Chinese sturgen artificial insemination method and used diluent

Abstract

The artificial insemination method for Chinese sturgeon adopts diluent A and diluent B as insemination medium and adopts double wet method to make operation. For the first time it uses the diluent A to wash ova and remove ovarian liquid, and for the second time it uses the diluent B to activate ova and spermatozoa and make the spermatozoa be uniformly dispersed to implement insemination. The described diluent includes diluent A and diluent B. The diluent A is made up by using NaCl, Kcl, CaCl2 2H2O, MgCl 6H2O, NaHCo3, Na2Co3, NaOH, citricacid, penicillin, streptomycin and distilled water, and the diluent B is obtained by using distilled water whose volume is 25 times that of diluent A to dilute the diluent A.

Description

Technical field: [0001] The invention relates to fish artificial insemination technology. Background technique: [0002] The Chinese sturgeon is a large fish that migrates between rivers and seas. Due to factors such as the construction of the Yangtze River water conservancy project, the aggravation of water pollution, and the increase in fishing intensity, the population of Chinese sturgeon has declined sharply, and it has now become a national first-class protected animal. It has become an important topic of aquatic science research to improve the artificial reproduction and artificial proliferation and release technology of Chinese sturgeon to help the recovery of the population. [0003] The artificial breeding of Chinese sturgeon requires Chinese sturgeon broodstock. Due to the reduction of government-licensed fishing, very few Chinese sturgeon broodstock can be obtained, and the male broodstock among them is even rarer. Therefore, improving the fertilization rate is ...

AI Technical Summary

Problems solved by technology

[0006] 1. Directly use Yangtze River water or other fresh water as the insemination medium for artificial insemination of Chinese sturgeon. Due to the intensification of industrial activities, various production waste liquids and residues are discharged into the water and underground, which makes the composition of different water sources different and complex, and the impact of different water quality The factors of the activation rate of sperm and eggs are quite different, especially the pH value is not suitable, which leads to the inhibition of the activity of certain enzymes in the sperm, resulting in the loss of the sperm's ability to fertilize

[0007] 2. Once the fresh water used as the insemination medium is polluted (such as heavy metals, organic phosphorus, etc.), it will have a toxic effect on eggs and sperm, making them lose their vitality, directly affecting the fertilization rate, and increasing the distortion of the embryo

[0008] 3. Artificial insemination, squeeze eggs to bring out a lot of ovarian fluid, and the ovarian fluid of Chinese sturgeon is very viscous, wrapped together with the eggs, using dry insemination, when the semen is added, many sperm are stuck by the ovarian fluid, and fresh water is added to activate After that, it cannot move quickly to penetrate the fertilization hole of the egg, so that some eggs cannot be fertilized

[0009] 4. During the operation, the fresh water composition, osmotic pressure, and pH value used as the insemination medium are uncertain, and the dosage and time control of each link are not accurate, and there is randomness

However, there are few reports on artificial insemination dilutions for fish, especially river-sea migratory fish.