Gene chip for detecting 6 viruses of zoonosis-borne diseases and application thereof

A gene chip and virus technology, which is applied in the detection of 6 kinds of zoonotic virus gene chip and its application field, can solve the problems of epidemic obstacles, overlapping susceptibility animal spectrum, etc., and achieve good specificity and high sensitivity

Inactive Publication Date: 2012-10-10
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Rift Valley fever virus (RVFV), Nipah virus (NiV), foot-and-mouth disease virus (FMDV), vesicular stomatitis virus (VSV), hepatitis E virus (HEV) and rabies virus (RV) have been widely spread in recent years. , zoonotic viruses of relatively serious hazards have a great impact on social public health, and the spectrum of susceptible animals overlaps, causing great obstacles to the prevention and control of epidemics caused by these viruses

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gene chip for detecting 6 viruses of zoonosis-borne diseases and application thereof
  • Gene chip for detecting 6 viruses of zoonosis-borne diseases and application thereof
  • Gene chip for detecting 6 viruses of zoonosis-borne diseases and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, detect 6 kinds of zoonosis viruses with zoonosis virus gene chip

[0038] 1. Preparation of zoonotic virus gene chip

[0039] According to the gene sequences of RVFV, NIV, FMDV, VSV, RV and HEV viruses provided by GenBank, multiple sequence alignment analysis was carried out by bioanalysis software such as Bioedit and DNAman, combined with relevant literature [Han X Q, Lin X M, 1, Liu B H , et al. Simultaneously subtyping of all influenza A viruses using DNA microarrays[J]. Journal of Virological Methods, 2008, 152 () 17-121. Guillaume V, Lefeuvre A, Faure C, et al. Specific detection of Nipah virus using real- time RT-PCR[J].J Virol Methods 2004,120:229-237.], select the highly conserved regions of each virus to design probes, which are the non-structural protein 3ABCD region of FMDV, the N protein of RV and VSV region, the M protein region of RVFV, the P protein region of NIV and the structural protein ORF of HEV 2 area. Positive control probes and ne...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a gene chip for detecting six viruses of zoonosis-borne diseases and the application thereof. The gene chip for the viruses of the zoonosis-borne diseases is a gene chip for detecting at least one virus in six viruses of hepatitis E virus, Nipah virus, rabies virus, Rift Valley fever virus, foot and mouth disease virus and Vesicular stomatitis virus, and is fixed with a hepatitis E virus detection probe, a Nipah virus detection probe, a rabies virus detection probe, a Rift Valley fever virus detection probe, a foot and mouth disease virus detection probe and a Vesicular stomatitis virusdetection probe. The invention also discloses a method for detecting the Rift Valley fever virus, the Nipah virus, the foot and mouth disease virus, the Vesicular stomatitis virus, the hepatitis E virus and / or the rabies virus. The invention can stably and specifically implement generic detection of the six viruses of the zoonosis-borne diseases, has the advantages of simpleness, convenience, quickness, good specificity and high sensitivity, and can be used for detecting clinical samples.

Description

technical field [0001] The invention relates to a gene chip for detecting six kinds of zoonotic viruses and its application. Background technique [0002] Rift Valley fever virus (RVFV), Nipah virus (NiV), foot-and-mouth disease virus (FMDV), vesicular stomatitis virus (VSV), hepatitis E virus (HEV) and rabies virus (RV) have been widely spread in recent years. , zoonotic viruses of relatively serious hazards have a great impact on social public health, and the spectrum of susceptible animals overlaps, causing great obstacles to the prevention and control of epidemics caused by these viruses. Gene chip is a fast and high-throughput new technology developed in recent years. It uses in situ synthesis technology or micro spotting technology to arrange a large number of specific oligonucleotide fragments or target genes in an orderly and high-density manner on the surface of solid supports such as glass, silicon, nylon membranes, etc. dimensional DNA microarray. Hybridize the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68C40B40/06C12N15/11C12R1/93
Inventor 康晓平杨银辉孙庆歌李永强刘洪祝庆余吴东来户义孙婷婷
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap