Reagent box for detecting aftosa A type, O type and Asia 1 type viruses and preparing method thereof
A detection kit and technology of foot-and-mouth disease virus, which is applied to the primers of type O and Asia 1 virus, and the field of detection of type A of foot-and-mouth disease, which can solve the problem of inability to distinguish serotypes
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0038] The present invention will be explained in detail below in conjunction with the examples.
[0039] 1. Preparation of Sequences
[0040] According to the GeXP primer design requirements and the genomes of FMDV-A, FMDV-O, and FMDV-Asia 1 published by NCBI, the conserved regions were selected to design specific primers, and specific chimeric primers were formed by adding universal primers. It is a non-biologically derived nucleotide sequence. In addition, a universal primer sequence is synthesized, and a Cy5 fluorescent label is added to the 5' end of the upstream universal primer.
[0041] 2. Viral Genome Extraction
[0042] After the monolayer cells grow to more than 80% confluent, inoculate the virus, incubate at 37°C for 30 minutes, and discard the venom. Add cell maintenance solution, 37°C 5% CO 2 Cultivate, observe cytopathic changes (CPE) regularly, and collect the poison after more than 90% of the cells appear CPE. The virus was repeatedly frozen and thawed th...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com