Gene chip for detecting 6 viruses of zoonosis-borne diseases and application thereof
A gene chip and virus technology, which is applied to the gene chip for detecting 6 zoonotic viruses and its application fields, can solve the problems of epidemic obstacles and overlapping susceptible animal spectrum, and achieve the effect of good specificity and high sensitivity
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[0037] Embodiment 1, detect 6 kinds of zoonosis viruses with zoonosis virus gene chip
[0038] 1. Preparation of zoonotic virus gene chip
[0039] According to the gene sequences of RVFV, NIV, FMDV, VSV, RV and HEV viruses provided by GenBank, multiple sequence alignment analysis was carried out by bioanalysis software such as Bioedit and DNAman, combined with relevant literature [Han X Q, Lin X M, 1, Liu B H , et al. Simultaneously subtyping of all influenza A viruses using DNA microarrays [J]. Journal of Virological Methods, 2008, 152 () 17-121. Guillaume V, Lefeuvre A, Faure C, et al. Specific detection of Nipah virus using real-time RT-PCR[J].J Virol Methods 2004,120:229-237.], select the highly conserved regions of each virus to design probes, which are the non-structural protein 3ABCD region of FMDV, RV and VSV N protein region, M protein region of RVFV, P protein region of NIV and structural protein ORF of HEV 2 area. Positive control probes and negative control prob...
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