36results about How to "Stable osmotic pressure" patented technology

The invention discloses a culture medium for expanding human mesenchymal stem cells, which comprises a basic medium and an additive added to the medium, and the additive includes the following components at final concentrations: linoleic acid 2‑10 μL/mL, human Source AB type serum 5‑15 μL/mL, human transferrin 5‑15 μg/mL, etc. Using the culture medium to expand human mesenchymal stem cells includes the following steps: (1) isolating human mesenchymal stem cells from umbilical cord or placenta, or isolating mononuclear cells from bone marrow blood, umbilical cord blood or placental blood, and placing them in the culture medium (2) Place the cells separated and purified in step (1) into fresh medium for subculture. The invention adopts the culture medium of specific components and the expansion method, which can effectively speed up the cell expansion speed, shorten the expansion time, maintain the uniform shape of the cells after passaging, and reduce their differentiation, thereby ensuring the safety of human mesenchymal stem cells sex.

It is intended to provide a safe and highly stable peritoneal dialysate which causes neither peritoneal membrane disorders nor peritoneal sclerosis in frequently repeated peritoneal dialysis treatment, can protect the residual renal functions in chronic renal failure and inhibit the progress of renal damage over a long period of time, and enables peritoneal dialysis treatment in a diabetic patient. A peritoneal dialysate which contains 0.05 to 3.5 w/v% of taurine and 0.1 to 6.5 w/v% of trehalose as osmotic agents and having a pH value regulated to 6.5 to 7.5.

The invention provides a method and system for testing the rock permeability in a dynamic load disturbance process, and belongs to the technical field of geotechnical engineering. The method for testing the rock permeability in the dynamic load disturbance process comprises the steps of: selecting a sample, saturating the sample, applying confining pressure, applying dynamic load, applying hydraulic pressure, and calculating the permeability. The test system comprises a confining pressure chamber, a confining pressure loading device, a power loading device and a pore pressure loading device; the confining pressure chamber is hollow; the confining pressure loading device is connected with the confining pressure chamber; the power loading device and the confining pressure chamber are in abutting joint; the power loading device is used for applying dynamic load onto a rock sample along the axial direction of the rock sample; the pore pressure loading device comprises a pipeline and a hydraulic pump; the pipeline is separately communicated with the hydraulic pump and the confining pressure chamber; and the hydraulic pump is used for pumping liquid to the end part of the rock sample. Bymeans of the method and the system in the invention, the technical problems that the stress state of the rock sample is single and the security of underground engineering under the combined action ofdynamic load and water pressure cannot be reflected in the prior art can be solved.

The invention discloses a compound premix capable of enhancing the content of proteins in muscles of a grass carp. The compound premix consists of 10000g of the raw materials in weight mixing ratio: 1000g of compound vitamins, 5000g of compound mineral elements, 1000g of Beta-dextran premix agents, 500 to 1000g of taurine and unite bran. The addition of the compound premix of the invention in the formula feed for grass carp takes a weight proportion of 1 percent to ensure that the condition factor of the cultivated grass carp is larger than 2, the organ body weight proportion is smaller than the weight ratio, 11percent, and the content of the proteins in the muscles of the fish body is larger than 22 percent. The invention has the advantages that the grass carp cultivated using the compound feed of the premix has enhanced the edible part and the better meat quality. Saving the resources, the compound premix of the invention is beneficial to the environmental protection and is more beneficial to the increasing demand by the national citizens for the quality and safety of the aquatic foods.

The invention relates to a formula of feed for livestock, and particularly discloses a compound premix for livestock. The pecking feed part of the compound premix is prepared from the following components: barley, water, L-lysine hydrochloride, DL-methionine, ferrous sulfate, zinc sulfate, copper chloride hydroxide, manganese sulfate, sodium selenite, calcium iodate, vitamin complex for chicken, mildew preventive, magnesium sulfate, sodium bicarbonate, potassium chloride, calcium formate, calcium hydrophosphate, ethoxyquinoline, dextrin and starch. The raw materials are uniformly mixed and pressed into cake to obtain the compound premix. The compound premix has the advantages that (1) natural habits of livestock for pecking and ingesting can be met, and pickingill of chicken can be prevented; (2) the components are reasonably proportioned, the production performance of feeding chicken can be remarkably improved; (3) the ingesting time of feeding chicken can be effectively prolonged, and nutritional substances can be effectively absorbed; (4) the components in the feed cannot be layered due to proportion difference in the feeding chicken pecking process.

The invention provides a de-host extraction method for a sputum pathogenic microorganism metagenome. The method comprises steps of sputum pretreatment, differential lysis to remove the host and nucleic acid extraction. The invention combines DTT and protease to quickly homogenize sputum, and then uses surfactant to differentially lyse cells, reduces interference of human genes, realizes enrichmentof microbial genome, and effectively improves detection rate of metagenomic pathogens in sputum samples.

The invention relates to a blastocyst vitrification refrigerating fluid and a refrigerating method. The vitrification refrigerating fluid comprises an equilibrium liquid, a refrigerating fluid 1 and arefrigerating fluid 2; wherein the equilibrium liquid comprises a basic equilibrium liquid and a protein additive; the refrigerating fluid 1 comprises polyvinylpyrrolidone, ethylene glycol, dimethylsulfoxide and the equilibrium liquid; the refrigerating fluid 2 comprises polyvinylpyrrolidone, ethylene glycol, dimethyl sulfoxide, trehalose, polysucrose and the equilibrium liquid. The vitrification refrigerating fluid provided by the invention can significantly improve the freeze-thaw survival rate and the recruitment rate of blastocysts, and is short in time consumption.

The invention relates to the medical field, particularly to a composition and its use, an umbilical cord preservation preparation and a preparation method thereof. According to the invention, physiological saline is taken as the basic ingredient of umbilical cord preservation liquid, umbilical cord blood plasma is used as the nutritional ingredient source, as a natural substance, umbilical cord blood plasma not only provides multiple nutritional ingredients, but also can maximumly maintain the umbilical cord vitality in order to maintain a microenvironment similar to that in vivo. Lactobionic acid, hydroxyethyl starch and other macromolecular substances are added to avoid swelling death of the umbilical cord in the preservation process. The preservation liquid can be used for transportation and preservation of the preparation at low temperature. Thus, adequate nutrients are supplied to in vitro umbilical cord, and also the seed cell vitality, the original cell morphology and biological characteristics are well maintained.

The invention discloses a seedling raising substrate for cultivating salt resistant plant seedlings, and a preparation method and application of the seedling raising substrate for cultivating salt resistant plant seedlings. The seedling raising substrate for cultivating salt resistant plant seedlings is prepared from mushroom residues, livestock and poultry excrement and urine, vinegar residues, gypsum residues, vermiculite, functional microorganisms and plant nutrient substances. The preparation method comprises the steps of preparing thoroughly decomposed mushroom residues through stacking fermentation of the mushroom residues, the livestock and poultry excrement and urine and the vinegar residues, preparing plant nutrition element enveloping bodies through entrapping potassium nitrate and magnesium sulphate with urea-formaldehyde resins, preparing microorganism enveloping bodies through entrapping functional microorganisms of trichoderma harzinum T83 and bacillus amyloliquefaciens IAE with polyglutamic acid, and preparing the substrate through solid fermentation and compounding of raw materials. The prepared substrate has stable high-cation environment, resistance of seedlings to hyperosmosis is continuously trained, and seedling raising substrate products suitable for heavy-salt soil regions are developed. The salinity of transplanted soil, which can be borne by plant seedlings cultivated by the products, is increased by 50% to 200%.

The invention provides a swine pasteurellosis vaccine enrichment medium, and relates to the field of biology. The swine pasteurellosis vaccine enrichment medium is prepared from the following raw materials by weight: 20.0g of meat liver and stomach membrane digestive juice powder, 8.0g of bovine pancreas digestive juice, 10.0g of peptone, 5.0g of NaCl, 1.0g of a growth promoting factor, and purified water added to 1000ml. A preparation method of the swine pasteurellosis vaccine enrichment medium comprises the following steps: adding all the raw materials for preparing the swine pasteurellosisvaccine enrichment medium into purified water, fully dissolving the raw materials, and carrying out sterilization at 121 DEG C for 15 minutes for later use. The invention relates to an application ofa synthetic dry powder culture medium in swine pasteurellosis vaccine production. The synthetic dry powder culture medium has the beneficial effects that commercial raw materials can be selected, theculture medium is clear, the number of cultured bacteria is high, and the freeze-drying survival rate of the bacteria is increased.

The invention relates to the field of water treatment, in particular to a water treatment system, which comprises an anaerobic reaction device and an aerobic reaction device, wherein the anaerobic reaction device is in fluid communication with the aerobic reaction device; the anaerobic reaction device comprises an anaerobic reaction tank body and a grid for dividing an internal space of the anaerobic reaction tank body into an isolated area and a filler area which are in fluid communication with each other; a water inlet of the anaerobic reaction device is positioned in the isolated area; a water outlet of the anaerobic reaction device is positioned in the filler area; an anaerobic reaction filler is arranged in the filler area. According to the water treatment system, systems for an A2O water treatment method can be effectively integrated, and in addition, air can float to wash the surface of a filter membrane, so that the effects of greatly reducing the energy consumption and saving an occupied area can be achieved.

The invention relates to the field of food processing and the field of biotechnology, and discloses a lactic acid bacteria stabilizer, a lactic acid bacteria beverage and a preparation method. The lactic acid bacteria stabilizer comprises low-ester pectin, medium-substitution starch phosphate, tragacanth, a metal ion regulator, a carbon source, an antioxidant and an osmotic pressure regulator. Thepreparation method of the lactic acid bacteria stabilizer comprises the following steps: firstly, adding low-ester pectin and tragacanth into water at the temperature of 75-85 DEG C, heating to 90-100 DEG C, and stirring for dissolving to obtain a mixed solution; and cooling the mixed solution to 30-40 DEG C, and adding other components to obtain the lactic acid bacteria stabilizer. The low-esterpectin, the medium-substitution starch phosphate and the tragacanth are mixed to form a copolymer, the lactic acid bacteria can be partially embedded and used in the lactic acid bacteria beverage, the stability of the lactic acid bacteria beverage can be improved, the storage stability of the lactic acid bacteria can be improved, and the survival time of the lactic acid bacteria can be prolonged.

The invention provides mesenchymal stem cells combined with traditional Chinese medicines for activating amplification, and a preparation method of the mesenchymal stem cells comprises the following steps: (1) pretreatment: digesting tissue pieces with pancreatin and IV collagenase, separating primary mesenchymal stem cells; (2) culture: adding the cells obtained in the step (1) to a low sugar DMEM culture medium containing components of 50-80mg/mL hemianthus micranthemoides extract, 50-80mg/mL Chinese gall extract, 1.5-3mmol/mL L-glutamine and 1.5-3ng/mL bFGF, culturing in a wet incubator of 37 DEG C with 5% CO2, changing the culture medium once every 3-4 days; and (3) collection of cells and subculture. Shown by in vitro tests, the traditional Chinese medicines activated mesenchymal stem cells can effectively inhibit reproduction of HBV and promote repair of damaged hepatic cells.

The invention discloses a preparation method of a thalassia hemperichii protoplast and belongs to the field of plant cell engineering. The preparation method comprises the following steps: S1, takingyoung leaves of thalassia hemperichii, washing them with sterile seawater, and temporarily culturing them to obtain a raw material 1; S2, putting the raw material 1 obtained in the step S1 into a pretreatment liquid for soaking to obtain a raw material 2; S3, taking out the raw material 2 obtained in the step S2, sucking dry water, cutting them into blocks, adding enzyme liquid for enzymolysis, and obtaining enzymolysis tissues; S4, filtering the enzymolysis tissue obtained in the step S3 to obtain filtrate; S5, centrifuging the filtrate obtained in the step S4, discarding the supernatant, suspending the precipitate with a resuspension solution, centrifuging, and discarding the supernatant to obtain a precipitate; S6, suspending the precipitate obtained in S5 with a resuspension solution to obtain the thalassia hemperichii protoplast. The preparation method of the thalassia hemperichii protoplast is established for the first time, and the thalassia hemperichii protoplast obtained by the method is high in yield and high in survival rate.

The invention discloses an efficient water-soothing hydrogel mask and a preparation method thereof, and relates to the technical field of cosmetics. The mask comprises the following components in percentage by weight: 1-10% of an active matter component, 0.1-7% of an oil phase component, 0.1-2% of an anti-corrosion component, 10-50% of a water phase component and the balance of water, wherein the water phase component comprises dihydroxyaluminum aminoacetate, allantoin, dipotassium glycyrrhizinate, EDTA-disodium, tartaric acid, glycerol, butanediol, and sodium polyacrylate. According to the efficient water-soothing hydrogel mask provided by the invention, under the action of body temperature and skin surface water molecules, internal physical crosslinking of solid essence is opened, essence molecules are released layer by layer and orderly permeate into the deep part of the skin, and the efficient water-soothing hydrogel mask can achieve water-soothing without irritation and is long in lasting time. Hydrogel essence is not easy to volatilize, does not suck back, always keeps stable osmotic pressure, and ensures that the essence orderly penetrates into the inner layer of the skin.

The invention discloses an immune cell cryopreservation liquid, which comprises the following components: 0.02 to 0.06 mg/mL of dimethyl sulfoxide, 1 to 6 mg/mL of cucurbitacine, 0.01 to 0.03 mL/mL of gleditsia sinensis polysaccharide, 1 to 2 mg/mL of lentinan, 1 to 5 [mu]g/mL of polyglycerol fatty acid ester, 0.01 to 0.03 mg/mL of polyethylene glycol, 10 to 16 mg/mL of non-essential amino acid and 12 to 20 mg/mL of human serum albumin. The invention discloses an immune cell cryopreservation method which comprises the following steps: uniformly mixing peripheral blood mononuclear cells with the immune cell cryopreservation liquid to obtain a cell suspension, transferring the cell suspension into a sterile cryopreservation tube, then putting the cell suspension into a cryopreservation box, carrying out programmed cooling to-70 DEG C, carrying out cryopreservation for 10-15 hours, and then transferring the cell suspension into liquid nitrogen for cryopreservation, wherein the concentration of the cells in the cell suspension is (2-4) * 10<7> cells/mL.