Primer, detection kit and preparation method for detecting porcine vesicular disease virus

Abstract

The invention discloses a primer and a detection kit for detecting swine vesicular disease virus and a preparation method thereof. The primer for detecting the swine vesicular disease virus comprises the following gene sequences: SEQIDNo. 1 (SVDV-F), SEQIDNo. 2(SVDV-R), SEQIDNo. 3 (UWD-F) and SEQIDNo. 4 (UEV-R). Related experiments show that the primer sequences disclosed by the invention can be used for specifically and rapidly amplifying nucleic acid of vesicular stomatitis virus, can be used for detecting specific bands by virtue of nucleic acid electrophoresis, and have specificity. The primer and detection kit disclosed by the invention can be applied to the preparation of a GeXP diagnostic kit for rapidly identifying the swine vesicular disease virus, and can be applied to the research of swine vesicular disease epidemiology.

Description

technical field [0001] The invention relates to a primer, a detection kit and a preparation method for detecting porcine vesicular disease virus. Background technique [0002] Swine vesicular disease is an acute and highly contagious infectious disease of pigs caused by Swinevesicular disease virus (SVDV) of the family Picornaviridae (Picornaviridae) Enterovirus. High, seriously hindering the exchange and international trade of pig products, and can cause serious public health problems, so the World Organization for Animal Health (IOE) listed it as a class A infectious disease of animals. The disease can cause blisters and ulcers on the tongue, mouth, hooves and udders of infected animals, leading to loss of productivity in the affected areas, and is difficult to distinguish clinically from FMD. The disease first occurred in Italy in 1966, and the virus was extracted and identified two years later. Subsequently, the disease appeared in many countries and regions one after ...

AI Technical Summary

Problems solved by technology

But after all, as a common method, in terms of sensitivity, SVDVRNA can only be detected after 10-fold serial dilution, and the result is that SVDVRNA can only reach 10 -4 Positives can only be detected when the dilution is above, indicating that the sensitivity is better than ELISA, but compared with more sensitive techniques, the sensitivity is still not high enough

Therefore, it is also easy to miss detection in clinical use, and due to the need for agarose gel electrophoresis analysis, large-scale operation is difficult

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