Natural anti- virus and composition comprising thereof
A technology of composition and antiviral agent, which is applied in the field of natural antiviral agent composition, natural antiviral agent and compositions including it, and can solve problems such as destroying the immune system and destroying the chicken body
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[0034] Preparation of Kimchi Lactic Acid Bacteria Culture Solution
[0035] Among the kimchi lactic acid bacteria, Leuconostoc kimchi (KCTC2386), Leuconostoc enterica subsp. enterica (KCTC3530), Leuconostoc citreum (KCTC3524), Leuconostoc lactis (KCTC3528), Lactobacillus plantarum (KCCM11322), and Weissella koreensis (KCTC3746) was used to prepare the culture medium.
[0036] First, 100 ml of MRS (Difco) broth was autoclaved at 121° C. for 15 minutes, and Kimchi lactic acid bacteria were inoculated into the broth, and shake cultured at 30° C. for 16 hours.
[0037] After the obtained culture was centrifuged at 5000×g for 3 min, the supernatant was filtered through a membrane filter having a pore size of 0.22 to obtain a sterile solution.
[0038] Subsequently, this sterile solution was passed through a membrane with a cut-off of 10,000 Da. The resulting filtrate was concentrated using a membrane with a 1,000 Da cut-off. The concentrate thus obtained was filtered again throu...
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