A swine vesicular disease detection kit

A detection kit and technology for porcine vesicular disease, applied in the field of porcine vesicular disease detection kits, can solve the problems of rapid and accurate diagnosis, low specificity and sensitivity, and the number of easily contaminated samples, etc., and achieve intuitive results , strong specificity and easy operation

Active Publication Date: 2014-10-15
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Conventional diagnostic methods, such as virus isolation, serological tests, animal tests, etc., are time-consuming and laborious, or have low specificity and sensitivity, and cannot meet the requirements of rapid and accurate diagnosis
Existing RT-PCR, competitive PCR and ELISA-PCR methods overcome the shortcomings of these methods and can be used for the diagnosis of porcine vesicular disease, but they also have disadvantages such as time-consuming, easy to contaminate, and a small number of samples per test.

Method used

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  • A swine vesicular disease detection kit
  • A swine vesicular disease detection kit
  • A swine vesicular disease detection kit

Examples

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Embodiment Construction

[0024] The following examples will further illustrate the present invention:

[0025] The method in this embodiment is a reverse transcription loop-mediated isothermal amplification method for detecting porcine vesicular disease. The method is mainly composed of 10×LAMP reaction buffer, a pair of outer primers and a pair of inner primers, Bst DNA polymerase, AMV reverse transcriptase, dNTPs, magnesium sulfate (MgS0 4 ), Betaine and sterilized double distilled water.

[0026] According to the published nucleotide sequence of the swine vesicular disease HK'70 strain (GenBank accession number: AY429470), using LAMP online primer design software PrimerExplorer software V4 (http: / / primerexplorer.jp / elamp4.0.0 / index .html), designed and synthesized a set of primers, including two outer primers and two inner primers. The specific primer sequences are as follows:

[0027] Upstream outer primer: GCAGGTTTTACACGCTCGA

[0028] Downstream outer primer: TGGCACACCCCATCTCTG

[0029] Upstream inner pr...

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Abstract

The present invention discloses a kit for pork blister detection.The invention of the pork blister test kit includes two pairs of primers, namely the upstream outer primer SEQ№1, the downstream outer primer SEQ№2, the upstream inner primer SEQ№3, and the downstream inner primer SEQ№4.Compared with the existing technology, the present invention does not require any special equipment when testing. The detection sensitivity is higher than the existing technology.

Description

technical field [0001] The invention relates to a reagent kit for detecting pathogenic microorganisms, which belongs to the technical field of pathogenic microorganism detection, in particular to a reagent kit for detecting swine blister disease. Background technique [0002] Swine vesicular disease (SVD) is an acute contagious disease caused by Swine vesicular disease virus (SVDV) of the family Picornaviridae. The disease was first discovered in a pig farm in Italy in 1966, and then spread in many countries in Europe, causing serious economic losses and leading to chaos in the world meat market. The World Health Organization (OIE) lists it as a class A infectious disease, and the Ministry of Agriculture of my country lists it as a class I animal disease. Rapid and reliable diagnosis is essential for the control and eradication of swine vesicular disease, and methods for accurate detection of low levels of porcine vesicular disease virus in meat products, recessively infecte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68
Inventor 张强朱海霞吴国华赵志荀颜新敏李健芦晓立于少雄卢昌王曼朱彩珠
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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