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Gene chip for detecting 6 viruses of zoonosis-borne diseases and application thereof

A gene chip and virus technology, which is applied in the detection of 6 kinds of zoonotic virus gene chip and its application field, can solve the problems of epidemic obstacles, overlapping susceptibility animal spectrum, etc., and achieve good specificity and high sensitivity

Inactive Publication Date: 2012-10-10
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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Problems solved by technology

[0002] Rift Valley fever virus (RVFV), Nipah virus (NiV), foot-and-mouth disease virus (FMDV), vesicular stomatitis virus (VSV), hepatitis E virus (HEV) and rabies virus (RV) have been widely spread in recent years. , zoonotic viruses of relatively serious hazards have a great impact on social public health, and the spectrum of susceptible animals overlaps, causing great obstacles to the prevention and control of epidemics caused by these viruses

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  • Gene chip for detecting 6 viruses of zoonosis-borne diseases and application thereof
  • Gene chip for detecting 6 viruses of zoonosis-borne diseases and application thereof
  • Gene chip for detecting 6 viruses of zoonosis-borne diseases and application thereof

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Embodiment 1

[0037] Embodiment 1, detect 6 kinds of zoonosis viruses with zoonosis virus gene chip

[0038] 1. Preparation of zoonotic virus gene chip

[0039] According to the gene sequences of RVFV, NIV, FMDV, VSV, RV and HEV viruses provided by GenBank, multiple sequence alignment analysis was carried out by bioanalysis software such as Bioedit and DNAman, combined with relevant literature [Han X Q, Lin X M, 1, Liu B H , et al. Simultaneously subtyping of all influenza A viruses using DNA microarrays[J]. Journal of Virological Methods, 2008, 152 () 17-121. Guillaume V, Lefeuvre A, Faure C, et al. Specific detection of Nipah virus using real- time RT-PCR[J].J Virol Methods 2004,120:229-237.], select the highly conserved regions of each virus to design probes, which are the non-structural protein 3ABCD region of FMDV, the N protein of RV and VSV region, the M protein region of RVFV, the P protein region of NIV and the structural protein ORF of HEV 2 area. Positive control probes and ne...

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Abstract

The invention discloses a gene chip for detecting six viruses of zoonosis-borne diseases and the application thereof. The gene chip for the viruses of the zoonosis-borne diseases is a gene chip for detecting at least one virus in six viruses of hepatitis E virus, Nipah virus, rabies virus, Rift Valley fever virus, foot and mouth disease virus and Vesicular stomatitis virus, and is fixed with a hepatitis E virus detection probe, a Nipah virus detection probe, a rabies virus detection probe, a Rift Valley fever virus detection probe, a foot and mouth disease virus detection probe and a Vesicular stomatitis virusdetection probe. The invention also discloses a method for detecting the Rift Valley fever virus, the Nipah virus, the foot and mouth disease virus, the Vesicular stomatitis virus, the hepatitis E virus and / or the rabies virus. The invention can stably and specifically implement generic detection of the six viruses of the zoonosis-borne diseases, has the advantages of simpleness, convenience, quickness, good specificity and high sensitivity, and can be used for detecting clinical samples.

Description

technical field [0001] The invention relates to a gene chip for detecting six kinds of zoonotic viruses and its application. Background technique [0002] Rift Valley fever virus (RVFV), Nipah virus (NiV), foot-and-mouth disease virus (FMDV), vesicular stomatitis virus (VSV), hepatitis E virus (HEV) and rabies virus (RV) have been widely spread in recent years. , zoonotic viruses of relatively serious hazards have a great impact on social public health, and the spectrum of susceptible animals overlaps, causing great obstacles to the prevention and control of epidemics caused by these viruses. Gene chip is a fast and high-throughput new technology developed in recent years. It uses in situ synthesis technology or micro spotting technology to arrange a large number of specific oligonucleotide fragments or target genes in an orderly and high-density manner on the surface of solid supports such as glass, silicon, nylon membranes, etc. dimensional DNA microarray. Hybridize the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68C40B40/06C12N15/11C12R1/93
Inventor 康晓平杨银辉孙庆歌李永强刘洪祝庆余吴东来户义孙婷婷
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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