36results about How to "Stable osmotic pressure" patented technology

The invention discloses a serum-free cryopreservation solution for immune cells, which is characterized in that it comprises a basal medium and a cell cryopreservation protective agent, and the cell cryopreservation protective agent is composed of the following raw materials: polyethylene glycol, nonylphenol poly Oxyethylene ether-10, acacia polysaccharide, sodium carboxymethylcellulose, polyglyceryl fatty acid ester, bamboo leaf flavone. In the present invention, polyethylene glycol and nonylphenol polyoxyethylene ether-10 are used to replace DMSO to change the permeability of the cell membrane and at the same time protect the cell membrane, so that the free water in the cell can be excluded from the cell and reduce the risk of cryopreservation. The formation of ice crystals in the cells during the process damages the cells. Acacia polysaccharides, sodium carboxymethylcellulose, and polyglycerol fatty acid esters are added as osmotic pressure stabilizers to maintain a stable osmotic pressure in the extracellular environment. Added with bamboo leaf flavonoids, it helps cells recover quickly after recovery. The invention also provides a cryopreservation method for immune cells, which is simple and convenient, and meets the demands for immune cells in scientific research, medical treatment and other fields.

The invention provides mesenchymal stem cells combined with traditional Chinese medicines for activating amplification, and a preparation method of the mesenchymal stem cells comprises the following steps: (1) pretreatment: digesting tissue pieces with pancreatin and IV collagenase, separating primary mesenchymal stem cells; (2) culture: adding the cells obtained in the step (1) to a low sugar DMEM culture medium containing components of 50-80mg / mL hemianthus micranthemoides extract, 50-80mg / mL Chinese gall extract, 1.5-3mmol / mL L-glutamine and 1.5-3ng / mL bFGF, culturing in a wet incubator of 37 DEG C with 5% CO2, changing the culture medium once every 3-4 days; and (3) collection of cells and subculture. Shown by in vitro tests, the traditional Chinese medicines activated mesenchymal stem cells can effectively inhibit reproduction of HBV and promote repair of damaged hepatic cells.

The invention discloses a preparation method of round leaf zannichelliaceae cymodocea rotundta protoplast, and belongs to the field of plant cell engineering. The preparation method comprises the stepS1 of taking spires of round leaf zannichelliaceae cymodocea rotundta, cleaning the spires with sterile seawater, and then performing temporary culture to obtain a raw material 1; the step S2 of immersing the raw material 1 obtained in the step S1 into a pretreatment solution to obtain a raw material 2; the step S3 of taking out the raw material 2 obtained in the step S2, removing the moisture, cutting the raw material into pieces and adding an enzyme solution for enzymatic hydrolysis to obtain an enzymatic hydrolysis tissue; the step S4 of filtering the enzymatic hydrolysis tissue obtained in the step S3 to obtain a filtrate; the step S5 of centrifuging the filtrate obtained in the step S4, abandoning a supernatant, conducting suspension and precipitation with a W5 solution, centrifugingand abandoning the supernatant to obtain a precipitate; the step S6 of suspending the precipitate obtained in the step S5 in an MMG solution to obtain the round leaf zannichelliaceae cymodocea rotundta protoplast. The round leaf zannichelliaceae cymodocea rotundta protoplast obtained by the method has high yield and high survival rate.