Primer and kit for detecting vesicular stomatitis viruses and preparation method of kit

Abstract

The invention discloses a primer for detecting vesicular stomatitis viruses, a detection kit composed of the primer and a preparation method of the detection kit. The primer for detecting the vesicular stomatitis viruses has gene sequences, namely SEQ ID No.1, SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4. A related experiment shows that a nucleic acid of the VSV can be specifically and rapidly amplified by the primer sequence and a specific band can be detected by nucleic acid electrophoresis; and meanwhile, the primer has the specificity. The primer can be applied to preparation of a GeXP diagnostic kit for rapidly identifying the VSV and applied to epidemiologic studies of vesicular stomatitis.

Description

technical field [0001] The invention relates to a primer, a detection kit and a preparation method that can be used to detect vesicular stomatitis virus. Background technique [0002] Vesicular stomatitis is a highly contagious infectious disease of various mammals caused by vesicular stomatitis virus (Vesiculovirus, VSV) of the family Rhabdoviridae (Rhabdoviridae), which can infect cattle, horses, etc. , pigs and other livestock. The disease can cause blisters and ulcers on the tongue, mouth, hooves and udders of infected animals, leading to loss of productivity in the affected areas, and is difficult to distinguish clinically from FMD. Vesicular stomatitis is not a new disease. As early as 1801, the epidemic of the disease appeared in the eastern United States, and then continued to spread in the United States. Since the 20th century, the disease has been popular in the Americas, Europe, and Africa, causing serious economic losses. In addition, the disease can also infec...

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Problems solved by technology

Lin et al. ( Lin F, Mackay DK, Knowles NJ. Detection of swine vesicular disease virus RNA by reverse transcription polymerase chain reaction [J]. J Virol Methods , 1997, 65 (1): 111.) established nested RT-PCR analysis This method is more sensitive than the virus isolation method from tissue culture. However, the nested RT-PCR system has a high risk of contamination and requires very strict conditions to reduce cross-contamination

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