in the cow feed additive are improved greatly, the prepared cow feed additive is rich in traditional Chinese medicine components, the active components with pharmacological effects in the traditional Chinese medicine components fully play the role, thus the performances of strengthening the body, treating diseases and promoting growth of the cow feed additive are improved, and furthermore completely displace antibiotics.

Multiple strains and species of Campylobacter were found to share a common glycan moitie which is present in a plurality of surface-exposed glycoproteins. This glycan has the formula: GalNAc-a1,4-GalNAc-a1,4-[Glc-β1,3]GalNAc-a1,4-GalNAc-a1,4-GalNAc-a1,3-Bac, wherein Bac is 2,4-diacetamido-2,4,6-trideoxy-D-glucopyranose. This glycan and immunologically active fragments of it have use as vaccines against campylobacter infection in humans and animals. As well, antibodies which specifically bind these compounds may be provided. Such antibodies and vaccines may be used to prevent or neutralize campylobacter infections in livestock thereby preventing this pathogen from entering the human food chain. The glycan may be linked to one or more amino acids to form a glycopeptide. As well, a method for determining the glycan structure of an intact glycoprotein consists of subjecting a sample to high resolution magic angle spinning nuclear magnetic resonance (HR-MAS-NMR) spectroscopy.

A specimen for measuring a material under multiple strains and strain rates. The specimen including a body having first and second ends and a gage region disposed between the first and second ends, wherein the body has a central, longitudinal axis passing through the first and second ends. The gage region includes a first gage section and a second gage section, wherein the first gage section defines a first cross-sectional area that is defined by a first plane that extends through the first gage section and is perpendicular to the central, longitudinal axis. The second gage section defines a second cross-sectional area that is defined by a second plane that extends through the second gage section and is perpendicular to the central, longitudinal axis and wherein the first cross-sectional area is different in size than the second cross-sectional area.

The invention relates to a preparing method for poly strains microbe leaven and its application in cow concentrated feed. It belongs to microbe ferment field of bioengineering. The invention adopts multiple microbe strains, and combines them in ratio to produce the leaven. Evenly mixing the raw material and straws, then adding to activated leaven, packing them to the container to ferment for obtaining the finished product. The feeding stuff manufactured by the leaven raises the milk output 0.5 to 1kg per day, prolongs the stage of lactation, inhibits the intestinal canal bacteria growth, increases the cow appetite, raises the feeding stuff utilization factor by 15%. The analysis to the feeding stuff shows that crude protein is increased by 20-25%, the raw fiber is decreased by 10-15%.

The present invention relates to methods, polynucleotides, and polypeptides encoding immunogenic HIV polypeptides derived from different strains within an HIV subtype and/or immunogenic HIV polypeptides from different subtypes. Uses of the polynucleotides and polypeptides in combination approaches for generating immune responses are described. The combination approaches described herein have been shown to induce broad and potent neutralizing activity against diverse HIV strains from multiple strains within a given subtype and against diverse subtypes. Formulations of compositions for generating immune responses and methods of use for such compositions are also disclosed.

The present application describes methods for detecting influenza A and/or influenza B and/or distinguishing between pathogenic and seasonal influenza A subtypes. Many of these preferred formats employ pan-specific antibodies (i.e., that react with all or at least multiple strains within an influenza type) to detect presence of influenza A and/or influenza B and PDZ domains in combination with panspecific antibodies to influenza A to distinguish pathogenic and seasonal influenza A subtypes.

The invention relates to a production method of a sweet osmanthus tree vase, comprising the following steps: S1, branch selection and cottage; S2, branches transplanting: selecting flourishing and strong sweet osmanthus tree seedlings, and then carrying out determination and planting on the seeldings; S3, seedling cultivation; S4, knitting and modeling: selecting seedlings with the same height and size from the cultured sweet osmanthus tree seedlings, ensuring multiple strains of the sweet osmanthus tree seedlings to be distributed and arranged according to circumference with a certain number so as to form a cluster of the sweet osmanthus trees, managing and cultivating the cluster of the sweet osmanthus trees to let the sweet osmanthus tree seedlings grow and survive; and after the sweet osmanthus tree grows and survives, knitting and modeling the setting mould of the sweet osmanthus tree to enable the branches of a plurality of the sweet osmanthus tree seedlings to be curved and enveloped into a vase structure, and carrying out manual treatment on the trunk crossing section of the sweet osmanthus tree to ensure the natural growth and coalescence of the trunks so as to be connected together. The invention has the beneficial effects of enabling the joint of the sweet osmanthus tree to naturally heal and grow into a whole, thus the sweet osmanthus tree vase formed in the way is firmer.

The invention relates to a multi-channel AC/DC excitation signal measuring system for a strain force sensor. A digital signal processor (DSP) is taken as the core, an AC/DC excitation mode is adoptedto select an excitation scheme with adjustable AC excitation frequency, a digital control scheme with automatic optimization of excitation frequency and modulation frequency band is adopted, a digitallow-pass filter, amplitude correction and digital signal amplification processing flow is adopted for a DC excitation mode, and a digital band-pass filter, digital signal demodulation, vector correction and digital signal amplification processing flow is adopted for an AC excitation mode, so that multi-channel and high precision signal measurement of the strain force sensor under different interference environments is realized. The system includes a hardware system and a software signal processing method. Through adoption of the multi-channel AC/DC excitation signal measuring system, the selection of the excitation mode and the signal processing flow can be automatically optimized, and the influences of excitation signal fluctuation and line parasitic parameters can be corrected, therebyrealizing high-precision measurement of single or multiple strain force sensors with up to eight measuring bridges.

The invention discloses a composite leavening agent combining xylanase with multiple strains and a method for fermenting a straw feed. The leavening agent consists of the following components in part by weight: 4 parts of xylanase, 2 parts of lactobacillus acidophilus, 2 parts of propionibacterium, 2 parts of saccharomyces cerevisiae, 3 parts of bacillus subtilis, 1 part of lactobacillus plantarum and 1 part of bacillus coagulans, wherein the xylanase can quickly degrade cell walls of straw plants and release nutrient substances; and under the synergistic effects of a plurality of microorganisms, fermentation speed is increased, fermentation period is shortened, fermentation activity is enhanced, protein conversion rate is increased and fermentation cost is lowered. The prepared fermented straw feed has low cellulose content, high protein content, high viable count, long retention period, soft and wet feel, strong acid incense, high palatability and high nutritional value. The problem of low resource utilization ratio of crops such as straws and the like is solved; simultaneously, environmental pollution is reduced; and the leavening agent and the method have high economic benefit and social benefit.

The invention discloses a small six-axis force and torque sensor, which comprises a cylindrical shell, a base arranged on the inner wall of the cylindrical shell, eight first elastic beams with the same size and the same structure connected with the base, a central boss arranged in the center of the cylindrical shell, and four second elastic beams with the same size and the same structure connected with the central boss and the first elastic beams, wherein the four second elastic beams are arranged in a cross shape; and multiple strain gauges are attached to the eight first elastic beams and the four second elastic beams respectively to form full bridge circuits respectively. The small six-axis force and torque sensor is simple in structure, delicate in size, can realize structure self decoupling in six direction, and is suitable for matched use in the current industrial system; and as the cylindrical shell, the base, the central boss, the first elastic beams and the second elastic beams are all integral components, assembly is not needed, and the small six-axis force and torque sensor has the advantages of high strength, large stiffness, high nature frequency and ideal linearity, repeatability and hysteresis.

The invention belongs to the field of microorganisms and discloses a biological agent for treating ammonia-nitrogen-containing wastewater. The biological agent comprises rhodococcus ruber, micrococcus luteus, enterococcus faecalis, acinetobacter baumannii, arthrobacter crystallopoietes, thiobacillus denitrificans and pseudomonas fluorescens. The biological agent comprises multiple strains, has the advantages of reasonable compatibility and strong synergic effect and can be used for effectively removing ammonia nitrogen, sulfide, aniline substances and industrial COD in wastewater.

Epicatechins, Epicatechin Oligomers, or Thiolated Epicatechins are applied (A) directly to a genital wart in the form of a cream, ointment, paste or solution, (B) directly to the genital wart wherein such cream, ointment, paste or solution contains as an additional active ingredient a skin permeabilizing agent, (C) following electrosurgical resection or removal of the genital wart in such form of a cream, ointment, paste or solution, (D) following chemical resection or extirpation of the genital wart in such form, (E) following surgical resection or removal of the genital wart in such form, wherein said Epicatechins, Epicatechin Oligomers, or Thiolated Epicatechins both provide antiviral activity against multiple strains of human papilloma virus (HPV) and promote healing following resection polymers contained in a vehicle. Disclosed are the compositions, therapeutical kits containing such composition, methods of treatment using such composition, and methods of enhancing the stability of such composition.

The invention discloses a rotor axial force measurement device and measurement method which are used for measuring axial force of a rotor mounted on an elastic support provided with a bearing outer ring. The elastic support comprises a mounting edge and a free end opposite to the mounting edge, the bearing outer ring is arranged at the free end, and the space between the mounting edge and the free end is partially hollowed-out, so that a plurality of elastic strips distributed in the circumferential direction at intervals are formed. The measurement device comprises multiple strain gauges symmetrically arranged on the elastic strips along the center axis of the elastic support, the stain gauges are connected in series, led out through a signal line and then connected to a strain monitor, and the strain monitor is used for acquiring a measurement result of the axle force of the rotor arranged on the elastic support according to strain output values detected by the multiple strain gauges. The strain gauges are arranged on the elastic strips of the elastic support and connected to form a whole bridge for induction of the axial force of the rotor, no extra appended structure is required, an original axial force measurement system is simplified, and the problem that axial force of the rotor on the elastic support provided with the bearing outer ring cannot be measured is solved.

The invention provides preparation of a total-nutrient efficient biological compound fertilizer and a special microbial strain. The fertilizer is a microbiological multiple-strain compound fertilizer which is formed by carrying out fermenting, granulating and coating on oil shale ground slag, chicken manure, multiple strains and inorganic nutrients and is suitable for different soils and the growth of crops. The biological compound fertilizer can obviously improve the content of corresponding quick-acting nutrients in soil, and can increase the yield of various crops by 10-30%. The invention has simple and convenient process, easy operation, wide raw material sources and low manufacture cost.

The present invention relates to a production method for soy sauce made from bean dregs through multiple strains. The soy sauce uses bean dregs, wheat bran and the like as raw materials, and is made through the fermentation by a plurality of microorganisms. The fermentation starter essence used in the present invention comprises Aspergillus oryzae strain CICC2339 (Huniang 3.042) as a main strain, Aspergillus niger and a plurality of other strains as auxiliary strains, and is especially added with active aroma-producing yeast, torularhodin and the like. The bean dregs in the raw materials have a high nutritional value and similar composition with tofu, and contain rich proteins, sugar, Ca, P, Fe and other minerals. The finished soy sauce product prepared from the bean dregs meets the requirements for all indexes. Therefore, the bean dregs can be used to replace soybean meal to produce the soy sauce. The soy sauce produced in the present invention has a bronzing color, thick sauce flavor, a delicious taste and moderate salinity. The quality of the soy sauce is much better than that of chemical soy sauce, blended soy sauce or animal hair soy sauce and the like on the market. The production method of the soy sauce comprises steps of raw material processing, starter propagation, fermentation, oil spraying, sterilization by heating, clarification by sedimentation, finished product preparation and the like.

The present invention features an array capable of monitoring gene expression patterns of multiple strains of Streptococcus pneumoniae including a substrate having a plurality of addresses, each of which has a probe disposed thereon.

According to a nine-crop nine-step making technology, glutinous sorghum, wheat, tartary buckwheat, agriophyllum squarrosum, rice, sticky rice, black rice, corn and mung bean are reasonably matched, eight times of feeding, nine times of stacking and nine times of baijiu flowing are conducted in the procedures of crop wetting, groove matching, grain groove steaming, baijiu flowing, stacking, cellar entering, cellar sealing, fermenting and storing in autumn, winter, spring and summer, suitable technological parameters are formulated according to the temperature differences of the different seasons, and therefore the advantages of the multiple raw materials, multiple yeast seeds and multiple strains are utilized to the maximum extent and the best fermentation result is obtained. According to the nine-crop nine-step making technology, unique features are shown in all the technical links, and the technology has the advantages that the fermentation period and the storage period are long. Compared with the prior art, the technology has the advantages that nine-grain aroma baijiu is created, the blank that no autonomous aroma baijiu exists in northwest is filled, the newest requirements for baijiu styles of modern people are met, the rate of baijiu making is up to 44.9% or above, the production cost is reduced, and the baijiu style has specific characteristics. The making method plays an important role in scientific development of baijiu.

The invention discloses a method for preparing a grass carp feed additive containing traditional Chinese medicine active probiotics, comprising the following steps: respectively carrying out amplification culture of the strain number of a single bacterial specie on original strains of active probiotics for grass carps; mixing primary seed liquors of active probiotics for grass carps with same weight, inoculating the mixture in a seed tank medium to carry out multiple-strain mixed culture, then inoculating in a traditional Chinese medicine liquid medium to carry out a primary traditional Chinese medicine fermentation; and mixing the multiple-strain mixed traditional Chinese medicine fermentation liquor with isometric cob powder to obtain solid fermentation seeds, and then inoculating in a traditional Chinese medicine solid medium to carry out a secondary traditional Chinese medicine fermentation. According to the invention, the strain activity and colonization performance in the intestinal tracts of active probiotics in the grass carp feed additive are improved greatly, the prepared grass carp feed additive is rich in traditional Chinese medicine components, the active components with pharmacological effects in the traditional Chinese medicine components fully play the role, thus the performances of strengthening the body, treating diseases and promoting growth of the grass carp feed additive are improved, and the grass carp feed additive can effectively treat and prevent four diseases of grass carps, and furthermore completely displace antibiotics.