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A kind of preparation method of the powdery algae protoplast

A technology of protoplast and silk powder is applied in the field of plant cell engineering to achieve the effects of good integrity, high yield and simple method

Active Publication Date: 2020-05-05
HAINAN ACADEMY OF OCEAN & FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problem in the prior art that there is no method for preparing the protoplasts of P.

Method used

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  • A kind of preparation method of the powdery algae protoplast

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preparation example Construction

[0039] 3.1 Preparation of protoplasts

[0040] Taking 2g of the young leaves of S. rotundum as an example, the specific steps are as follows:

[0041] S1: select the young leaves of Trichophyllum rotifera, wash them with sterile seawater, and raise them temporarily to obtain raw material 1;

[0042] S2: Soak the raw material 1 obtained in S1 in the pretreatment solution to obtain raw material 2;

[0043] S3: Take out the raw material 2 obtained in S2, after absorbing the water, cut the raw material 2 into 0.2cm 2 Add 10mL enzyme solution for enzymolysis to obtain enzymolysis tissue;

[0044] S4: Filter the enzymolyzed tissue obtained in S3 with a 200-mesh sieve to obtain a filtrate;

[0045] S5: Centrifuge the filtrate obtained in S4 at 70×g for 5 min, discard the supernatant, suspend the precipitate with W5 solution, centrifuge, discard the supernatant, and obtain the precipitate;

[0046] S6: Suspend the precipitate obtained in S5 with 2 mL of MMG solution to obtain the ...

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Abstract

The invention discloses a preparation method of round leaf zannichelliaceae cymodocea rotundta protoplast, and belongs to the field of plant cell engineering. The preparation method comprises the stepS1 of taking spires of round leaf zannichelliaceae cymodocea rotundta, cleaning the spires with sterile seawater, and then performing temporary culture to obtain a raw material 1; the step S2 of immersing the raw material 1 obtained in the step S1 into a pretreatment solution to obtain a raw material 2; the step S3 of taking out the raw material 2 obtained in the step S2, removing the moisture, cutting the raw material into pieces and adding an enzyme solution for enzymatic hydrolysis to obtain an enzymatic hydrolysis tissue; the step S4 of filtering the enzymatic hydrolysis tissue obtained in the step S3 to obtain a filtrate; the step S5 of centrifuging the filtrate obtained in the step S4, abandoning a supernatant, conducting suspension and precipitation with a W5 solution, centrifugingand abandoning the supernatant to obtain a precipitate; the step S6 of suspending the precipitate obtained in the step S5 in an MMG solution to obtain the round leaf zannichelliaceae cymodocea rotundta protoplast. The round leaf zannichelliaceae cymodocea rotundta protoplast obtained by the method has high yield and high survival rate.

Description

technical field [0001] The invention belongs to the field of plant cell engineering, and in particular relates to a method for preparing the protoplast of Trichophyllum rotundum. Background technique [0002] Seagrass is a large monocotyledonous submerged plant that lives in shallow seas in tropical and temperate seas, and belongs to the order Marzophyta. They have the general characteristics of higher plants and are able to complete their life cycle in water. The primary productivity of seagrass is very high, and it is an important component of the shallow water ecosystem - seagrass field. The seagrass field resources are unique, and its ecological and economic value is mainly manifested in: (1) Seagrass can accelerate the settlement of suspended solids in seawater, absorb nutrients and heavy metals in seawater, thereby improving water quality; (2) Seaweed can slow down water flow, thereby Increase the settlement of suspended solids in the water to achieve the effect of s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 王道儒刘涛刘维蔡泽富吴钟解陈石泉
Owner HAINAN ACADEMY OF OCEAN & FISHERIES SCI
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