Artificial synthetic porcine interferon and method for preparing porcine interferon

Abstract

The invention relates to a synthetic porcine interferon gene, a carrier-host cell containing same and a method for preparing porcine interferon. The synthetic porcine interferon gene has an SEQ ID NO.1 nucleotide sequence shown in a figure 1. The preparation method comprises the steps of synthesizing a synthetic porcine alpha-IFN gene and the carrier-host cell containing the gene, recombining pichia for fermentation to produce the porcine interferon and purifying the obtained porcine alpha interferon. An expression system has the characteristics of high expressivity, high stability and high secretion. The pichia as host bacteria is small in the secretion of own protein, large in the secretion of high-activity foreign protein and beneficial to downstream separation and purification, is a eukaryotic expression system suitable for expressing foreign gene, and is beneficial to large-scale batch production.

Description

technical field [0001] The invention belongs to the field of microbial engineering, and in particular relates to an artificially synthesized porcine interferon gene, a carrier containing the gene, a host cell and a method for preparing porcine interferon. Background technique [0002] Interferon is a soluble glycoprotein produced by a variety of cells with a wide range of antiviral, antitumor and immunomodulatory effects. At present, the mass production of porcine interferon mainly includes cell induction culture method and gene recombination method using Escherichia coli as the expression system. The former method uses animal cells as raw materials, the production process is complicated, the production cost is high, and the product has the risk of carrying exogenous viruses and other pathogenic microorganisms. Although the latter method is more advanced than the former one, and the production cost is greatly reduced, its process is still relatively complicated. The express...

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Problems solved by technology

The former method uses animal cells as raw materials, the production process is complicated, the production cost is high, and the product has the risk of carrying exogenous viruses and other pathogenic microorganisms

Although the latter method is more advanced than the former one, and the production cost is greatly reduced, its process is still relatively complicated. The expression product of genetically engineered positive E. Renaturation and other processes, and the renaturation rate is low, so the specific activity is also low

In addition, the purity of the product is not high, and it contains other miscellaneous proteins, and the separation and purification are complicated, requiring expensive monoclonal antibody columns

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