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Gene chip of vesicular stomatitis viruses and/or swine vesicular disease viruses and reagent kit

A porcine vesicular disease virus and vesicular stomatitis technology, which is applied in biochemical equipment and methods, microbial measurement/testing, nucleotide library, etc., can solve the problems of long time required and achieve short time-consuming and high specificity Strong, highly sensitive effects

Inactive Publication Date: 2015-10-28
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the diagnosis of these two diseases mostly adopts isolation and identification and conventional serological methods, which takes a long time. There is also a method for identification by PCR technology, but there is no method that is highly operable and can simultaneously identify the two viruses.

Method used

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  • Gene chip of vesicular stomatitis viruses and/or swine vesicular disease viruses and reagent kit
  • Gene chip of vesicular stomatitis viruses and/or swine vesicular disease viruses and reagent kit
  • Gene chip of vesicular stomatitis viruses and/or swine vesicular disease viruses and reagent kit

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Embodiment 1

[0043] Embodiment 1 Detection method of the present invention

[0044] 1. Materials and instruments

[0045] The same experimental materials and instruments as mentioned above.

[0046] 2. Experimental method

[0047] 2.1 Design and synthesis of PCR primers and detection probes

[0048] 2.1.1 Primer design:

[0049] According to the VSV sequence and SVDV sequence published by NCBI, on the basis of fully comparing the homology, select the conserved region and use the software Primer Primer 5.0 to design primers and perform BLAST sequence comparison to initially verify the specificity of the primers. The primers used in this study are shown in Table 1, and the 5' end of the upstream primer of each pair of primers was connected with a Cy3 fluorescent group. All primers were synthesized by Sangon Bioengineering (Shanghai) Company.

[0050] Table 1 Primer sequence and target amplification product size

[0051]

[0052] 2.1.2 Probe design:

[0053] According to the amplified...

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Abstract

The invention discloses a gene chip of vesicular stomatitis viruses and / or swine vesicular disease viruses and a reagent kit. By means of the gene chip, the detection reagent kit and a detection method, the vesicular stomatitis viruses and / or the swine vesicular disease viruses can be accurately and effectively detected. Besides, the gene chip and the detection reagent kit are high in specificity and sensitivity, low in time consumption, high in detection speed and good in application prospect.

Description

technical field [0001] The invention relates to a gene chip and a detection method for detecting vesicular stomatitis virus and / or porcine vesicular disease virus. Background technique [0002] Vesicular stomatitis (VS) and swine vesicular disease (SWD) are caused by vesicular stomatitis virus (VSV) and swine vesicular disease virus (SVDV), respectively. Acute and highly contagious infectious diseases of mammals. These two types of viruses can infect pigs with a very high incidence and can form a large-scale epidemic, which can cause serious public health problems. Both types of viruses have been approved by the World Organization for Animal Health (OIE) as a notifiable animal disease. Both of these diseases are characterized by epithelial blisters on the tongue, lips, oral mucosa, teats, and hooves as the main symptom. Therefore, it is impossible to distinguish these three diseases in terms of clinical symptoms, and differential diagnosis must be made through etiology. ...

Claims

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Application Information

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IPC IPC(8): C40B40/06C12Q1/70C12Q1/68
Inventor 文心田黄小波欧阳达曹三杰伍锐文翼平
Owner SICHUAN AGRI UNIV
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