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Kit for detecting vesicular stomatitis antibody and preparation method thereof

A technology of vesicular stomatitis and kit, which is applied in the field of vesicular stomatitis virus antibody detection kit and its preparation, and can solve the problems of complex, cumbersome and time-consuming tests

Inactive Publication Date: 2010-01-13
花群义
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a test kit for detecting vesicular stomatitis virus antibody and its preparation method in view of the problems of complicated, troublesome, unsafe and time-consuming tests in the diagnosis of vesicular stomatitis

Method used

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  • Kit for detecting vesicular stomatitis antibody and preparation method thereof

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Embodiment Construction

[0032] The invention provides a kit for detecting vesicular stomatitis virus antibody. The kit includes reagent I and reagent II placed separately, wherein reagent I is an enzyme-linked immunosorbent assay assay plate coated with vesicular stomatitis virus antigen, and reagent II It is a monoclonal antibody IgG-HRP enzyme conjugate, wherein the monoclonal antibody IgG-HRP enzyme conjugate is a mouse specific monoclonal antibody against vesicular stomatitis virus labeled with horseradish peroxidase.

[0033] In addition, in the above kit, positive serum, negative serum, 20-fold concentrated washing solution, 10-fold concentrated washing solution, substrate I, substrate II, substrate III and stop solution may also be included separately. The 20-fold concentrated washing solution is 20-fold concentrated 0.01mol / L pH 7.4 PBS / PBST containing 0.05% Tween-20; the 10-fold concentrated dilution is 10-fold concentrated PBST containing 1% gelatin; the substrate I is pH 6 .0 acetic acid-c...

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Abstract

The invention relates to a kit for detecting vesicular stomatitis antibody and a preparation method thereof. The kit for detecting vesicular stomatitis antibody comprises a reagent I and a reagent II placed respectively, wherein the reagent I is a vesicular stomatitis virus antihelion peridium enzyme linked immunosorbent assay plate and the reagent II is monoclonal antibody IgG-HRP enzyme conjugates, wherein the monoclonal antibody IgG-HRP enzyme conjugates is idiocrasy monoclonal antibody of mouse-anti vesicular stomatitis virus of horse radish peroxidase mark. The invention also provides a preparation method of the kit. The kit adopts competitive ELISA to detect the vesicular stomatitis in serum, and can be used for rapid diagnosis, detection, quarantine and epidemiological investigation of vesicular stomatitis.

Description

technical field [0001] The invention relates to the field of virus antibody detection, in particular to a kit for detecting vesicular stomatitis virus antibody and a preparation method thereof. Background technique [0002] Vesicular stomatitis is a major zoonotic disease caused by vesicular stomatitis virus (Vesicular stomatitis Virus, VSV) of Rhabdoviridae genus Vesiculovirus, currently Indiana (Indiana, IN type) and New Jersey (New Jersey, NJ type) two serotypes. Cattle, pigs, horses, sheep, and a variety of other wild animals can be infected with vesicular stomatitis virus, which can cause flu- and dengue-like symptoms in humans. The VSV genome encodes five viral proteins, including N, NS, M, G, and L, among which the N protein is the main protein of the nucleocapsid of the virus and is the group-specific antigen of VSV. [0003] Because the clinical symptoms of vesicular stomatitis are similar to those of foot-and-mouth disease (FMD), the existence and prevalence of t...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/535C12N15/06C12P21/08
Inventor 花群义杨俊兴张彩虹阮周曦曾少灵曹琛福林庆燕秦智锋陈书琨陶虹
Owner 花群义
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