Primer and kit for detecting vesicular stomatitis viruses and preparation method of kit

A technology for vesicular stomatitis and detection kits, which can be applied in biological testing, measuring devices, material inspection products, etc., and can solve problems such as large risk factors.

Active Publication Date: 2015-01-07
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Lin et al. ( Lin F, Mackay DK, Knowles NJ. Detection of swine vesicular disease virus RNA by reverse transcription polymerase chain reaction [J]. J Virol Methods , 1997, 65 (1): 111.) established nested RT-PCR anal...

Method used

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  • Primer and kit for detecting vesicular stomatitis viruses and preparation method of kit
  • Primer and kit for detecting vesicular stomatitis viruses and preparation method of kit
  • Primer and kit for detecting vesicular stomatitis viruses and preparation method of kit

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Embodiment Construction

[0018] The present invention will be explained in detail below in conjunction with the examples.

[0019] 1. Preparation of Sequences

[0020] According to the requirements of GeXP primer design and the genome of VSV published by NCBI, select the conserved region to design specific primers, and form specific chimeric primers by adding universal primers, while the universal primer sequences belong to non-biological nucleotide sequences, and synthesize Universal primer sequence, and a Cy5 fluorescent tag is added to the 5' end of the upstream universal primer. The obtained primer sequences are: SEQ ID No.1: AGGTGACACTATAGAATATGATACAGTACAATTATTTTGGGA, which is named VSV-F in the present invention; SEQ ID No.2: GTACGACTCACTATAGGGAGAGACTTTCTGTT AGGGATCTGG, which is named VSV-R in the present invention. Universal primers used in the present invention: SEQ ID No. 3: Cy5 AGGTGACACTATAGATA, named UWD-F in the present invention; and SEQ ID No. 4: GTACGACTCACTATAGGGA, named UEV-R in the...

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Abstract

The invention discloses a primer for detecting vesicular stomatitis viruses, a detection kit composed of the primer and a preparation method of the detection kit. The primer for detecting the vesicular stomatitis viruses has gene sequences, namely SEQ ID No.1, SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4. A related experiment shows that a nucleic acid of the VSV can be specifically and rapidly amplified by the primer sequence and a specific band can be detected by nucleic acid electrophoresis; and meanwhile, the primer has the specificity. The primer can be applied to preparation of a GeXP diagnostic kit for rapidly identifying the VSV and applied to epidemiologic studies of vesicular stomatitis.

Description

technical field [0001] The invention relates to a primer, a detection kit and a preparation method that can be used to detect vesicular stomatitis virus. Background technique [0002] Vesicular stomatitis is a highly contagious infectious disease of various mammals caused by vesicular stomatitis virus (Vesiculovirus, VSV) of the family Rhabdoviridae (Rhabdoviridae), which can infect cattle, horses, etc. , pigs and other livestock. The disease can cause blisters and ulcers on the tongue, mouth, hooves and udders of infected animals, leading to loss of productivity in the affected areas, and is difficult to distinguish clinically from FMD. Vesicular stomatitis is not a new disease. As early as 1801, the epidemic of the disease appeared in the eastern United States, and then continued to spread in the United States. Since the 20th century, the disease has been popular in the Americas, Europe, and Africa, causing serious economic losses. In addition, the disease can also infec...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/56983G01N2333/145
Inventor 张强卢昌赵志荀吴国华颜新敏李应国岳华周晓黎李健朱海霞代雪玲田波芦晓立高顺平王曼
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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