Primer, detection kit and preparation method for detecting porcine vesicular disease virus

A swine vesicular disease virus and detection kit technology, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as insufficient sensitivity, difficulty in large-scale operation, and missed detection.
CN104342501BActive Publication Date: 2016-06-08LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Publication Date
2016-06-08

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Abstract

The invention discloses a primer and a detection kit for detecting swine vesicular disease virus and a preparation method thereof. The primer for detecting the swine vesicular disease virus comprises the following gene sequences: SEQIDNo. 1 (SVDV-F), SEQIDNo. 2(SVDV-R), SEQIDNo. 3 (UWD-F) and SEQIDNo. 4 (UEV-R). Related experiments show that the primer sequences disclosed by the invention can be used for specifically and rapidly amplifying nucleic acid of vesicular stomatitis virus, can be used for detecting specific bands by virtue of nucleic acid electrophoresis, and have specificity. The primer and detection kit disclosed by the invention can be applied to the preparation of a GeXP diagnostic kit for rapidly identifying the swine vesicular disease virus, and can be applied to the research of swine vesicular disease epidemiology.
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Description

technical field

[0001] The invention relates to a primer, a detection kit and a preparation method for detecting porcine vesicular disease virus. Background technique

[0002] Swine vesicular disease is an acute and highly contagious infectious disease of pigs caused by Swinevesicular disease virus (SVDV) of the family Picornaviridae (Picornaviridae) Enterovirus. High, seriously hindering the exchange and international trade of pig products, and can cause serious public health problems, so the World Organization for Animal Health (IOE) listed it as a class A infectious disease of animals. The disease can cause blisters and ulcers on the tongue, mouth, hooves and udders of infected animals, leading to loss of productivity in the affected areas, and is difficult to distinguish clinically from FMD. The disease first occurred in Italy in 1966, and the virus was extracted and identified two years later. Subsequently, the disease appeared in many countries and regions one after ...

Claims

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