Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Kit for detecting vesicular stomatitis antibody and preparation method thereof

A technology of vesicular stomatitis and kit, which is applied in the field of vesicular stomatitis virus antibody detection kit and its preparation, and can solve the problems of complex, cumbersome and time-consuming tests

Inactive Publication Date: 2010-01-13
花群义
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a test kit for detecting vesicular stomatitis virus antibody and its preparation method in view of the problems of complicated, troublesome, unsafe and time-consuming tests in the diagnosis of vesicular stomatitis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting vesicular stomatitis antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] The invention provides a kit for detecting vesicular stomatitis virus antibody. The kit includes reagent I and reagent II placed separately, wherein reagent I is an enzyme-linked immunosorbent assay assay plate coated with vesicular stomatitis virus antigen, and reagent II It is a monoclonal antibody IgG-HRP enzyme conjugate, wherein the monoclonal antibody IgG-HRP enzyme conjugate is a mouse specific monoclonal antibody against vesicular stomatitis virus labeled with horseradish peroxidase.

[0033] In addition, in the above kit, positive serum, negative serum, 20-fold concentrated washing solution, 10-fold concentrated washing solution, substrate I, substrate II, substrate III and stop solution may also be included separately. The 20-fold concentrated washing solution is 20-fold concentrated 0.01mol / L pH 7.4 PBS / PBST containing 0.05% Tween-20; the 10-fold concentrated dilution is 10-fold concentrated PBST containing 1% gelatin; the substrate I is pH 6 .0 acetic acid-c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a kit for detecting vesicular stomatitis antibody and a preparation method thereof. The kit for detecting vesicular stomatitis antibody comprises a reagent I and a reagent II placed respectively, wherein the reagent I is a vesicular stomatitis virus antihelion peridium enzyme linked immunosorbent assay plate and the reagent II is monoclonal antibody IgG-HRP enzyme conjugates, wherein the monoclonal antibody IgG-HRP enzyme conjugates is idiocrasy monoclonal antibody of mouse-anti vesicular stomatitis virus of horse radish peroxidase mark. The invention also provides a preparation method of the kit. The kit adopts competitive ELISA to detect the vesicular stomatitis in serum, and can be used for rapid diagnosis, detection, quarantine and epidemiological investigation of vesicular stomatitis.

Description

technical field [0001] The invention relates to the field of virus antibody detection, in particular to a kit for detecting vesicular stomatitis virus antibody and a preparation method thereof. Background technique [0002] Vesicular stomatitis is a major zoonotic disease caused by vesicular stomatitis virus (Vesicular stomatitis Virus, VSV) of Rhabdoviridae genus Vesiculovirus, currently Indiana (Indiana, IN type) and New Jersey (New Jersey, NJ type) two serotypes. Cattle, pigs, horses, sheep, and a variety of other wild animals can be infected with vesicular stomatitis virus, which can cause flu- and dengue-like symptoms in humans. The VSV genome encodes five viral proteins, including N, NS, M, G, and L, among which the N protein is the main protein of the nucleocapsid of the virus and is the group-specific antigen of VSV. [0003] Because the clinical symptoms of vesicular stomatitis are similar to those of foot-and-mouth disease (FMD), the existence and prevalence of t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/577G01N33/569G01N33/535C12N15/06C12P21/08
Inventor 花群义杨俊兴张彩虹阮周曦曾少灵曹琛福林庆燕秦智锋陈书琨陶虹
Owner 花群义
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com