Vesicular disease detection kit

Abstract

The invention discloses primers which can be used for detecting aftosa (type A, type O, and type Asia 1), swine pox and vesicular stomatitis at the same time, a kit consisting of the primers and a preparation method, and a use method of the kit for a dis-illness diagnosis purpose. The invention provides 10 gene sequences of the primers for detecting type A, type O, and type Asia 1 viruses of aftosa, and the kit further comprises two general primers besides the ten primers. Related experiments show that sensitivity of the use method in the invention is higher than sensitivity of general fluorogenic quantitative PCR technology. Compared with the fluorogenic quantitative PCR technology, the detection time is greatly saved; augmentation of non-specific products is avoided; phenomenon of preferences of primers is effectively avoided; and detection sensitivity is improved.

Description

technical field [0001] The present invention relates to a kind of primer that can be used for detecting a variety of pathogenic microorganisms, a kit made of these primers and a preparation method, and a method for using the kit for non-disease diagnosis purposes. Primers for the detection of foot-and-mouth disease (types A, O and Asia 1), porcine vesicular disease and vesicular stomatitis, kits and preparation methods consisting of these primers, and the use of such kits for non-disease diagnosis purposes method. Background technique [0002] Foot-and-Mouth disease (FMD) is an acute contagious disease of cloven-hoofed animals caused by Picornaviridae (Foot-and-Mouth disease virus, FMDV). There are 7 serotypes including type C, type C, SAT Ⅰ (South African type Ⅰ), SAT Ⅱ (South African type Ⅱ), SAT Ⅲ (South African type Ⅲ), and Asia 1 (Asian 1 type). Swine vesicular disease (SVD) is an acute contagious disease caused by Swine vesicular disease virus (SVDV) of the family Pi...

AI Technical Summary

Problems solved by technology

Conventional diagnostic methods, such as virus isolation, serological tests, animal tests, etc., are time-consuming and labor-intensive, or have low specificity and sensitivity, and cannot meet the requirements of rapid and accurate diagnosis

Existing RT-PCR, competitive PCR and ELISA-PCR methods overcome the shortcomings of these methods and can be used for the diagnosis of these three diseases, but they also have disadvantages such as time-consuming, easy contamination and small number of samples per test.

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